Large-area culture roller bottle for biological cell culture

Abstract

The invention discloses a large-area culture roller bottle for biological cell culture. A plurality of annular culture cavities are formed through the cooperation of a plurality of inner ring bottles, so that the actual culture area is increased in a disguised mode; the multiple inner ring bottles are separated, so that the culture of different cells can be conducted at the same time; and nutrient solutions in the annular culture cavities are prevented from overflowing by utilizing an inner bent part and an outer bent part. The large-area culture roller bottle is characterized in that one end of a top sealing cover covers one end of an outer culture bottle; an air hole is formed in the middle of one end of the top sealing cover; an air-permeable film is arranged on the air hole; the air-permeable film is made of transparent materials; the other end of the outer culture bottle extends in the axial direction of the outer culture bottle, then is bent towards the outer portion of the outer culture bottle and extends in the axial direction of the outer culture bottle; the outer culture bottle is bent to form the outer bent part; and the bending angle of the outer bent part ranges from 45 degrees to 75 degrees.

Description

technical field [0001] The invention discloses a large-area culture roller bottle for biological cell culture, relates to a culture roller bottle capable of large-area culture for biological cells, and belongs to the field of bioengineering. In particular, it relates to a method of forming multiple ring-shaped culture chambers through the cooperation of multiple inner-ring bottles, which increases the actual culture area in a disguised form, and the multiple inner-ring bottles are separated, so that different cells can be cultured at the same time. The outer bending part prevents the large-area culture roller bottle from overflowing the nutrient solution inside the annular culture chamber. Background technique [0002] Roller bottle, a container for cell culture, is mainly used in laboratory cell research and industrial production of biological products. The roller bottle is mainly added with a specific ratio of medium and cell suspension, and then the bottle cap is closed, ...

AI Technical Summary

Problems solved by technology

[0002] Roller bottle, a container for cell culture, is mainly used in laboratory cell research and industrial production of biological products. The roller bottle is mainly added with a specific ratio of medium and cell suspension, and then the bottle cap is closed, and the roller bottle is placed on the roller bottle Rolling on the machine makes the cells in the liquid stick to the wall, so that the cells cultured in the roller bottle do not have to be immersed in the culture solution all the time, so as to facilitate cell respiration and material exchange. The attached area is only a single inner wall in the roller bottle, the cultivation area is limited, the remaining space inside the bottle is not utilized, and the space utilization rate is low. In production, it is often necessary to equip a large number of roller bottles to meet the production output. The increase in the number of roller bottles needs to be equipped with a roller bottle machine, which greatly increases the production cost and the production space used by the equipment.

[0003] The notification number CN209493585U discloses a roller bottle for cell culture, including a bottle body, a bottle tube, a bottle cap, a driving assembly and a stirring assembly, and the stirring assembly includes a rotating shaft, a first magnet, a slide tube and two stirring units, and the stirring unit It includes a support rod and a number of stirring rods. The driving assembly includes a second magnet and two sliding units. The sliding unit includes a sliding bar, a slider, an insertion tube and an insertion rod. During the actual cultivation process of the roller bottle, only the roller bottle is close to the inner wall The space at the place is used, the cultivation area is small, and the actual utilization rate of the space in the bottle is low

[0004] Notification number CN208378903U discloses a cell culture roller bottle for increasing the cell culture area, including a bottle body and a bottleneck. The bottle body includes a bottle bottom, a bottle inner wall, a bottle cavity and a bottle top, and the bottle cavity is provided with at least one deflector , the perimeter of at least one deflector is connected to the bottom, inner wall or top of the bottle; the thickness of the deflector is 1 mm to 4 mm, the length is more than 1 / 2 of the depth of the inner cavity of the bottle, and the width is smaller than the radius of the bottle body , the surface of the plate spreads along the radial direction of the bottle body. Although the roller bottle increases the cultivation area in the bottle by adding deflectors, the actual utilization rate of the space in the bottle is still low, and the existence of the deflectors also leads to rolling. When the bottle is rotated, the internal culture solution is prone to violent flow, which affects the uniformity of the culture solution in the bottle

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