Kit for auxiliary diagnosis of multiple tumors by taking micro ribonucleic acid (RNA) combination as tumor marker, and detection method of kit
A tumor marker and auxiliary diagnosis technology, which is used in the determination/examination of microorganisms, biochemical equipment and methods, etc., can solve the problem that the non-invasive level cannot be achieved, the detection cannot be monitored in real time, and the early and accurate detection is mostly in the middle and late stages. Auxiliary diagnosis, etc.
A tumor marker and auxiliary diagnosis technology, which is used in the determination/examination of microorganisms, biochemical equipment and methods, etc., can solve the problem that the non-invasive level cannot be achieved, the detection cannot be monitored in real time, and the early and accurate detection is mostly in the middle and late stages. Auxiliary diagnosis, etc.
CN105950768APending Publication Date: 2016-09-21JIANGSU YINUOWAN CELL CLINIC CO LTD
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[0056] Below with regard to the specific embodiments, the present invention is further illustrated. It should be understood that these embodiments are only used to illustrate the present invention and are not intended to limit the scope of the present invention. After reading the present invention, those skilled in the art will understand the various equivalent forms of the present invention All modifications fall within the scope defined by the appended claims of the present application.
[0057] In order to understand the present invention, the present invention will be further described in detail below.
[0058] 1. The microRNA biomarkers and the tumors detected thereof include:
[0059] (1), miR-21-5p, miR-141-3p, miR-221-3p, miR-375 are used to detect prostate cancer;
[0060] (2), miR-21-5p, miR-122-5p, miR-192-5p, miR-223-3p are used to detect liver cancer;
[0061] (3), miR-17-3p, miR-92a-3p, miR-29a-3p are used to detect colorectal cancer;
[0062] (4), miR-155-5p,...
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The invention relates to a kit for auxiliary diagnosis of multiple tumors by taking a micro ribonucleic acid (RNA) combination as a tumor marker, and a detection method of the kit. The invention belongs to the fields of genetic engineering and oncology. The detection method comprises the steps of (1) extracting RNA from serum; (2) carrying out polyadenylation on the 3' end of micro RNA; (3) purifying and recovering a tailing product; (4) carrying out an inverse transcription reaction on the tailing product; (5) testing the relative expression of the micro RNA by means of a fluorescent quantitative PCR (polymerase chain reaction); (6) analyzing the result of the fluorescent quantitative PCR. The kit has the characteristics of high conservative property, space-time specificity, stability, tissue specificity and the like, and plays an important role in the aspects such as disease pathogenesis research, early diagnosis, individualized treatment and prognosis; furthermore, the kit is simple to operate, convenient in material obtaining, safe and noninvasive, and is conveniently used for screening of a great deal of diseases.
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technical field [0001] The invention belongs to the fields of genetic engineering and oncology, and relates to serum microRNA markers related to various human tumors and applications thereof. Background technique [0002] "Talking about the discoloration of cancer" is the number one attitude of most people towards cancer; cancer has always been a chronic disease that threatens human health and even life; on January 25, 2016, the world's top magazine in the field of cancer "CA: CA Cancer J Clin published online the 2015 cancer statistics released by the National Cancer Center, which shows that in 2015, there are expected to be 4.292 million new cancer cases and 2.814 million deaths in China, equivalent to an average of 12,000 per day. New cancer cases and 7,500 deaths from cancer; in view of this, we urgently need to explore new methods of tumor diagnosis and treatment. [0003] microRNA, also known as micro ribonucleic acid, is a kind of non-coding single-stranded small rib...
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Patent Timeline
21 Sep 2016
Publication
CN105950768A
- IPC
- C12Q1/68
- CPC
- C12Q1/6886; C12Q1/6806; C12Q1/6851; C12Q2600/158; C12Q2600/166; C12Q2600/178; C12Q2521/107; C12Q2563/107
- Inventors
- 曾骥孟; 蒙明慧