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Preparation method of stem cell secreted factor flexible liposome

A technology of flexible liposomes and secretion factors, which is applied in the direction of liposome delivery, medical preparations containing non-active ingredients, medical preparations containing active ingredients, etc., can solve the problems of good curative effect, and achieve good adhesion and Effects of absorption, reduction of adverse reactions, and enhancement of penetration ability

Inactive Publication Date: 2018-11-30
傅松涛
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, there are only clinical reports on the application of a single cytokine in scar repair, and the curative effect is very good

Method used

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  • Preparation method of stem cell secreted factor flexible liposome
  • Preparation method of stem cell secreted factor flexible liposome
  • Preparation method of stem cell secreted factor flexible liposome

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Step 1, primary isolation, purification, culture and identification of stem cells: cut the fresh umbilical cord into 1-2cm long sections, cut open the fetal membranes, remove the veins and two arteries, then extract the jelly-like substance, and cut into 1-2mm3 sized sections Tissue blocks were inoculated in cell culture dishes, and complete medium was added after half an hour, placed in a cell culture incubator at 37°C and 5% CO2 for culture, and the medium was changed after 3 days. After 10 days, under an inverted microscope, mesenchymal stem cells could be seen crawling out from around the tissue. When the cells were cultured to the third passage, the umbilical cord mesenchymal stem cells were purified, and the stemness of the cells was identified: ①morphological identification: the mesenchymal stem cells were spindle-shaped adherent cells; ②surface marker identification: the mesenchymal stem cells were high Expression of CD29, CD44, CD105, low expression of CD45, CD...

Embodiment 2

[0044] In step three, 10ml of liposome preparation is used, and the formula ratio is: phospholipid 0.150g, cholesterol 0.030g, stem cell secreted factor concentrate 6mL, vitamin E 0.3mg, vitamin C 0.3mg. Other steps are identical with embodiment 1.

Embodiment 3

[0046] In step 3, 10ml of liposome preparation is used, and the formula ratio is: phospholipid 0.120g, cholesterol 0.025g, stem cell secreted factor concentrate 6mL, vitamin E 0.25mg, vitamin C 0.25mg. Other steps are identical with embodiment 1.

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Abstract

The invention relates to a preparation method of a stem cell secreted factor flexible liposome. The preparation method comprises the steps that 0.100-0.150g of phospholipid, 0.020-0.030g of cholesterol, 0.2-0.3mg of vitamin E are dissolved by using 10ml of organic solvent, decompression and rotary evaporation are performed to remove the organic solvent and form a lipid film, a stem cell secreted factor solution is hydrated with the lipid film to form a stem cell secreted factor flexible liposome solution, the obtained stem cell secreted factor flexible liposome solution is subjected to homogenization treatment, and the stem cell secreted factor flexible liposome is obtained. The preparation method of the stem cell secreted factor flexible liposome effectively improves the retention time ofmesenchymal stem cell secreted factors, facilitates the adhesion and absorption of drugs and the skin, reduces stimulation and adverse effects, enhances the penetration ability to reach deep tissue,enhances the curative effects of secreted factors of mesenchymal stem cells, improves the utilization degree of the mesenchymal stem cells, and has no toxic side effects.

Description

technical field [0001] The invention relates to the field of liposomes with medical effects, in particular to a method for preparing stem cell secreted factor flexible liposomes. Background technique [0002] Wound repair is an extremely complex biological process, which includes cellular, immunological, and molecular biological processes in which various repair cells, cytokines, and many other factors participate. The process of wound repair includes the stages of granulation tissue formation, collagen filling, wound contraction, epithelial covering, and collagen remodeling. Scars are an inevitable byproduct of the wound healing process. However, pathological scar formation caused by over-repair will cause appearance damage and functional impairment to varying degrees. Pathological scars mainly include hypertrophic scars and keloids, histologically characterized by a large number of fibroblast proliferation, excessive deposition of collagen, proteoglycan, and glycoprotein...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61K9/127A61K47/24A61P17/02A61K8/99A61K8/55A61Q19/00
CPCA61K8/553A61K8/99A61K9/127A61K35/28A61K47/24A61Q19/00A61P17/02
Inventor 傅松涛解军刘美林
Owner 傅松涛
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