34results about How to "Promote epithelialization" patented technology

Transplantation of epithelial stem cells, cultured ex vivo on specifically treated amniotic membrane, yields, with that amniotic membrane, a surgical graft having expanded epithelial stem cells. The method of creating this graft and the graft itself provide simple and effective means to reconstruct damaged tissue, a preferred example being corneal tissue. The source of the epithelial stem cells can be a very small explant from healthy autologous and allogeneic tissue biopsy. The amniotic membrane is treated such that its extracellular matrix is maintained, but its cells are killed.

An occlusion device comprises a distal end, a proximal end and an elastic braided body which is provided between the proximal end and the distal end and made of wires. The elastic braided body is composed of a multi-stage braided net, which comprises at least a first-stage braided net that is closest to the distal end and made of a plurality of first-stage wires, and a second-stage braided net which is braided by a plurality of first-stage wires and second-stage wires all together. The minimum cross-section area of the first-stage braided net after being compressed toward the direction perpendicular to an axis of the elastic braided body is less than the minimum cross-section area of any other-stage braided net after being compressed toward the axis. A method for manufacturing the occlusion device is also provided.

The invention belongs to the technical field of natural high polymer materials, and particularly relates to a photoresponsive reversible chitosan hydrogel and a preparation method thereof. The preparation method comprises the following steps: by using biocompatible natural high polymer chitosan as an initial material, carrying out chemical modification to respectively introduce carboxymethyl group and coumarin group to the 6-O position and 2-N position, thereby obtaining water-soluble photoresponsive reversible chitosan; and carrying out specific-wavelength ultraviolet irradiation on the photoresponsive reversible chitosan water solution with certain concentration to obtain the chitosan hydrogel. The hydrogel can be converted into sol under certain-wavelength ultraviolet irradiation. The photoresponsive reversible chitosan gel-sol conversion can be implemented by pure controllable ultraviolet irradiation, thereby avoiding using the biologically toxic chemical reagents, enhancing the biocompatibility and use compliance of the chitosan hydrogel, and making the clinical application of the chitosan hydrogel possible. The invention provides a new way for clinical application of the chitosan hydrogel.

The invention discloses a method for preparing a photo-crosslinking chitosan hydrogel film. Biocompatible macromolecular chitosan as an initiator is subjected to chemical modification and introduced with hydroxypropyl and nitrine radicals at the 6-O site and N site respectively to form water-soluble photo-crosslinking chitosan; and the water solution of the photo-crosslinking chitosan is irradiated by ultraviolet light to form chitosan hydrogel so as to obtain the photo-crosslinking chitosan hydrogel film. The photo-crosslinking chitosan hydrogel film formed by a chitosan hydrogel film through ultraviolet light crosslinking by a physical method avoids chemical crosslinking with biological toxicity, improves the biocompatibility of the chitosan hydrogel film, realizes the clinical application of the chitosan hydrogel film, and provides a new path for the clinical application of the chitosan hydrogel film.

An occlusion device comprises a distal end, a proximal end and an elastic braided body which is provided between the proximal end and the distal end and made of wires. The elastic braided body is composed of a multi-stage braided net, which comprises at least a first-stage braided net that is closest to the distal end and made of a plurality of first-stage wires, and a second-stage braided net which is braided by a plurality of first-stage wires and second-stage wires all together. The minimum cross-section area of the first-stage braided net after being compressed toward the direction perpendicular to an axis of the elastic braided body is less than the minimum cross-section area of any other-stage braided net after being compressed toward the axis. A method for manufacturing the occlusion device is also provided.

The invention relates to a preparation method of a stem cell secreted factor flexible liposome. The preparation method comprises the steps that 0.100-0.150g of phospholipid, 0.020-0.030g of cholesterol, 0.2-0.3mg of vitamin E are dissolved by using 10ml of organic solvent, decompression and rotary evaporation are performed to remove the organic solvent and form a lipid film, a stem cell secreted factor solution is hydrated with the lipid film to form a stem cell secreted factor flexible liposome solution, the obtained stem cell secreted factor flexible liposome solution is subjected to homogenization treatment, and the stem cell secreted factor flexible liposome is obtained. The preparation method of the stem cell secreted factor flexible liposome effectively improves the retention time ofmesenchymal stem cell secreted factors, facilitates the adhesion and absorption of drugs and the skin, reduces stimulation and adverse effects, enhances the penetration ability to reach deep tissue,enhances the curative effects of secreted factors of mesenchymal stem cells, improves the utilization degree of the mesenchymal stem cells, and has no toxic side effects.

The invention relates to the technical field of tissue engineering and medical wound repairing, in particular to an amnion innate stem cell carried frozen active amnion particle and a conditioned medium and application thereof. Discarded fresh amnions are prepared into particles and then frozen into liquid nitrogen by means of serum-free stem cell freezing liquid; thus, complete matrix components of amnion are maintained while amnion epithelial cell activity is maintained for a long time, and further, disease transmission is avoided effectively. The conditioned medium which is prepared and collected by means of the frozen active amnion particles can promote chemotaxis and migration of human epidermal cells, fibroblast and endothelial cells effectively. In terms of zoografting, wound healing can be improved by multiple ways of regulating inflammatory reaction, promoting vascularization, quickening epithelization and the like through the active amnion particles; further, the amnion matrix can be directly used as a dermis equivalent to induce dermal regeneration so as to improve wound healing quality remarkably. The amnion innate stem cell carried frozen active amnion particle and the conditioned medium thereof can provide simple but effective ways for wound repairing.

The invention discloses a nigaichigosides F1 dressing for promoting scarless wound healing and a preparation method thereof, and belongs to the technical field of biomedical materials. It is proved that nigaichigosides F1 has the function of promoting wound healing and reducing scar formation. Meanwhile, the nigaichigosides F1 dressing for promoting wound healing and reducing scar formation is prepared by electrospinning. The nigaichigosides F1 dressing is a composite fiber membrane comprising the nigaichigosides F1 and zein, wheat gliadin, chitosan, hyaluronic acid or cellulose. he nigaichigosides F1 dressing is prepared by collection of affinity and moisturizing characteristics of a new wet dressing and the effect characteristics of promoting wound healing and reducing scar formation of the nigaichigosides F1, and has great medical application value for treatment of burns, incise injury, ulcers, pressure sores and other wounds.

The invention discloses a biological sheep membrane for ocular surface treatment and a preparation method thereof. The biological sheep membrane is prepared from sheep membrane through steps of rapid material obtaining, rapid material cutting, and radiation sterilization. The provided biological sheep membrane and preparation method have the following advantages: (1) various natural active components in sheep membrane can be effectively preserved; (2) the anti-inflammation and scar-resistant functions of natural active components in sheep membrane can be effectively preserved; (3) at the same time, the bacteria and viruses in the sheep membrane are completely killed, and thus the safety of the biological sheep membrane can be guaranteed; (4) the biological sheep membrane can be used to repair the damaged tissues, in the early period of treatment, the biological sheep membrane can promote the epithelization; and at the same time the biological sheep membrane can solve the problems that: (a) the material degrades too fast, and the curative effect cannot be achieved; (b) the material degrades too slowly, and the adverse irritation is generated on normal tissue.

The invention relates to a wound covering good in biocompatibility and capable of accelerating wound healing and a preparation method of the wound covering. The wound covering comprises a film sheet,and the film sheet is prepared from a carrier with good biocompatibility and high mechanical strength and protein containing dopa groups; the carrier is collagen, and the protein containing the dopa group is mussel mucin. The collagen is mixed with mussel mucin, a dry membrane is prepared by a semi-freeze-drying method under a low-temperature condition, the mussel mucin is immobilized on the collagen through chemical crosslinking, residual cross-linking agents are removed through rinsing, then the mussel mucin is stored in a preservation solution, the stability of the protein structure is enhanced, the activity of the collagen and the mussel mucin is maintained, and a collagen matrix has excellent mechanical strength and can be trimmed into any shape; tissue epithelialization can be accelerated, and wound healing is promoted; and the biocompatibility is good, the wound surface is not adhered, and secondary injury to the wound surface is avoided.

The invention provides a novel decellularized amnion preparation method, and relates to the technical field of biological materials. The method comprises ultrasonically treating the amnion and then physically scraping cells on the amnion to obtain the decellularized amnion. According to the method, the ultrasonic treatment and physical scraping mode is adopted to remove the cells on the surface ofthe decellularized amnion, the use of chemical reagents like trypsin is avoided, and the prepared decellularized amnion product is safer and reduces the residual chemical substances harmful to the body in the decellularized amnion. At the same time, the integrity of the amniotic matrix is retained to the utmost extent.

A hygienical comestic, especially a whitening cosmetic and its preparation method. It contains five materials of AíóBíóCíóD and E. A material comprises SS methyl gluoside, stearate, SSE-20 methyl gluoside, and addition compound of stearic acid and ethylene oxide, silicon oil DC-200 and son on. B material comprises CAD940,water, glycerol and so on. C material comprises TEA, water. D material comprises EGF, ATB-26000, STAY-C50. E material comprises essences.

The invention discloses a cracked nipple patch for women in a lactation period. The patch comprises a round hydrogel layer, a plastic protection film is arranged on the upper surface of the hydrogel layer, and a handle is arranged at the lower surface of the hydrogel layer. The hydrogel layer is made from the following components, by weight part, 15-20 parts of a seaweed active extract, 5-8 parts of collagen, 30-45 parts of an aloe extract, 3-5 parts of zinc chloride, 6-8 parts of vitamin B5, 3-5 parts of vitamin A, 1-3 parts of calcium lactate, 15-20 parts of agar and 15-20 parts of distilled water. The patch is excellent in one-way air permeability, can keep the area around a wound surface and a wound surface area in an environment with optimum humidity, is free of any toxic or side effect and can effectively prevent allergy. The patch is soft and free of adhesion, provides a long-lasting cool, refreshing and comfortable curative effect on the wound tissues, can alleviate pain, and can effectively promote the wound tissues to fast heal.

The invention belongs to the field of traditional Chinese medicine, and discloses a traditional Chinese medicine gel paste preparation for treating qi-blood deficiency decubitus in clinical nursing. The gel paste preparation is prepared from clear paste prepared from sida rhombifolia roots, alysicarpus vaginalis, lysimachia candida, beach morningglory herbs, rhizome polygoni runcinati, phaseolus lunatus, radix astragali, kadsura heteroclite stems and radix glycyrrhizae, carbomer, sodium polyacrylate, polyvinyl alcohol, sodium carboxymethylcellulose, propylene glycol, glycerinum, triethanolamine and water. The traditional Chinese medicine gel paste preparation has the effects of clearing heat, detoxicating, dragging toxicity, expelling pus, reducing swelling, easing pain, removing slough and promote tissue regeneration and is large in medicine containing quantity, high in moisture retention, good in compatibility with the skins and treatment effect and convenient to use.

The invention relates to application of an eIF3I-PDL1-IRS4 axis in preparation of a medicine for treating refractory ulcer. It is found that: when PDL1 is used for treating wounds, tissue formation, inflammation fading and reduction in vascularity can be induced; epithelization is accelerated; inflammation is relieved; healing of diabetic ulcer (DU) wounds is helped; in a DU wound surface, the expression of IRS4 is increased; the expression of the IRS4 of HaCaT cells is knocked down; the proliferation and migration of the cells are increased; when the eIF3I is over-expressed in HaCaT cells, the proliferation and migration of the cells are remarkably increased; and inflammatory infiltration is relieved. The invention prompts that the targeted eIF3I-PDL1-IRS4 axis can be used as a novel treatment method for treating refractory ulcer, chronic inflammation after injury, infection and other types of wounds.

The present invention provides a medical composition which, in autotransplantation which is the only method that can induce the epithelialization even in a widespread full-thickness skin defect, enables the fixation of an autotransplantation skin graft in a simple manner and can increase the efficiency of epithelialization to promote the regeneration of the skin. The present invention elates to a medical composition comprising a photo-crosslinkable chitosan derivative and an amino acid and / or a saccharide. The amino acid is preferably an essential amino acid, and the saccharide is preferably a neutral saccharide selected from glucose, galactose, mannose and fucose.

The invention discloses an antibacterial healing-promoting hydrogel dressing, which is prepared from 0.01 to 10 weight percent of antibacterial healing-promoting material, 1 to 20 weight percent of polyvinyl alcohol and 1 to 20 weight percent of gelatin. The dressing has a physical and chemical environment similar to that of a natural extracellular matrix, can stay at a wound part for a long time and exchange with body fluid, and provides nutrient substances and space for growth of cells and tissues; meanwhile, the antibacterial healing-promoting material in the dressing can effectively resist bacteria and control infection in time, and the healing-promoting effect can promote fibroblast proliferation so as to promote the epithelization process and promote wound healing. In addition, the invention also discloses a preparation method of the hydrogel dressing and application of the hydrogel dressing in preparation of medical wound dressings.

The present invention is directed to methods that can be used in the treatment of wounds, burns, and photodamaged skin. Methods can be used for both humans and animals and involve the administration of compositions containing selegiline and / or desmethylselegiline.

The invention relates to the technical field of cosmetics, in particular to a cosmetic or skin care preparation rich in growth factors and a preparation method thereof. The invention provides a growth factor-rich cosmetic or skin care product preparation composed of cell growth factor, small molecule peptide, water, oil, surfactant, moisturizer, antioxidant and preservative and its preparation method. The combination of the formulations of the composition can not only accelerate the metabolism of skin cells, repair the broken superficial dermal fibroblasts, have outstanding and rapid repair functions on skin wounds, but also have the functions of whitening and wrinkle removal, calming and anti-allergic, skin softening, Anti-wrinkle, whitening, refreshing, anti-aging, anti-radiation, prevention and lightening of spots and other functions.

The invention discloses an inorganic rapid hemostatic material and a preparation method thereof. The inorganic rapid hemostatic material is sepiolite fibers. The inorganic rapid hemostatic material has the following beneficial effects: 1, the inorganic rapid hemostatic material has the characteristics of quickly stopping bleeding of wounds, promoting wound healing, absorbing body fluid and maintaining the humidity of the wounds; 2, the liquid absorption capability is very strong, and the highest water absorption capability can reach 100%-120% of the mass of the material; 3, the hemostatic effect is good, the local concentration of platelets is increased, fibrin networking is promoted, and therefore the purpose of rapid hemostasis is achieved; 4, the humidity of a wound surface can be adjusted, the hemostatic material is high in water retention capacity, can be slowly released after absorbing wound secretion and moisture, can keep the humidity of the wound surface and is beneficial to cell proliferation and epithelization, so that wound healing is promoted, and silver nano particles attached to sepiolite fibers have sterilization and anti-infection effects; 5, the porosity is high,oxygen can pass through, bacteria are isolated, and tissue growth is facilitated; 6, the hemostatic material does not contain any organic solvent, binder or other toxic and harmful substances, and will not pollute the environment; and 7, the hemostatic material has good processability and hydrophilicity, so that the hemostatic material can be easily mixed with chitosan, alginate, and the like, andused as a wound auxiliary material.

The invention discloses a method for preparing an artificial esophagus with a biological structure and biological functions. The method is characterized by comprising the steps: taking a fresh mammalesophagus, leaving a mucous membrane and a submucous layer, then, carrying out decellularization treatment, and carrying out freeze-drying preservation; then, taking a fresh placenta amniotic membrane, leaving complete amniotic membrane tissue containing an epithelial cell layer, a basement membrane and a blood-vessel-free matrix, then, preparing a decellularized amniotic membrane, then, tiling the decellularized amniotic membrane on a freeze-dried esophagus mucous membrane, and carrying out air-drying at room temperature, thereby obtaining the artificial esophagus with the biological structure and the biological functions. The method has the advantages that the artificial esophagus prepared by the method simulates normal esophagus tissue of a human body in a macroscopic form and a microstructure; the artificial esophagus can successfully achieve epithelization, and thus, the biological functions of the esophagus tissue can be exerted to the maximum functionally, so that the artificialesophagus can be more suitable for replacing part of a pathological-changed esophagus of a patient or sick animal.

The invention relates to a wound covering which has good biocompatibility and can accelerate wound healing and a preparation method thereof. The wound covering comprises a thin film; the thin film is made of a carrier with good biocompatibility and high mechanical strength. It is prepared with a protein containing a dopa group; the carrier is collagen; and the protein containing a dopa group is mussel mucin. In the present invention, collagen and mussel mucin are mixed, and a dry membrane is made by semi-lyophilization under low temperature conditions, and then the mussel mucin is fixed on the collagen by chemical crosslinking, and the residual crosslinking agent is removed by rinsing. After being stored in the preservation solution, the stability of the protein structure is enhanced and the activity of collagen and mussel mucin is maintained. The collagen matrix has excellent mechanical strength and can be trimmed into any shape; it can accelerate tissue epithelialization and promote wound healing. Healing; good biocompatibility; no adhesion to the wound surface, no secondary damage to the wound surface.

The invention discloses an inorganic rapid hemostatic material and a preparation method thereof. The inorganic rapid hemostatic material is sepiolite fiber. The present invention has the following beneficial effects: 1. It has the characteristics of quick hemostasis of wound, promotion of wound healing, absorption of body fluid and maintenance of wound humidity; 2. Very strong liquid absorption capacity, the highest water absorption capacity can reach 100%-120% of its own mass; 3. The hemostatic effect is good, which increases the local concentration of platelets and promotes the network of fibrin, so as to achieve the purpose of rapid hemostasis; 4. It can adjust the humidity of the wound surface. release, maintain the humidity of the wound surface, is conducive to cell proliferation and epithelialization, thereby promoting wound healing, and attaching silver nanoparticles to sepiolite fibers has bactericidal and anti-infective effects; 5. High porosity allows oxygen to pass through and isolates bacteria , helps tissue growth; 6. Does not contain organic solvents, binders, and other toxic and harmful substances, and will not pollute the environment after use; 7. Good processing performance and hydrophilicity make this hemostatic material easy to combine with chitosan , alginate, etc. are used as wound materials.

The invention belongs to the field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composite for treating Qi-stagnation and blood-stasis type bedsores in clinical care. The composite is elaborately prepared from leaf of littelleaf paraquilegia, hairy clovershrub root, philippine violet herb, coral lodiscus flabellate, wild chrysanthemum, dysosma versipellis, radix angelicae, angelica sinensis and pearl powder which are proportioned by weight. The composite can invigorate the circulation of blood, remove stasis, regulate qi to alleviate pain, clear heat and promote dieresis, expel toxic substances, close sores, promote tissue regeneration, maintain vital qi, activate qi and blood, nourish viscera, and promote skin and hair growth so as to improve microcirculation of the area around the surface of a wound, increase local blood flow, relieve inflammatory edema, local tissue ischemia and anoxia and inflammatory exudation, and promote absorption and dissipation of an inflammation. Preferably, the dosage form is gel, so that the surface of the wound is kept wet, liquid medicine seepage is facilitated, newly formed granulation is not injured during dressing change, epithelization of the surface of the wound is facilitated, and healing of the surface of the wound is accelerated.

The invention discloses a H2S donor compound capable of being used for skin dressing, sponge dressing and a preparation method, and relates to H2S donor compounds, sponge dressing and preparation methods. The invention firstly aims to provide a H2S donor compound CW-1 capable of being used for skin dressing. The compound can transfer H2S gas molecules to skin specifically and byproducts thereof have the antibacterial action. The invention secondarily aims to provide dressing suitable for skin. The dressing is sponge dressing of calcium alginate comprising the H2S donor compound CW-1. The dressing can be in contact with skin wounds, can transfer H2S gas molecules of a therapeutic dose stably to the skin of wounds effectively, and plays an antibacterial role.

[Object]The present invention is intended to provide a urethral stricture treatment agent and a urethral stricture treatment method capable of avoiding restenosis by a less invasive transurethral endoscopic procedure in urethral stricture treatment.[Solution]A urethral stricture treatment agent including at least a hydrogel-forming polymer, and having a storage elastic modulus of 50 Pa or less at 10° C. and a storage elastic modulus of 100 Pa or more at 37° C.A urethral stricture treatment method at least including injecting the urethral stricture treatment agent cooled to 10° C. or lower into an inner surface of urethra which has been incised with a transurethral endoscopic procedure, and holding the urethral stricture treatment agent in the inner surface of urethra at a temperature not lower than a room temperature.

The present invention is Chinese medicine rinsing liquid for use after nasal endoscope operation and belongs to the field of Chinese medicine preparation. The Chinese medicine rinsing liquid is prepared with 12 kinds of Chinese medicinal materials, including magnolia flower, cocklebur fruit, dahurian angelica root, asarum herb, Chuanxiong rhizome, etc in certain weight proportion. It has the functions of dispelling wind and heat, promoting blood circulation, relieving swelling, promoting the dispersing function of lung and relieving stasis, and has obvious curative effect, no irritation and no toxic side effect.

The invention relates to the technical field of tissue engineering and medical wound repairing, in particular to an amnion innate stem cell carried frozen active amnion particle and a conditioned medium and application thereof. Discarded fresh amnions are prepared into particles and then frozen into liquid nitrogen by means of serum-free stem cell freezing liquid; thus, complete matrix components of amnion are maintained while amnion epithelial cell activity is maintained for a long time, and further, disease transmission is avoided effectively. The conditioned medium which is prepared and collected by means of the frozen active amnion particles can promote chemotaxis and migration of human epidermal cells, fibroblast and endothelial cells effectively. In terms of zoografting, wound healing can be improved by multiple ways of regulating inflammatory reaction, promoting vascularization, quickening epithelization and the like through the active amnion particles; further, the amnion matrix can be directly used as a dermis equivalent to induce dermal regeneration so as to improve wound healing quality remarkably. The amnion innate stem cell carried frozen active amnion particle and the conditioned medium thereof can provide simple but effective ways for wound repairing.

The invention discloses a biological sheep membrane for ocular surface treatment and a preparation method thereof. The biological sheep membrane is prepared from sheep membrane through steps of rapid material obtaining, rapid material cutting, and radiation sterilization. The provided biological sheep membrane and preparation method have the following advantages: (1) various natural active components in sheep membrane can be effectively preserved; (2) the anti-inflammation and scar-resistant functions of natural active components in sheep membrane can be effectively preserved; (3) at the same time, the bacteria and viruses in the sheep membrane are completely killed, and thus the safety of the biological sheep membrane can be guaranteed; (4) the biological sheep membrane can be used to repair the damaged tissues, in the early period of treatment, the biological sheep membrane can promote the epithelization; and at the same time the biological sheep membrane can solve the problems that: (a) the material degrades too fast, and the curative effect cannot be achieved; (b) the material degrades too slowly, and the adverse irritation is generated on normal tissue.