Stable and sensitive fibronectin detection reagent

A fibronectin and detection reagent technology, which is applied in biological testing, measuring devices, material testing products, etc., can solve the problems of difficult popularization, poor stability, time-consuming and labor-intensive, etc., and achieves good accuracy and stability to meet clinical needs. , easy to use effect

Active Publication Date: 2017-02-01
山东博科诊断科技有限公司
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, rocket electrophoresis and enzyme-linked immunoassay (ELISA) require specific equipment, which is time-consuming and laborious, and are not easy to be popularized and used; while immunoturbidimetric method is simple and convenient to use and easy to automate, but has disadvantages such as low sensitivity and poor stability.

Method used

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  • Stable and sensitive fibronectin detection reagent
  • Stable and sensitive fibronectin detection reagent
  • Stable and sensitive fibronectin detection reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The detection reagents for fibronectin described in this example include reagent R1 and reagent R2:

[0043] The components of reagent R1 are:

[0044] HEPES (4-hydroxyethylpiperazineethanesulfonic acid) buffer (pH=7.6, 25°C) ············ 50mmol / L,

[0045] γ-Fe 2 o 3 / Polythiophene (γ-Fe 2 o 3 -PTP) composite nanoparticles ················ 1.0% (W / V),

[0046] Fluorocarbon Surfactant FC-4430 ···································· 2g / L,

[0047] Methylisothiazolinone (MIT) ······································ 0.1g / L;

[0048] The components of reagent R2 are:

[0049] HEPES (4-hydroxyethylpiperazineethanesulfonic acid) buffer (pH=7.6, 25°C) ············ 80mmol / L,

[0050] Goat anti-human fibronectin antibody ··································· 15% (W / V),

[0051] gelatin ····················································· 0.2% (W / V),

[0052] xanthan gum ··················································· 0.2% (W / V),

[0053] Sodium chloride ········...

Embodiment 2

[0061] The fibronectin detection reagent described in this example includes reagent R1 and reagent R2 (the detection method is the same as that in Example 1):

[0062] The components of reagent R1 are:

[0063] HEPES (4-hydroxyethylpiperazineethanesulfonic acid) buffer (pH=7.6, 25°C) ············ 50mmol / L,

[0064] γ-Fe 2 o 3 / Polythiophene (γ-Fe 2 o 3 -PTP) composite nanoparticles ················ 1.5% (W / V),

[0065] Fluorocarbon Surfactant FC-4430 ···································· 2g / L,

[0066] Methylisothiazolinone (MIT) ······································ 0.1g / L;

[0067] The components of reagent R2 are:

[0068] HEPES (4-hydroxyethylpiperazineethanesulfonic acid) buffer (pH=7.6, 25°C) ············ 80mmol / L,

[0069] Goat anti-human fibronectin antibody ··································· 20% (W / V),

[0070] gelatin ····················································· 0.5% (W / V),

[0071] xanthan gum ·············································...

Embodiment 3

[0078] The fibronectin detection reagent described in this example includes reagent R1 and reagent R2 (the detection method is the same as that in Example 1):

[0079] The components of reagent R1 are:

[0080] HEPES (4-hydroxyethylpiperazineethanesulfonic acid) buffer (pH=7.6, 25°C) ············ 50mmol / L,

[0081] γ-Fe 2 o 3 / Polythiophene (γ-Fe 2 o 3 -PTP) composite nanoparticles ················ 2.0% (W / V),

[0082] Fluorocarbon Surfactant FC-4430 ···································· 2g / L,

[0083] Methylisothiazolinone (MIT) ······································ 0.1g / L;

[0084] The components of reagent R2 are:

[0085] HEPES (4-hydroxyethylpiperazineethanesulfonic acid) buffer (pH=7.6, 25°C) ············ 80mmol / L,

[0086] Goat anti-human fibronectin antibody ··································· 35% (W / V),

[0087] gelatin ····················································· 0.8% (W / V),

[0088] xanthan gum ·············································...

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Abstract

The invention relates to the field of a serum fibronectin detection technology, in particular to a fibronectin detection reagent. A reagent R 1 contains buffer solution, gamma-Fe2O3/polythiophene (gamma-Fe2O3/PTP) composite nanoparticles, fluorocarbon surfactant FC-4430 and preservative; a reagent R 2 contains buffer solution, goat anti human IgG fibronectin antibody, gelatin, xanthan glue, sodium chloride, mycose, PEG-6000, fluorocarbon surfactant FC-4430 and preservative. According to the fibronectin detection reagent, the reacting system is optimized, the stability of the reagent can be improved significantly through the adoption of HEPES (4-hydroxyethylpiperazine ethane sulfonic acid) buffer solution and the addition of a variety of stabilizers, such as sodium chloride, mycose, PEG-6000, gelatin and xanthan glue; the addition of gamma-Fe2O3/polythiophene (gamma-Fe2O3/PTP) composite nanoparticles in the reagent can adsorb the antigen-antibody complex through electrostatic adsorption during the detection, thus the sensitivity and accuracy degree of the reagent can be enhanced significantly. Moreover, the optimized novel fluorocarbon surfactant FC-4430 can promote and maintain the stability of the antibody, prevent the system from being muddied, and further enhance the stability of the reagent.

Description

technical field [0001] The invention relates to the technical field of fibronectin (Fn) detection, in particular to a fibronectin (Fn) detection reagent and a detection method using the detection reagent. Background technique [0002] Fibronectin (also known as fibronectin, English abbreviation Fn), widely exists in animal tissues and tissue fluids, is composed of two almost identical subunits linked by disulfide bonds, has non-immune opsonization and A high molecular glycoprotein with various biological activities. Elevated serum or plasma fibronectin is common in cerebral infarction and pregnancy-induced hypertension, and decreased in liver cirrhosis, malnutrition, stroke, burns, toxic respiratory failure, and pulmonary infection. In addition, the determination of fibronectin (Fn) is helpful for the differential diagnosis of cerebral thrombosis and cerebral hemorrhage, and the dynamic observation of plasma Fn content in patients with cerebrovascular diseases is helpful fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/536
CPCG01N33/536G01N33/6893G01N2333/78
Inventor 李志明赵民高敏王美丽
Owner 山东博科诊断科技有限公司
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