Feed additive composition

Inactive Publication Date: 2014-08-21
DUPONT NUTRITION BIOSCIENCES APS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0005]A seminal finding of the present invention is that a DFM in combination with a protease, x

Problems solved by technology

Although combinations of DFMs with some enzymes have been contemplated,

Method used

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  • Feed additive composition
  • Feed additive composition

Examples

Experimental program
Comparison scheme
Effect test

example 1

Materials and Methods

[0440]Three thousand six hundred one-day-old Cobb male chicks were purchased from a commercial hatchery. At study initiation, fifty males were allocated to each treatment pen by blocks. The study consisted of the following treatments (Table 1):

TABLE 1Experimental design of Example 1.ClostridiumperfringensAdditional TreatmentChallengePhytase1enzyme2DFM31No500 FTU / kgNoneNone2Yes500 FTU / kgNoneNone3Yes500 FTU / kgAmylase None(200 u / kg) 4Yes500 FTU / kgProtease None(5000 u / kg)5Yes500 FTU / kgXylanase4 None(2000 u / kg)Amylase4 (200 u / kg) Protease4 (5000 u / kg)6Yes500 FTU / kgNoneEnviva Pro(7.5 × 104 CFU / g)7Yes500 FTU / kgAmylase Enviva Pro(200 u / kg) (7.5 × 104 CFU / g)8Yes500 FTU / kgProtease Enviva Pro(5000 u / kg)(7.5 × 104 CFU / g)9Yes500 FTU / kgXylanase4 Enviva Pro(2000 u / kg)(7.5 × 104 CFU / g)Amylase4 (200 u / kg) Protease4 (5000 u / kg)1Phytase from E.coli.2Amylase from Bacilluslicheniformis, xylanase from Trichodermareesei, protease from Bacillussubtilis.3Enviva Pro ® is combination of B...

example 2

Materials and Methods

[0451]Cobb 500 male broiler chicks were obtained from a commercial hatchery. A total of 26 chicks were randomly assigned to one of 8 replicate pens per treatment. Floor pens (16 ft2 / pen) were located in a curtain-sided house containing controlled heating, circulating fans, heat lamps and fresh wood shavings. Birds were exposed to fluorescent lighting in a 24 h light cycle for the first four days and then 16 light:8 hour dark cycle for the remainder of the experiment. Feed was provided in bell feeders and water supplied via nipple drinkers ad libitum. A 5× dose of Coccivac-B (Intervet) was administered manually with a syringe into the oral cavity of chicks at one day of age.

TABLE 3Experimental design of Example 2.CoccidiosisAdditional TreatmentvaccinePhytase1enzyme2DFM315X500 FTU / kgNoneNone25X500 FTU / kgNoneEnviva Pro(7.5 × 104 CFU / g)35X500 FTU / kgXylanase4 None(1000 u / kg)Amylase4 (1800 u / kg)Protease4 (5000 u / kg)45X500 FTU / kgXylanase4 Enviva Pro(1000 u / kg)(7.5 × 10...

example 3

Materials and Methods

[0459]One digestibility trial with broiler chickens was conducted to determine the effects of dietary enzymes and DFMs treatments on nutrient utilisation. The cages were housed in environmentally controlled rooms. The birds received 20-hour fluorescent illumination and, allowed free access to the diets and water. On day 1, a broiler live coccidiosis vaccine was given to all chicks via drinking water. Paper was provided on cage wire-floor for the first three days to enable recycling of Eimeria Oocystes. The study consisted of the following treatments (Table 5).

TABLE 5Experimental design of Example 3.TreatmentPhytase1Additional enzyme2DFM31500 FTU / kgNoneNone2500 FTU / kgXylanase4 (1000 u / kg)NoneAmylase 14 (1800 u / kg)Protease4 (5000 u / kg)3500 FTU / kgXylanase (2000 u / kg)NoneAmylase 2 (200 u / kg)4500 FTU / kgXylanase5 (2000 u / kg)NoneAmylase 25 (200 u / kg)Protease5 (5000 u / kg)5500 FTU / kgNoneEnviva Pro(7.5 × 104 CFU / g)6500 FTU / kgXylanase4 (1000 u / kg)Enviva ProAmylase 14 (1800...

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PUM

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Abstract

A feed additive composition comprising a direct fed microbial in combination with a protease, a xylanase, an amylase and a phytase, and a method for improving the performance of a subject or for improving digestibility of a raw material in a feed (e.g. nutrient digestibility, such as amino acid digestibility), or for improving nitrogen retention, or for avoiding the negative effects of necrotic enteritis or for improving feed conversion ratio (FCR) or for improving weight gain in a subject or for improving feed efficiency in a subject or for modulating (e.g. improving) the immune response of the subject or for promoting the growth of beneficial bacteria in the gastrointestinal tract of a subject, which method comprising administering to a subject a direct fed microbial in combination with a protease, a xylanase, an amylase and a phytase.

Description

FIELD OF INVENTION[0001]The present invention relates to methods for improving feed compositions using a direct fed microbial in combination with a specific combination of enzymes, and to a feed additive composition comprising a direct fed microbial in combination with a specific combination of enzymes. The present invention further relates to uses and kits.BACKGROUND OF THE INVENTION[0002]Supplemental enzymes are used as additives to animal feed, particularly poultry and swine feeds, as a means to improve nutrient utilization and production performance characteristics. Enzyme blends are available to improve the nutritional value of diets containing soybean meal, animal protein meals, or high fibre food by-products.[0003]The concept of direct fed microbials (DFM) involves the feeding of beneficial microbes to animals, such as dairy cattle when they are under periods of stress (disease, ration changes, environmental or production challenges). Probiotics is another term for this categ...

Claims

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Application Information

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IPC IPC(8): A61K35/74A61K38/46A61K38/48A61K38/47A23K1/165A23K1/00
CPCA61K35/741A23K1/1653A23K1/009A61K35/742C12Y302/01A61K38/47A61K38/465C12Y304/00C12Y301/03A61K38/48A23K10/18A23K20/189A23K40/10A23K40/30A23K50/75C12N1/20C12N1/205C12R2001/125A61P1/04A61P33/02A61P43/00
Inventor MILLAN, LUIS FERNANDO ROMERO
Owner DUPONT NUTRITION BIOSCIENCES APS
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