Human stomache cancer endothelial-cell specific combination short peptide series

A vascular endothelial-specific binding technology, applied in the field of biomedicine, can solve the problems of low expression of heterogeneous molecules, difficult cultivation, and undiscovered specific molecules of gastric cancer vascular endothelial cells.

Inactive Publication Date: 2005-12-21
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

Due to the difficulty in culturing gastric cancer microvascular endothelial cells in vitro, the expression level of heterogeneous molecules is low, and it is difficult to detect, isolate and purify by conventional methods, so no clear gastric cancer vascular endothelial cell-specific molecules have been found so far.

Method used

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  • Human stomache cancer endothelial-cell specific combination short peptide series
  • Human stomache cancer endothelial-cell specific combination short peptide series
  • Human stomache cancer endothelial-cell specific combination short peptide series

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Embodiment Construction

[0036] The molecular markers on the surface of gastric cancer vascular endothelial cells are the key targets for the treatment of gastric cancer vascular inhibition. The applicant successfully obtained a group of cyclic short peptides GEBPs that specifically bind to human gastric cancer vascular endothelial cells through in vitro whole-cell screening of phage random peptide libraries. The binding specificity of GEBPs was identified by phage cell binding assay, immunocytochemical / histochemical staining, cell binding competition inhibition assay, and immunofluorescence detection.

[0037] The specific method is as follows:

[0038] 1. Establishment of in vitro culture model of human gastric cancer vascular endothelial cells

[0039] 1.1. Primary isolation and culture of HUVECs

[0040] The method of Jaffe et al. was modified to isolate and culture primary human umbilical vein endothelial cells (HUVECs). The umbilical cords of newborns were obtained from the Obstetrics and Gynec...

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Abstract

This invention belongs to the biomedicine field, involves screening phage peptide library in vitro to get GEBPs. This invention utilizes Transwell co culturing technology to set up culture in vitro models of endothelial cell of stomach cancer blood vessel, through screening phage peptide library in vitro, get GEBPs. The peptides have certain theory meaning and great potential applying value to the research such as stomach cancer diagnosis, blood vessel targeted cure, etc.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a series of short peptide GEBPs specifically bound to human gastric cancer vascular endothelial cells obtained by in vitro screening of a phage peptide library. Background technique [0002] Gastric cancer is a common malignant tumor. Traditional treatment methods such as surgery, chemotherapy and radiotherapy cannot achieve ideal therapeutic effects. Studies have found that angiogenesis is one of the important conditions for the growth and metastasis of solid tumors, and tumor angiogenesis inhibitory therapy has become a hot spot in the field of tumor treatment research, and has shown a good therapeutic prospect. However, some blood vessel inhibitors entering clinical trials are difficult to achieve the tumor suppressing effects shown in animal experiments, and the clinical effects are not satisfactory. The reason is that one of the most pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/64
Inventor 梁树辉丁杰林涛潘阳林吴开春樊代明
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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