Prepn process of brain protein hydrolysate injection

A technology for the preparation of brain protein hydrolyzate and injection, which is applied in the field of medicine, can solve the problems of increased cost, unrealistic chromatography process, and inability to produce qualified products, and achieve the effect of preventing oxidation

Inactive Publication Date: 2007-08-22
石海
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

But in practice, we cannot produce qualified products according to the reported process, mainly because the other substances in the standard [inspection] items are more than 15%, and the color, especially in the storage process, cannot meet the requirements of light yellow clear liquid
In addition, the degreasing step requires an explosion-proof workshop. On the one hand, it increases the cost, and on the other hand, it is really unnecessary in the process.
The chromatography process is not realistic in practice

Method used

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Examples

Experimental program
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Effect test

Embodiment Construction

[0017] Specific implementation examples

[0018] 1. Take 80kg of fresh pig brain, remove the fascia, wash it, add 1.5 times of water for injection, homogenize it with a colloid mill, put it in a sandwich pot, heat it to 85°C for 15 minutes, cool it to 42°C, and wash it with hydrochloric acid Adjust the pH value to about 3.5, add 2.4 kg of pepsin, stir well, pack in stainless steel barrels, hydrolyze in a water bath at 42±2°C for about 6 hours, take it out and place it in a -20°C freezer overnight to freeze thoroughly, and take it out the next day Naturally permeate at room temperature and absorb the supernatant. Take 1.6kg of trypsin and add 3.2kg of water to dissolve it, add 7g of calcium chloride, stir well and let it stand at room temperature for 1 hour, then hydrolyze at 42±2°C for 4 hours. Put the above-mentioned hydrolyzate in a sandwich pot and heat it to boiling for 20 minutes, then cool it rapidly to obtain the supernatant. The above supernatant was ultrafiltered on...

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Abstract

The brain protein hydrolysate injection is prepared with health swine brain and contains 16 kinds of free amino acids and small amount of peptides. It is used in treating sequelae of craniocerebral trauma and cerebrovascular disease, improving cerebral function, etc. It is prepared through washing, homogenizing, thermal changing, enzyme hydrolysis, freezing, ultrafiltering, sterilizing and other steps.

Description

Technical field: [0001] The invention belongs to the technical field of medicine Background technique [0002] Brain protein hydrolyzate injection is a sterilized aqueous solution prepared by enzymatic hydrolysis of healthy pig brains. Each 1ml contains 28.08-42.14mg of free amino acids and 5.49-6.71mg of total nitrogen (N). It is used for craniocerebral trauma , Sequelae of cerebrovascular disease accompanied by improvement of symptoms of memory loss and attention deficit disorder. It can help to improve brain dysfunction, and it is also used for protein deficiency and neurasthenia patients for cases of general protein digestion and absorption disorders. [0003] This variety was first imported from Austria, and later produced in China. There are many literature reports on its technological process, and its process is roughly the process of degreasing, hydrolysis, ultrafiltration, chromatography, and deployment of amino acids. But in practice, we cannot produce qualified...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/30A61K38/01A61K9/08C12P21/02A61P25/00
Inventor 石海
Owner 石海
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