The invention relates to a method for cultivating rhodiola rosea bag material healthcare pleurotus nebrodensis. A cultivation medium is prepared by, by weight, 20-30 parts of lotus seed hulls, 20-30 parts of cotton stalks, 10-20 parts of sesame stalks, 8-10 parts of bran, 5-6 parts of casava powder, 0.8-1.1 parts of white sugar, 4-5 parts of vinegar residues, 0.5-0.8 part of silkworm excrement, 1-2 parts of salvia miltiorrhizae powder, 2-3 parts of Japanese metaplexis pericarp powder, 1-3 parts of pepper leaf powder, 1-2 parts of rhodiola rosea powder, 2-3 parts of red pine leaf powder, 2-3 parts of Kangxian flower powder, 1-2 parts of bentonite, 1-2 parts of gypsum powder, 2-3 parts of quicklime, 0.2-0.3 part of urea and 10-12 parts of bone soup. The method includes mixing the components according to a proportion, adding water, well mixing, bagging, sterilizing, grafting, producing bacteria in a bacterium producing chamber, replenishing nutrient solution and fruiting pleurotus nebrodensis. The pleurotus nebrodensis cultivated by the method is nutrient-rich and high in content of trace elements, vitamins and calcium and has a rhodiola rosea bag material healthcare function, anti-infection capability is high during pleurotus nebrodensis fruiting, and pleurotus nebrodensis yield is high.