Method for preparing nano anticancer probe of zirconium-porphyrin metal organic framework material

Abstract

Provided is a method for preparing a nano anticancer probe of a zirconium-porphyrin metal organic framework material. Anhydrous zirconium chloride, meso-4-carboxyphenyl porphyrin, cetyl trimethyl ammonium bromide, benzoic acid and polyethylene glycol are added into N,N-dimethylformamide for ultrasonic dissolving, and an obtained mixed solution is subjected to a heating reaction till purple sediment is precipitated out at the bottom; after centrifugal separation, the sediment is washed with N,N-dimethylformamide, ethyl alcohol and water, and freeze drying is carried out to obtain a nano anticancer probe 1; chemotherapeutic medicine is dissolved in a 4-hydroxyethylpiperazine ethane sulfonic acid buffer solution, the nano anticancer probe 1 is added, centrifugal separation is carried out after stirring is carried out at normal temperature, washing is carried with a 4-hydroxyethylpiperazine ethane sulfonic acid buffer solution, freeze drying is carried out, and a nano anticancer probe 2 is obtained. The method has the advantages that the process is simple, and implementation is easy; the nano anticancer probe is used as an imaging induced synergic treatment system in the field of biomedicine, and meanwhile cancer is detected and treated.

Description

technical field [0001] The invention belongs to the field of preparation of nanometer materials and the field of integrated application of diagnosis and treatment, and in particular relates to the preparation of a nanometer zirconium-porphyrin metal organic framework material and the synergistic treatment application of chemotherapy and photodynamic therapy guided by fluorescence imaging of the material. Background technique [0002] Cancer has become a disease with high morbidity and mortality, and safe and effective cancer treatment methods have attracted widespread attention. Among them, the imaging-guided treatment system, as a comprehensive therapy that integrates imaging detection, monitoring and treatment methods, has guided a new direction for cancer treatment. Fluorescence imaging, especially red light and near-infrared light imaging in the "biological window", has become a non-tissue damage imaging technology due to its high penetration depth, high signal-to-noise ...

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Problems solved by technology

However, such luminescent materials have high toxicity (heavy metal leakage) and are difficult to overcome; noble metal nanodots and noble metal clusters have strong chemical stability and are easy to modify, such as, 3) S.Jiang, K.Y.Win, S.Liu, C.P.Teng, Y. Zheng, M. Y. Han, Nanoscale 2013, 5, 3127; 4) F. He, G. Yang, P. Yang, Y. Yu, R. Lv, C. Li, Y. Dai, S. Gai, J. Lin, Adv. Funct. Mater. 2015, 25, 3966

However, such materials have poor metabolism in vivo and low drug load, which is not conducive to effective treatment; the size of organic polymer micelles is adjustable and the load is large, such as, 5) S.Chang, T.Si, S.Zhang ,M.A.Merrick,D.E Cohn,R.X.Xu,Ultrason.Sonochem.2016,28,31; 6)A.Topete,S.Barbosa,P.Taboada,J.Appl.Polym.Sci.2015,132

However, its thermodynamic stability is poor, it is easy to decompose, and it is not convenient for in vivo transmission, which reduces the therapeutic effect.

The low biocompatibility of the above materials, complex synthesis and cumbersome steps also limit their application in the field of bioimaging

At the same time, the above materials are a single treatment system, lacking an imaging-guided synergistic treatment system

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