Method for cultivating rice with high resistant starch content
A technology of high resistant starch and cultivation method, applied in the field of cultivation of high resistant starch content rice, can solve problems such as affecting the quality of life, affecting the survival and development of human society, etc.
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[0032] The mature embryos of the rice variety "Zhehui 7954" with an amylose content of 24.5% were used as explants, inoculated into the induction medium, and induced to form callus under the conditions of 25±1°C and light intensity of 3000 lux;
[0033] Take the callus induced to form for 2 weeks, transfer to the subculture medium, and subculture until the callus enters the doubling phase;
[0034] Take the doubly-proliferated callus, and pre-culture the recipient callus for 2 days at 25±1°C and under dark conditions protected from light;
[0035] Take the pre-cultured callus (about 300 petri dishes, containing more than 1500 pieces of callus), soak in the Agrobacterium suspension for 10 minutes, then discard the bacteria solution, dry it with sterile filter paper, and inoculate in On the co-culture medium, co-cultivate for 2 days at 25±1°C in dark conditions protected from light;
[0036] Take the above-mentioned co-cultured callus, wash and dry it, transfer it to the screen...
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