Application of static constant magnetic field for prolonging hold time of primary cell

Abstract

The invention discloses application of static constant magnetic field used for prolonging hold time of primary cell, including that cells are placed in a magnetic field to be cultured and the magnetic field is arranged on the outer wall close to the cells. Concretely, the magnetic field is arranged at the bottom of a cell culture plate when the cell is cultured by adopting the cell culture plate; the magnetic filed is arranged at the bottom of a cell culture bottle when the cell is cultured by adopting the cell culture bottle; and the magnetic field is arranged on the outer wall of a roller bottle when the cell is cultured by adopting the roller bottle. The magnetic field is a static constant one, and magnetic force is 500-3000 Gauss. The primary cell can be histocytes coming from all animals including human, such as hepatocyte, nephrocyte, lens cell and testicular cell. The invention adopts a manner of adding a magnetic field into the cell culture environment to prolong hold time of primary cell in vitro, the hold time of cell can be more than 30 days, and cell growth is vigorous, thus effect is good.

Description

technical field [0001] The invention relates to the application of a static constant magnetic field in prolonging the maintenance time of primary cells, belonging to the field of cell culture. Background technique [0002] In vitro cell culture is a commonly used research tool in many fields such as biology and medicine, among which the culture of diploid cells, especially primary cells, occupies an important position in related fields. At present, most primary cells are difficult to subculture, or even cannot be subcultured (human embryonic lung can be subcultured for 50-60 generations in vitro), and the maintenance time of primary cells cultured in vitro is usually 10-20 days. Therefore, there is an urgent need for a method that can prolong the maintenance time of cells. [0003] In the prior art, in order to prolong the survival time of the primary cells, relevant technical personnel often take methods of lowering the culture temperature and reducing the nutrient content...

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Problems solved by technology

[0003] In the prior art, in order to prolong the survival time of the primary cells, relevant technical personnel often take methods of lowering the culture temperature and reducing the nutrient content in the culture solution (the culture solution that reduces the nutrient content is called a maintenance solution, such as MEM containing 2% newborn bovine serum. Culture medium) and other methods, there is no report on prolonging the maintenance time of primary cells with a static constant magnetic field

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