Application of baby hamster kidney(BHK)-21 cell serum-free suspension culture technology in foot-and-mouth disease vaccine production

Abstract

The invention discloses application of a baby hamster kidney(BHK)-21 cell serum-free suspension culture technology in foot-and-mouth disease vaccine production, which comprises the following steps of: 1) performing cell recovery; 2) performing reactor culture and cell amplification culture; and 3) inoculating foot-and-mouth disease virus seed venom and collecting the venom. A process for producing foot-and-mouth disease inactivated vaccines by culturing the BHK-21 cells through serum-free suspension culture and a step-by-step cell amplification method make the production period f the foot-and-mouth disease vaccines shortened and yield increased, and ensure stable quality and obvious benefit. The production process reduces the using amount of a culture medium, and the amount of the collected virus liquid is the culture medium consumption amount, while the culture medium consumption amount in a roller bottle production process is 2 times higher than the amount of the collected virus liquid, and bovine serum which is about 5 percent of the culture medium amount is needed.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals, and specifically relates to the application of BHK-21 cell serum-free suspension culture technology in the production of foot-and-mouth disease vaccine. Background technique [0002] BHK-21 cells are Syrian hamster kidney cells, English name: Baby Hamster Kidney cell referred to as BHK-21 or BHK 21 , the cell line was established by Macpherson IA and Stoker MGP in 1961. It was derived from the kidneys of five 1-day-old hamsters of unidentified gender. Single-cell cloning was carried out in 1963. The original cell line is fibroblast, which grows in an anchorage-dependent manner, but can grow in suspension after numerous passages and domestication. This cell is widely used for virus proliferation and production of vaccines, such as foot-and-mouth disease vaccines, rabies vaccines, and Japanese encephalitis vaccines. Because the original BHK-21 cells are anchorage-dependent growth, 10 liters and 1...

AI Technical Summary

Problems solved by technology

The existing technological process is complicated and complicated, the cycle is long, the labor intensity is high, labor-intensive, the consumption of raw materials is large, and the quality is unstable, the difference between batches is large, and the prepared vaccine has large side effects after injection into animals.