Method for cultivating rice with high resistant starch content

A technology of high resistant starch and cultivation method, applied in the field of cultivation of high resistant starch content rice, can solve problems such as affecting the quality of life, affecting the survival and development of human society, etc.

Inactive Publication Date: 2012-07-11
ZHEJIANG UNIV
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Problems solved by technology

[0003] Diabetes and other chronic diseases of the blood system are showing rapid growth, spreading to rural areas, and younger ages, which are increasingly affecting the survival and development of human society (the number one killer of life, seriously affecting the quality of life, and annual medical expenses. up to 100 billion yuan), and there is currently no cure

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  • Method for cultivating rice with high resistant starch content

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Embodiment 1

[0032] The mature embryos of the rice variety "Zhehui 7954" with an amylose content of 24.5% were used as explants, inoculated into the induction medium, and induced to form callus under the conditions of 25±1°C and light intensity of 3000 lux;

[0033] Take the callus induced to form for 2 weeks, transfer to the subculture medium, and subculture until the callus enters the doubling phase;

[0034] Take the doubly-proliferated callus, and pre-culture the recipient callus for 2 days at 25±1°C and under dark conditions protected from light;

[0035] Take the pre-cultured callus (about 300 petri dishes, containing more than 1500 pieces of callus), soak in the Agrobacterium suspension for 10 minutes, then discard the bacteria solution, dry it with sterile filter paper, and inoculate in On the co-culture medium, co-cultivate for 2 days at 25±1°C in dark conditions protected from light;

[0036] Take the above-mentioned co-cultured callus, wash and dry it, transfer it to the screen...

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Abstract

The invention relates to a method for cultivating rice with high resistant starch content, which comprises the steps: using mature embryos of a rice variety with 20%-25% of amylase content as explants to induce callus and to be sub-cultured till a multiplied proliferation period, performing pre-cultivation according to conditions, performing agrobacterium tumefaciens co-culture conversion, transferring converted mature embryos to a screening culture medium to be screened for three times, using resistant callus to induce and differentiate mature embryos into seedlings, performing marker gene histochemical staining and screened gene molecule detection to resistant plants, identifying differentiated seedlings inserted sequences and containing target genes ISA1, detecting the resistant starch content of selected transgenic plants, selecting and remaining transgenic plants with more than 10% of resistant starch content, continuing to plant and evaluating the expression stability of the resistant starch content, cultivating excellent plants with more than 10% of resistant starch content and finally cultivating the rice with high resistant starch content. The rice with high resistant starch content can be used for development of products special for diabetes and has important significance on implementation of dietotherapy of persons with diabetes.

Description

technical field [0001] The invention relates to a method for cultivating rice with high resistant starch content. Background technique [0002] According to statistics from the Ministry of Health, there were about 92 million people with diabetes in my country in 2010, accounting for 9.3% of the total population in my country, and 142 million people with low glucose tolerance. [0003] Diabetes and other chronic diseases of the blood system are showing rapid growth, spreading to rural areas, and younger ages, which are increasingly affecting the survival and development of human society (the number one killer of life, seriously affecting the quality of life, and annual medical expenses. up to 100 billion yuan), and there is currently no cure. [0004] Medical research shows that: In addition to genetic factors, chronic diseases of the blood system such as diabetes are mainly caused by daily living habits such as daily diet, and a high-sugar, high-fat, and high-protein diet i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 吴殿星舒小丽张宁徐开盛
Owner ZHEJIANG UNIV
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