Non-isotonic preserving fluid for liquid-based thin-layer exfoliative cells
A technology of exfoliated cells and preservation solution, which is applied in the field of antibacterial and antiseptic preservation solutions, which can solve the problems of failure to fix cells, loss of acid-base buffering capacity of liquid-based thin-layer exfoliated cell preservation solution, and weakening of buffering capacity of acid-base buffer system, etc. problem, to achieve the effect of clear background of microscopic examination and increase detection rate
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Embodiment 1
[0040] An anisotonic preservation solution for liquid-based thin-layer exfoliated cells, comprising the following components by weight: 10 parts of formaldehyde, 15 parts of ethanol, 9.2 parts of glycine, 3.2 parts of urea, 2 parts of procaine hydrochloride, 1.5 parts of propylene glycol, 1.5 parts of dimethyl sulfoxide, 1 part of sodium phosphate, 3.5 parts of trypsin, 0.2 parts of acetic acid, 1.09 parts of disodium hydrogen phosphate, 0.31 parts of sodium dihydrogen phosphate, and 51.5 parts of water. The preparation method is as follows: the raw materials are fed into a glass container, fully dissolved and mixed evenly, and then filtered with a 0.1 μm filter membrane to obtain a filtrate, which is sealed in a plastic or glass container for use.
Embodiment 2
[0042] An anisotonic preservation solution for liquid-based thin-layer exfoliated cells, comprising the following components by weight: 9.5 parts of formaldehyde, 15.3 parts of ethanol, 9.1 parts of glycine, 3.1 parts of urea, 1.9 parts of procaine hydrochloride, 1.4 parts of propylene glycol, 1.6 parts of dimethyl sulfoxide, 1.1 parts of sodium phosphate, 3.6 parts of trypsin, 0.1 part of acetic acid, 1 part of disodium hydrogen phosphate, 0.3 part of sodium dihydrogen phosphate, and 52 parts of water. The preparation method is as follows: the raw materials are fed into a glass container, fully dissolved and mixed evenly, and then filtered with a 0.1 μm filter membrane to obtain a filtrate, which is sealed in a plastic or glass container for use.
Embodiment 3
[0044] An isotonic preservation solution for liquid-based thin-layer exfoliated cells, comprising the following components by weight: 10.6 parts of formaldehyde, 14.7 parts of ethanol, 9.4 parts of glycine, 3.3 parts of urea, 2.1 parts of procaine hydrochloride, 1.35 parts of propylene glycol, 1.4 parts of dimethyl sulfoxide, 0.9 parts of sodium phosphate, 3.4 parts of trypsin, 0.25 parts of acetic acid, 1.1 parts of disodium hydrogen phosphate, 0.32 parts of sodium dihydrogen phosphate, and 51.18 parts of water. The preparation method is as follows: the raw materials are fed into a glass container, fully dissolved and mixed evenly, and then filtered with a 0.1 μm filter membrane to obtain a filtrate, which is sealed in a plastic or glass container for use.
[0045] Measurement method: The density, osmotic pressure and pH value of the anisotonic preservation solution for liquid-based thin-layer exfoliated cells prepared in Examples 1-3 were respectively determined according to ...
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