Diluent of carboxylated magnetic particles and preparation method of diluent

Abstract

The invention discloses diluent of carboxylated magnetic particles and a preparation method of the diluent. The diluent is prepared from, by weight, 39-55 parts of N-(2- ethoxyl)piperazine-N'-2-ethanesulfonic acid, 28-38 parts of dipotassium phosphate, 9-18 parts of ethylene diamine tetraacetic acid, 11-29 parts of zinc chloride, 7-12 parts of magnesium chloride, 6-13 parts of heparin sodium, 15-22 parts of saccharose, 0.1-0.3 part of hydrochloric acid, 4-12 parts of glucose, 1-3 parts of bovine serum albumin, 0.2-0.6 part of mouse serums, 0.3-0.7 part of glycerin, 0.2-0.4 part of Proclin 300,0.4-0.8 part of Tween-20 and 100-300 parts of deionized water. According to the diluent of the carboxylated magnetic particles and the preparation method of the diluent, through synergistic effect ofall the components, the diluent of the carboxylated magnetic particles is prepared and used for diluting carboxylated magnetic particle mother liquor into a working solution, the working solution canbe stored for a long time, and the specificity, stability, repeatability and other performance of a reagent are not influenced.

Description

technical field [0001] The invention relates to the field of chemical immunoluminescent reagents, in particular to a diluent of carboxylated magnetic particles and a preparation method thereof. Background technique [0002] With the development of science and technology, chemiluminescence technology has been advanced at the same time. So far, chemiluminescence analysis has developed rapidly in the field of biomedicine, mainly because it does not require an external light source during the analysis process, which can avoid the interference of other light sources during the detection process and improve its sensitivity. The immobilization method is particularly important in chemiluminescent immunoassays, mainly to wrap enzymes, antigens, antibodies, etc. on some polymer materials. A suitable solid phase carrier is the most effective way to improve the stability, sensitivity, and specificity of the immunoassay method. one of the ways. Among them, carboxylated magnetic particl...

AI Technical Summary

Problems solved by technology

Among them, carboxylated magnetic particles are suitable for the binding reaction of antibodies, and at the same time have a large specific surface area and a large number of bindings; however, many companies mainly focus on the research and development of magnetic particles, and ignore the buffer system after the magnetic particles are formulated into working solutions. An important role in the antibody-antigen reaction process, which affects the performance indicators of the reagent