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Method for preparing liposome enveloped by catechin

A catechin and liposome technology, applied in the chemical field, can solve the problems of low bioavailability and low bioavailability, and achieve the effect of improving antioxidant capacity and stability

Inactive Publication Date: 2012-03-28
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because catechins have strong antioxidant properties, they are prone to reactions such as oxidation, polymerization, and condensation under conditions such as humidity, sunlight, and high temperature, and their bioavailability is not high in an unprotected state. Taking advantage of the problems, people hope to prepare catechin liposomes to embed catechin, improve its antioxidant capacity, and solve the problem of its low bioavailability

Method used

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  • Method for preparing liposome enveloped by catechin
  • Method for preparing liposome enveloped by catechin
  • Method for preparing liposome enveloped by catechin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1, a kind of preparation method of the liposome of encapsulating catechin, carries out following steps successively:

[0027] 1) Dissolve 45mg of soybean lecithin and 11.6mg of cholesterol in 9ml of chloroform to fully dissolve it (soy lecithin and cholesterol). Evaporate on the instrument for 20 minutes to form a lipid film;

[0028] 2), add 9ml of ether to the lipid film to dissolve the lipid film, then add 3ml of catechin-containing phosphate buffer solution, and ultrasonicate on a water bath ultrasonic instrument at 10°C (for example, in a 30kHz ultrasonic wave, ultrasonic 10 ~20min) to make the mixture into a stable single-phase system.

[0029] The catechin-containing phosphate buffer solution was prepared according to the ratio of dissolving 10 mg of catechin in 1 ml of PBS phosphate buffer solution (pH 6.81).

[0030] 3) The single-phase system was decompressed to 0.07MPa at room temperature and evaporated for 10 minutes to form 6ml of aqueous suspe...

Embodiment 2

[0048] Embodiment 2, a kind of preparation method of the liposome of encapsulating catechin, carries out following steps successively:

[0049] 1), take 22.5mg soybean lecithin and 12mg cholesterol and dissolve in 9ml dichloromethane, make it (soy lecithin and cholesterol) fully dissolve, in the water bath of 35 ℃, vacuum pressure is under 0.035MPa, in rotary evaporation Evaporate on the instrument for 30 minutes to form a lipid film;

[0050] 2) Add 9ml of ether to the lipid film to dissolve the lipid film, then add 3ml of catechin-containing phosphate buffer solution, and sonicate on a water bath ultrasonicator at 10°C until the mixture becomes a stable single-phase system.

[0051] The catechin-containing phosphate buffer solution was prepared according to the ratio of 12 mg catechin dissolved in 1 ml PBS phosphate buffer solution (pH 6.81).

[0052] 3) The single-phase system was decompressed to 0.05 MPa at room temperature and evaporated for 15 minutes until 6 ml of aque...

Embodiment 3

[0059] Embodiment 3, a kind of preparation method of the liposome of encapsulating catechin, carries out following steps successively:

[0060] 1) Dissolve 67.5mg of soybean lecithin and 11.6mg of cholesterol in 9ml of dichloromethane to fully dissolve it (soy lecithin and cholesterol). Evaporate on the evaporator for 10 minutes to form a lipid film;

[0061] 2) Add 9ml of ether to the lipid film to dissolve the lipid film, then add 3ml of catechin-containing phosphate buffer solution, and sonicate on a water bath ultrasonicator at 10°C until the mixture becomes a stable single-phase system.

[0062] The catechin-containing phosphate buffer solution was prepared according to the ratio of 14 mg catechin dissolved in 1 ml of PBS phosphate buffer solution (pH 6.81).

[0063] 3) The single-phase system was decompressed to 0.03 MPa at room temperature and evaporated for 15 minutes until 6 ml of aqueous suspension was formed to obtain catechin-encapsulated liposomes.

[0064] In t...

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Abstract

The invention discloses a method for preparing liposome enveloped by catechin. The method sequentially comprises the following steps of: 1) mixing soya bean lecithin and cholesterol in a mass ratio of (1.5-6):1, and dissolving in a solvent I or dissolving soya bean lecithin in the solvent I; evaporating in water bath at the temperature of between 30 and 40 DEG C under reduced pressure for 10 to 30 minutes to form a layer of thin lipid membrane; 2) adding a solvent II into the thin lipid film to dissolve the thin lipid membrane, adding a phosphate buffer solution containing catechin, and uniformly mixing to form a stable single-phase system; and 3) decompressing the single-phase system and evaporating for 30 to 50 minutes to form water based suspension and thus obtaining the liposome enveloped by the catechin. The obtained liposome enveloped by the catechin can improve the oxidation resistance of catechin, so that the bioavailability of the catechin can be improved.

Description

technical field [0001] The invention relates to catechins and phospholipids in the field of chemistry, in particular to liposomes encapsulating catechins and a preparation method thereof. Background technique [0002] Liposome is a multilamellar closed vesicle structure composed of lipid components such as phospholipids and cholesterol. Liposomes can be divided into two types: single-lamellar liposomes and multi-lamellar liposomes. Liposomes composed of natural ingredients are bilayer structures, similar to biological cell membranes, so they are also called artificial biological membranes. Phospholipids in liposomes are amphiphilic molecules, which can combine with water-soluble substances and fat-soluble substances. Lipid-soluble substances are encapsulated in the bilayer membrane of the vesicle. [0003] The substances that constitute the liposome bilayer mainly include phospholipids (natural phospholipids, synthetic phospholipids) and cholesterol. Phospholipids are lip...

Claims

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Application Information

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IPC IPC(8): A23L3/3544A23B4/20
Inventor 关荣发方建军蒋家新刘明启马洁清金慧伟
Owner CHINA JILIANG UNIV
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