Molecular markers in close linkage with specific resistance gene for capsicum phytophthora blight of root rot and application thereof

Abstract

The invention discloses two molecular markers in close linkage with a specific resistance gene for capsicum phytophthora blight of root rot and application thereof. Each molecular marker contains a nucleotide sequence shown in SEQ ID NO. 1 and named as P52-11-21, or contains a nucleotide sequence shown in SEQ ID NO. 2 and named as P52-11-41. The molecular markers are in close linkage with the specific resistance gene for phytophthora blight of root rot, have genetic distances of 1.5cM and 1.1cM respectively, and can be directly used for establishing an assistant breeding system for the molecular markers for capsicum phytophthora blight of root rot. The molecular markers disclosed by the invention and molecular marker amplification primers can be applied to breeding practice for capsicum phytophthora blight of root rot simply and rapidly with a high flux, and lay a good foundation for the cloning and functional researches of the specific resistance gene for capsicum phytophthora blight of root rot.

Description

technical field [0001] The invention belongs to the field of molecular biology and relates to molecular markers, in particular to two molecular markers closely linked with pepper root rot blight specific resistance genes and applications thereof. Background technique [0002] Pepper blight is caused by Phytophthora capsici ( Phytophthora capsici Leon.) is a devastating soil-borne disease (Leonian, 1922) that is widespread worldwide. Due to the influence of high temperature and high humidity climate conditions in Guangdong area, pepper blight is particularly serious, and pepper production often suffers heavy losses. [0003] Pepper blight is manifested as different diseases such as root rot, fruit rot, stem blight and leaf blight according to the different infection parts of the pathogen (Risttaino, 1990). The genetic mechanisms of resistance vary (Barksdale et al., 1984; Sy et al., 2005). The occurrence of pepper root rot disease usually directly leads to irreversible de...

AI Technical Summary

Problems solved by technology

Quantitative resistance is controlled by multiple genes. The main root rot resistance gene identified and mapped by a number of research results is located on chromosome 5 of pepper (Quirin et al., 2005; Truong et al., 2013); The resistance is controlled by a single gene, showing complete resistance to a specific strain (Monroy-Barbosa and Bosland, 2008). So far, there has been no report on the location of a specific resistance gene for root rot at home and abroad.

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