Brain protein hydrolysate and production process of its freeze dried preparation

Abstract

The present invention relates to a kind of brain protein hydrolysate and the production process of its freeze dried preparation. The brain protein hydrolysate for injection is prepared with pig brain and through the steps of homogenating in a colloid mill to collect slurry, hydrolyzing with pepsase and pancreatin to collect supernatant, filtering with filer paper to collect filtrate, separating and purifying the filtrate with hydroxyapetite column, regulating peptide map and collecting object, ultrafiltering with membrane of intercepting molecular weight 8 KD to collecting filtrate, nitrogen and amino acid analysis and adding amino acid in the required amount, and fine filtering with 0.22 micron filtering membrane. The brain protein hydrolysate for injection may be further freeze dried to obtain freeze dried brain protein hydrolysate preparation. The production process is environment friendly, high in yield and low in cost.

Description

technical field [0001] The invention relates to a production method of cerebroprotein hydrolyzate, and also relates to a production method of cerebroprotein hydrolyzate freeze-dried preparation, belonging to the field of pharmacy. Background technique [0002] Brain tissue is rich in protein. For a long time, people have been researching to hydrolyze the protein of animal brain tissue into amino acids and low-molecular-weight polypeptides, which can be used to treat dysfunction caused by insufficient compensation of cerebral blood vessels, stroke, brain memory impairment and traumatic brain injury. Dysfunction, sequelae of cerebral oscillations and other diseases. [0003] Cerebroprotein hydrolyzate is also called cerebrolysin in China. As early as the early 1990s, domestic scholars conducted research on its technology, quality, pharmacological efficacy and clinical application, and published a large number of articles. [0004] Chinese invention patent document CN01130523....

AI Technical Summary

Problems solved by technology

The disadvantages are: (1) The feed liquid passes through the microporous membrane with a pore size of 0.5-1um and the ultrafiltration membrane with a cut-off molecular weight of 70,000 and 10,000 for many times, and the obtained ultrafiltrate makes the operation more cumbersome and the production cycle is long. Susceptible to microbial contamination during production

(2) The recovery rate of ultrafiltration concentration using ultrafiltration membrane is low: the process adopts the ultrafiltration membrane with a cut-off molecular weight of 1000 to concentrate, because the effective active ingredient of cerebroprotein hydrolyzate is a small molecule polypeptide, it will be concentrated in the ultrafiltration concentration process. There are different degrees of permeation loss, and the yield is greatly reduced

Its disadvantages are: (1) long production cycle, large investment in equipment, and low production efficiency; trypsin hydrolysis for 30 hours at pH8. Hydrolysis with trypsin, the hydrolysis is not complete, and the solid-liquid separation is difficult, and the loss in the production process is large; the production efficiency is low when the vacuum concentration step is used, which is not conducive to the control of the quality of the drug

(2), use a large amount of organic chemical solvents, acetone and ether have extremely low boiling points, are flammable, explosive, poisonous dangerous chemicals, ether is also used as an anesthetic, use a large amount of acetone and ether to precipitate brain protein first It is produced into brain protein powder. This step is very dangerous and must be produced in an explosion-proof workshop. The operation is cumbersome, pollutes the environment, and endangers the health of production operators.

Images