34results about How to "Promote epithelialization" patented technology

Transplantation of epithelial stem cells, cultured ex vivo on specifically treated amniotic membrane, yields, with that amniotic membrane, a surgical graft having expanded epithelial stem cells. The method of creating this graft and the graft itself provide simple and effective means to reconstruct damaged tissue, a preferred example being corneal tissue. The source of the epithelial stem cells can be a very small explant from healthy autologous and allogeneic tissue biopsy. The amniotic membrane is treated such that its extracellular matrix is maintained, but its cells are killed.

An occlusion device comprises a distal end, a proximal end and an elastic braided body which is provided between the proximal end and the distal end and made of wires. The elastic braided body is composed of a multi-stage braided net, which comprises at least a first-stage braided net that is closest to the distal end and made of a plurality of first-stage wires, and a second-stage braided net which is braided by a plurality of first-stage wires and second-stage wires all together. The minimum cross-section area of the first-stage braided net after being compressed toward the direction perpendicular to an axis of the elastic braided body is less than the minimum cross-section area of any other-stage braided net after being compressed toward the axis. A method for manufacturing the occlusion device is also provided.

The invention discloses a method for preparing a photo-crosslinking chitosan hydrogel film. Biocompatible macromolecular chitosan as an initiator is subjected to chemical modification and introduced with hydroxypropyl and nitrine radicals at the 6-O site and N site respectively to form water-soluble photo-crosslinking chitosan; and the water solution of the photo-crosslinking chitosan is irradiated by ultraviolet light to form chitosan hydrogel so as to obtain the photo-crosslinking chitosan hydrogel film. The photo-crosslinking chitosan hydrogel film formed by a chitosan hydrogel film through ultraviolet light crosslinking by a physical method avoids chemical crosslinking with biological toxicity, improves the biocompatibility of the chitosan hydrogel film, realizes the clinical application of the chitosan hydrogel film, and provides a new path for the clinical application of the chitosan hydrogel film.

The invention relates to a preparation method of a stem cell secreted factor flexible liposome. The preparation method comprises the steps that 0.100-0.150g of phospholipid, 0.020-0.030g of cholesterol, 0.2-0.3mg of vitamin E are dissolved by using 10ml of organic solvent, decompression and rotary evaporation are performed to remove the organic solvent and form a lipid film, a stem cell secreted factor solution is hydrated with the lipid film to form a stem cell secreted factor flexible liposome solution, the obtained stem cell secreted factor flexible liposome solution is subjected to homogenization treatment, and the stem cell secreted factor flexible liposome is obtained. The preparation method of the stem cell secreted factor flexible liposome effectively improves the retention time ofmesenchymal stem cell secreted factors, facilitates the adhesion and absorption of drugs and the skin, reduces stimulation and adverse effects, enhances the penetration ability to reach deep tissue,enhances the curative effects of secreted factors of mesenchymal stem cells, improves the utilization degree of the mesenchymal stem cells, and has no toxic side effects.

The invention relates to the technical field of tissue engineering and medical wound repairing, in particular to an amnion innate stem cell carried frozen active amnion particle and a conditioned medium and application thereof. Discarded fresh amnions are prepared into particles and then frozen into liquid nitrogen by means of serum-free stem cell freezing liquid; thus, complete matrix components of amnion are maintained while amnion epithelial cell activity is maintained for a long time, and further, disease transmission is avoided effectively. The conditioned medium which is prepared and collected by means of the frozen active amnion particles can promote chemotaxis and migration of human epidermal cells, fibroblast and endothelial cells effectively. In terms of zoografting, wound healing can be improved by multiple ways of regulating inflammatory reaction, promoting vascularization, quickening epithelization and the like through the active amnion particles; further, the amnion matrix can be directly used as a dermis equivalent to induce dermal regeneration so as to improve wound healing quality remarkably. The amnion innate stem cell carried frozen active amnion particle and the conditioned medium thereof can provide simple but effective ways for wound repairing.

The invention relates to a wound covering good in biocompatibility and capable of accelerating wound healing and a preparation method of the wound covering. The wound covering comprises a film sheet,and the film sheet is prepared from a carrier with good biocompatibility and high mechanical strength and protein containing dopa groups; the carrier is collagen, and the protein containing the dopa group is mussel mucin. The collagen is mixed with mussel mucin, a dry membrane is prepared by a semi-freeze-drying method under a low-temperature condition, the mussel mucin is immobilized on the collagen through chemical crosslinking, residual cross-linking agents are removed through rinsing, then the mussel mucin is stored in a preservation solution, the stability of the protein structure is enhanced, the activity of the collagen and the mussel mucin is maintained, and a collagen matrix has excellent mechanical strength and can be trimmed into any shape; tissue epithelialization can be accelerated, and wound healing is promoted; and the biocompatibility is good, the wound surface is not adhered, and secondary injury to the wound surface is avoided.

The invention provides a novel decellularized amnion preparation method, and relates to the technical field of biological materials. The method comprises ultrasonically treating the amnion and then physically scraping cells on the amnion to obtain the decellularized amnion. According to the method, the ultrasonic treatment and physical scraping mode is adopted to remove the cells on the surface ofthe decellularized amnion, the use of chemical reagents like trypsin is avoided, and the prepared decellularized amnion product is safer and reduces the residual chemical substances harmful to the body in the decellularized amnion. At the same time, the integrity of the amniotic matrix is retained to the utmost extent.

A hygienical comestic, especially a whitening cosmetic and its preparation method. It contains five materials of AíóBíóCíóD and E. A material comprises SS methyl gluoside, stearate, SSE-20 methyl gluoside, and addition compound of stearic acid and ethylene oxide, silicon oil DC-200 and son on. B material comprises CAD940,water, glycerol and so on. C material comprises TEA, water. D material comprises EGF, ATB-26000, STAY-C50. E material comprises essences.

The invention discloses a cracked nipple patch for women in a lactation period. The patch comprises a round hydrogel layer, a plastic protection film is arranged on the upper surface of the hydrogel layer, and a handle is arranged at the lower surface of the hydrogel layer. The hydrogel layer is made from the following components, by weight part, 15-20 parts of a seaweed active extract, 5-8 parts of collagen, 30-45 parts of an aloe extract, 3-5 parts of zinc chloride, 6-8 parts of vitamin B5, 3-5 parts of vitamin A, 1-3 parts of calcium lactate, 15-20 parts of agar and 15-20 parts of distilled water. The patch is excellent in one-way air permeability, can keep the area around a wound surface and a wound surface area in an environment with optimum humidity, is free of any toxic or side effect and can effectively prevent allergy. The patch is soft and free of adhesion, provides a long-lasting cool, refreshing and comfortable curative effect on the wound tissues, can alleviate pain, and can effectively promote the wound tissues to fast heal.

The invention relates to application of an eIF3I-PDL1-IRS4 axis in preparation of a medicine for treating refractory ulcer. It is found that: when PDL1 is used for treating wounds, tissue formation, inflammation fading and reduction in vascularity can be induced; epithelization is accelerated; inflammation is relieved; healing of diabetic ulcer (DU) wounds is helped; in a DU wound surface, the expression of IRS4 is increased; the expression of the IRS4 of HaCaT cells is knocked down; the proliferation and migration of the cells are increased; when the eIF3I is over-expressed in HaCaT cells, the proliferation and migration of the cells are remarkably increased; and inflammatory infiltration is relieved. The invention prompts that the targeted eIF3I-PDL1-IRS4 axis can be used as a novel treatment method for treating refractory ulcer, chronic inflammation after injury, infection and other types of wounds.

The present invention provides a medical composition which, in autotransplantation which is the only method that can induce the epithelialization even in a widespread full-thickness skin defect, enables the fixation of an autotransplantation skin graft in a simple manner and can increase the efficiency of epithelialization to promote the regeneration of the skin. The present invention elates to a medical composition comprising a photo-crosslinkable chitosan derivative and an amino acid and/or a saccharide. The amino acid is preferably an essential amino acid, and the saccharide is preferably a neutral saccharide selected from glucose, galactose, mannose and fucose.

The present invention is directed to methods that can be used in the treatment of wounds, burns, and photodamaged skin. Methods can be used for both humans and animals and involve the administration of compositions containing selegiline and/or desmethylselegiline.

The invention belongs to the field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composite for treating Qi-stagnation and blood-stasis type bedsores in clinical care. The composite is elaborately prepared from leaf of littelleaf paraquilegia, hairy clovershrub root, philippine violet herb, coral lodiscus flabellate, wild chrysanthemum, dysosma versipellis, radix angelicae, angelica sinensis and pearl powder which are proportioned by weight. The composite can invigorate the circulation of blood, remove stasis, regulate qi to alleviate pain, clear heat and promote dieresis, expel toxic substances, close sores, promote tissue regeneration, maintain vital qi, activate qi and blood, nourish viscera, and promote skin and hair growth so as to improve microcirculation of the area around the surface of a wound, increase local blood flow, relieve inflammatory edema, local tissue ischemia and anoxia and inflammatory exudation, and promote absorption and dissipation of an inflammation. Preferably, the dosage form is gel, so that the surface of the wound is kept wet, liquid medicine seepage is facilitated, newly formed granulation is not injured during dressing change, epithelization of the surface of the wound is facilitated, and healing of the surface of the wound is accelerated.