Molecular markers in close linkage with specific resistance gene for capsicum phytophthora blight of root rot and application thereof

A technology of molecular markers and resistance genes, applied in the field of molecular biology, can solve problems such as the location of specific resistance genes for root rot blight that have not been seen, and achieve the effect of high-throughput applications

Active Publication Date: 2015-06-24
INST OF VEGETABLES GUANGDONG PROV ACAD OF AGRI SCI +1
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  • Abstract
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  • Claims
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Problems solved by technology

Quantitative resistance is controlled by multiple genes. The main root rot resistance gene identified and mapped by a number of research results is located on chromosome 5 of pepper (Quirin et al., 2005; Truong et al., 2013); The resi

Method used

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  • Molecular markers in close linkage with specific resistance gene for capsicum phytophthora blight of root rot and application thereof
  • Molecular markers in close linkage with specific resistance gene for capsicum phytophthora blight of root rot and application thereof
  • Molecular markers in close linkage with specific resistance gene for capsicum phytophthora blight of root rot and application thereof

Examples

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Embodiment Construction

[0026] The present invention will be further described below in conjunction with the examples, but not limited thereto.

[0027] The molecular biology experimental techniques used in the following examples include DNA extraction, PCR amplification, PAGE gel electrophoresis, enzyme digestion, transformation and other experiments. Unless otherwise specified, they are usually operated according to conventional methods. For details, please refer to Molecular Cloning Experiments Guide" (Third Edition) (Sambrook J, Russell DW, Janssen K, Argentine J. Huang Peitang et al. translation, 2002, Beijing: Science Press), or according to the conditions suggested by the manufacturer.

[0028] 1. Genetic population construction and genetic analysis

[0029] 1. Test materials

[0030] Plant materials: The internationally recognized pepper disease-resistant material CM334 and the high-generation homozygous susceptible material 10399 were donated by Professor Paul W. Bosland, an expert in pep...

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Abstract

The invention discloses two molecular markers in close linkage with a specific resistance gene for capsicum phytophthora blight of root rot and application thereof. Each molecular marker contains a nucleotide sequence shown in SEQ ID NO. 1 and named as P52-11-21, or contains a nucleotide sequence shown in SEQ ID NO. 2 and named as P52-11-41. The molecular markers are in close linkage with the specific resistance gene for phytophthora blight of root rot, have genetic distances of 1.5cM and 1.1cM respectively, and can be directly used for establishing an assistant breeding system for the molecular markers for capsicum phytophthora blight of root rot. The molecular markers disclosed by the invention and molecular marker amplification primers can be applied to breeding practice for capsicum phytophthora blight of root rot simply and rapidly with a high flux, and lay a good foundation for the cloning and functional researches of the specific resistance gene for capsicum phytophthora blight of root rot.

Description

technical field [0001] The invention belongs to the field of molecular biology and relates to molecular markers, in particular to two molecular markers closely linked with pepper root rot blight specific resistance genes and applications thereof. Background technique [0002] Pepper blight is caused by Phytophthora capsici ( Phytophthora capsici Leon.) is a devastating soil-borne disease (Leonian, 1922) that is widespread worldwide. Due to the influence of high temperature and high humidity climate conditions in Guangdong area, pepper blight is particularly serious, and pepper production often suffers heavy losses. [0003] Pepper blight is manifested as different diseases such as root rot, fruit rot, stem blight and leaf blight according to the different infection parts of the pathogen (Risttaino, 1990). The genetic mechanisms of resistance vary (Barksdale et al., 1984; Sy et al., 2005). The occurrence of pepper root rot disease usually directly leads to irreversible de...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 徐晓美巢娟李颖罗少波吴廷全黄智文
Owner INST OF VEGETABLES GUANGDONG PROV ACAD OF AGRI SCI
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