Cold-resistant anthurium transgenic culturing method

A cultivation method and transgenic technology, applied in the field of transgenic cultivation of cold-resistant anthurium, can solve the problems of increasing production cost, reducing ornamental and commercial quality of anthurium, and achieve the effects of improving quality, improving ornamental and commercial quality, and reducing production cost

Inactive Publication Date: 2010-02-10
ZHEJIANG UNIV
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  • Abstract
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Problems solved by technology

At the same time, due to the low temperature in winter in our country, it is difficult to plant anthurium in the natural environment of anthurium in our country, and it is necessary to control t

Method used

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Embodiment 1

[0029] The terminal buds of the Anthurium variety "Huayan" were used as explants, inoculated into the induction medium to induce callus; the callus induced for 2 weeks was taken, transferred to the subculture medium, and subcultured to the callus Enter the rapid proliferation phase; take the fast-proliferating callus and pre-culture the recipient callus for 3 days under the dark condition of 25°C; take the pre-cultured callus (about 2000 petri dishes, containing more than 20000 Injured tissue), soaked in the Agrobacterium suspension for 15 minutes, then discarded the bacterial solution, blotted dry with sterile filter paper, inoculated on the co-culture medium, and co-cultivated in the dark at 23°C for 3 days; the co-cultured callus After the tissue was washed and dried, it was transferred to the selection medium, and cultured in the dark at 25°C for 4 weeks, and the grown resistant calli (about 300 pieces) were collected, transferred to a new selection medium, and then Three ...

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Abstract

The invention relates to a cold-resistant anthurium transgenic culturing method which comprises the following steps of: selecting apical buds of a main-bred anthurium variety as explants; inducing calli; subculturing the calli till a fast proliferation period; carrying out 4 turns of selection by transferring the calli into a selection culture medium; selecting the resistant calli for induction and differentiation so as to obtain seedlings; carrying out histochemical staining and molecular detection on resistant plants; identifying the differentiated seedlings containing target genes; transferring the differentiated seedlings into a rooting culture medium so that the differentiated seedlings can grow into normal seedlings; determining cold resistance of selected transgenic plants; remaining the transgenic plants which are free from cold damage and grow well; continuing to grow the selected transgenic anthurium plants; assessing and verifying the expression stability of the cold resistance; carrying out proliferation on the stable and fine plants; and culturing the transgenic cold-resistant anthurium which resists the cold damage at low temperatures.

Description

technical field [0001] The invention relates to a method for transgenic breeding of cold-resistant anthurium. Background technique [0002] Anthurium is a perennial herbaceous plant of the family Araceae Anthurium, which is listed as the main cut flower species in many tropical countries and regions, and is the second largest tropical flower commodity after Tropical Orchid. Anthurium is both one of the 10 most popular cut flowers and one of the 10 most popular potted plants. Its sales are second only to Phalaenopsis and are the fastest growing of all cut flower varieties. [0003] my country's anthurium industry has experienced more than 20 years of development, and has made great progress in terms of production scale and research and development capabilities, but the overall research and development and sales are far from the international level. Although it is produced in many places, there is still no new variety of anthurium with independent intellectual property rights...

Claims

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Application Information

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IPC IPC(8): C12N15/82A01H4/00
Inventor 田丹青舒小丽葛亚英叶红霞周志丹吴殿星
Owner ZHEJIANG UNIV
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