A monkey embryo kidney epithelial cell marc-145 suspension adapted strain and its application in culturing PRRS virus and producing PRRS virus vaccine

A technology of PRRS virus and cell suspension, applied in animal cells, virus/phage, vertebrate cells, etc., can solve the problems of limited increase in cell density, easy pollution, high labor intensity, etc., and solve the problem of batch-to-batch variation , Reduce production costs, simplify the production process
CN104894054BActive Publication Date: 2017-12-22郑州爱科生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
郑州爱科生物科技有限公司
Publication Date
2017-12-22

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Abstract

The invention relates to a monkey embryo kidney epithelial cell Marc‑145 suspension adaptation strain and its application in cultivating PRRS virus and producing PRRS virus vaccine, belonging to the field of biotechnology. The present invention belongs to a disclosed monkey embryonic kidney epithelial cell Marc‑145 suspension adaptation strain, which is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: C201542. The invention also discloses the use of the Marc‑145 cell suspension adaptation Strain culture method of PRRS virus. The Marc-145 cell suspension adaptation strain provided by the present invention has realized the full suspension culture of Marc-145 cells in the culture medium, thereby adopting the Marc-145 cell suspension adaptation strain of the present invention to produce PRRS virus, easy to use in industrial production The bioreactor is amplified step by step to realize mass production of PRRS vaccines. Compared with the existing spinning bottle and carrier suspension culture technology, the present invention simplifies the production process, shortens the production cycle, reduces production costs, and further improves PRRS vaccine production. quality and production of vaccines against the disease.
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Description

technical field

[0001] The invention relates to a monkey embryo kidney epithelial cell Marc-145 suspension adaptation strain and its application in cultivating PRRS virus and producing PRRS virus vaccine, belonging to the field of biotechnology, more specifically, the invention relates to a method using A method for the production of PRRS virus by fully suspending Marc-145 cells in a bioreactor. Background technique

[0002] Marc-145 cells are monkey embryonic kidney epithelial cells, which are derived from monkey kidney cells and are clones of mother cells (MA104 cells). They grow anchorage-dependently and can be continuously subcultured without tumorigenicity. Suspension-adapted strains can be obtained through suspension adaptation, which can grow in suspension without relying on the carrier. The cells are particularly sensitive to porcine reproductive and respiratory syndrome virus (PRRSV) and are currently widely used in the production of PRRS vaccines.

[0003] Due to...

Claims

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