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Tamarix hispida COL (Constans-like) transcription factor encoding gene and application thereof

A technology of tamarisk tamarisk and transcription factor, which is applied in the fields of application, genetic engineering, plant genetic improvement, etc.

Active Publication Date: 2020-04-03
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem that it is impossible to use the COL transcription factor coding gene of Tamarix bristles to breed woody plants with excellent salt tolerance, the present invention provides the COL transcription factor coding gene of Tamarix bristles and its application

Method used

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  • Tamarix hispida COL (Constans-like) transcription factor encoding gene and application thereof
  • Tamarix hispida COL (Constans-like) transcription factor encoding gene and application thereof
  • Tamarix hispida COL (Constans-like) transcription factor encoding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Cloning of the gene encoding Tamarix bristles COL transcription factor

[0027] The seeds of Tamarix bristles were sown in the artificial soil prepared with peat soil and sand at a mass ratio of 2:1, placed at a relative humidity of 65-75% and a light intensity of 400 μmol m -2 ·s -1 , Grow in a greenhouse with an average temperature of 22±2°C. After 2 months of growth, use 0.3mol·L -1 NaHCO 3 The roots were irrigated with the solution for stress treatment, and the leaves and roots were taken at 0h, 12h, 24h and 48h respectively and put into liquid nitrogen for quick freezing for RNA extraction.

[0028] The CTAB method was used to extract the RNA of Tamarix seedlings in each stress treatment period, and the extracted RNA samples were sent to Shenzhen Huada Gene Technology Co., Ltd. for the construction and sequencing of the transcriptome library. The obtained RNA sequencing results of each processing period were compared and spliced, and the results after...

Embodiment 2

[0032] Example 2 Using bioinformatics software and online network resources to carry out sequence analysis on the gene encoding the transcription factor of Tamarix brixensis COL

[0033] As shown in SEQ ID No: 1, the length of the coding region of the gene coding for the transcription factor of Tamarix birch COL cloned in Example 1 is 1296 bp. It encodes 431 amino acids as shown in SEQ ID NO:2.

[0034] Utilize URL at ( http: / / au.expasy.org / tools / protparam.html ) ProtParam software to calculate and deduce the molecular weight and theoretical isoelectric point of the protein encoded by the gene encoding the COL transcription factor of Tamarix biriba.

[0035] The similarity analysis of the gene encoding the transcription factor COL of Tamarix birifolia was carried out through the BLASTx database at the URL (http: / / blast.ncbi.nlm.nih.gov / Blast.cgi?PROGRAM=blastx&BLAST_PROG RAMS=blastx&PAGE_TYPE=BlastSearch&SHOW_DEFAULTS=on&LINK_LOC=blasthome) , the comparison result is as fi...

Embodiment 3

[0046] Example 3 Analysis of expression patterns of ThCOL gene under different abiotic stresses and hormone treatments

[0047] ThCOL transcription factors play an important role in the regulation of plant stress resistance. A single Tamarix ThCOL gene with a complete open reading frame was cloned, and its sequence was analyzed. In order to study the function of the Tamarix ThCOL gene, real-time fluorescent quantitative RT-PCR was used to analyze its function under different abiotic stresses: high Salt - 400mmol NaCl, Drought - 20% PEG, Heavy Metal - 150μmol CdCl 2 and hormone stress: ABA-100μmol, GA 3 Expression profiles under -50 μmol treatment condition.

[0048] image 3 Shown is the expression pattern analysis of Tamarix bristle ThCOL protein under different abiotic stresses and hormone treatments. The results show that under three abiotic stresses and two hormone treatments, the expression of ThCOL in the roots and leaves of Tamarix at least one The stress treatment ...

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Abstract

The invention relates to a tamarix hispida COL (Constans-like) transcription factor encoding gene and application thereof, and belongs to the technical field of plant genetic engineering breeding. Inorder to solve the problem that a woody plant with good salt resistance can not be cultured through an existing tamarix hispida COL transcription factor encoding gene, the invention provides the tamarix hispida COL transcription factor encoding gene and the application thereof. The tamarix hispida COL transcription factor encoding gene is characterized in that the whole length of cDNA is 1296bp, and an amino acid sequence encoded by a cDNA sequence includes 431 amino acids which can be used for culturing salt-resistant transgenic plant varieties. An overexpression and suppression expression vector of the ThCOL gene is constructed, and transgenic tamarix hispida is obtained through instantaneous infection. Through experiments, including histochemistry staining, physiological index measurement and the like, a tamarix hispida ThCOL gene overexpression strain is discovered to own obvious salt resistance, and therefore, the tamarix hispida COL transcription factor encoding gene is a good gene used for forest salt resistance genetic engineering breeding.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering and breeding, and in particular relates to a gene coding for a COL transcription factor of tamarisk bristles and an application thereof. Background technique [0002] Some abiotic stresses such as salinity, low temperature and drought can cause a series of morphological, physiological, biochemical and molecular changes in plants, which seriously affect the growth and development of plants, and even lead to plant death. In order to adapt to the surrounding environment, when plants are subjected to high-salt, low-temperature, drought and other stresses, cells quickly perceive external stress signals through a series of signal transmission processes, generate stress signals and collect them in the nucleus, and finally activate specific transcriptional regulators. Transcription factors enter the nucleus through functional regions, directly bind to cis-acting elements in specific gene...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/82C12N1/21C07K14/415A01H5/00A01H6/00C12R1/01C12R1/19
CPCC07K14/415C12N15/8273
Inventor 高彩球雷晓锦刘中原谭冰王春瑶房家茹
Owner NORTHEAST FORESTRY UNIVERSITY
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