Antibacterial healing-promoting hydrogel dressing as well as preparation method and application thereof

A technology of healing hydrogel and healing materials, which is applied in the field of antibacterial and healing-promoting hydrogel dressings and its preparation, can solve the problems of unsatisfactory biocompatibility of silver-containing dressings, high toxicity of silver ions, and toxic and side effects, so as to promote Effects on fibroblast proliferation, infection control, broad application potential

Active Publication Date: 2022-04-08
TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at the same time, silver may enter the human body through wounds and accumulate, leading to cell death and toxic side effects (Min Ji Hong et al., Polymers, 2018, 10(10). Shahin Homaeigohar and AldoR. Boccaccini. Acta Biomaterialia, 2020 ,107:25-49.) Therefore, the biocompatibility of silver-containing dressings is not ideal
For example, although silver sulfadiazine cream is often used clinically for the treatment of infected wounds, due to the high toxicity of silver ions to normal tissue cells, especially the obvious hindrance to the epithelialization process, it is clearly pointed out in its instructions that once The drug should be discontinued immediately when epithelialization begins

Method used

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  • Antibacterial healing-promoting hydrogel dressing as well as preparation method and application thereof
  • Antibacterial healing-promoting hydrogel dressing as well as preparation method and application thereof
  • Antibacterial healing-promoting hydrogel dressing as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] S1: Weigh 0.16g with a molecular weight of 5×10 4 Da, chitosan with a deacetylation degree of 98% was added to 10mL MES buffer (25mM, pH=4.80), and 0.1mL HCl was added dropwise and stirred at room temperature for half an hour to completely dissolve the chitosan, thereby obtaining the mass volume percentage Concentration is the homogeneous solution of 1.6%; Then the mixed solution (solvent is the MES buffering of 25mM pH=4.80 that the molecular weight of activation at room temperature is 1000Da carboxyl-polyethylene glycol-azide group, NHS and EDC·HCl solution) 20mL was added in the above-mentioned reaction solution, and the stirring reaction was continued at room temperature for 24 hours, wherein the ratio of the amount of substances of chitosan, carboxyl-polyethylene glycol-azido, NHS, EDC·HCl was 1:2: 4:4; After the reaction is finished, add hydroxylamine hydrochloride in the amount of carboxyl-polyethylene glycol-azido and other substances to terminate the reaction, ...

Embodiment 2

[0051] S1: Weigh an appropriate amount of antibacterial polypeptide WR into a centrifuge tube, add an appropriate amount of sterile saline to dissolve, and obtain a sample solution of 256 μg / mL.

[0052] S2: Weigh an appropriate amount of polyvinyl alcohol (PVA) with a degree of alcoholysis of 99% in a round bottom flask, and add an appropriate amount of physiological saline therein so that the mass fraction of PVA is 10%. Add a magneton into the round bottom flask, heat and stir at 90°C, 550rpm for 12h, until the PVA is fully dissolved. Transfer the PVA solution to a glass sample bottle and transfer it to a sterilizer for sterilization at 121°C for 20 minutes for later use.

[0053] S3: Weigh an appropriate amount of type A gelatin in a centrifuge tube, and add an appropriate amount of sterile saline thereinto make the gelatin mass fraction 5%. Place the centrifuge tube in a shaker at 37°C for 4 hours to obtain a uniform gelatin solution for later use.

[0054] S4: Mix WR, ...

Embodiment 3

[0056] S1: A certain amount of chitosan was dissolved in 2-(N-morpholine)ethanesulfonic acid (MES) buffer to form solution 1, and polyethylene glycol-azide with carboxyl groups was added to the buffer Form solution 2, add 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC hydrochloride) and N-hydroxysuccinimide to activate carboxyl group in solution 2, room temperature Or mix solution 1 and solution 2 after shaking at 4°C for 5-120 minutes, shake at room temperature or at 4°C for 6-72 hours, dialyze the sample and freeze-dry to obtain chitosan modified with azide-polyethylene glycol.

[0057] Dissolve the azide-polyethylene glycol-modified chitosan and the alkyne-modified antibacterial polypeptide WR in 2-(N-morpholine)ethanesulfonic acid buffer, add the catalyst solution, shake at room temperature or 4 degrees React for 10 minutes to 48 hours. After the reaction is completed, add excess ethylenediaminetetraacetic acid, vibrate, dialyze, and freeze-dry to obtain ...

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Abstract

The invention discloses an antibacterial healing-promoting hydrogel dressing, which is prepared from 0.01 to 10 weight percent of antibacterial healing-promoting material, 1 to 20 weight percent of polyvinyl alcohol and 1 to 20 weight percent of gelatin. The dressing has a physical and chemical environment similar to that of a natural extracellular matrix, can stay at a wound part for a long time and exchange with body fluid, and provides nutrient substances and space for growth of cells and tissues; meanwhile, the antibacterial healing-promoting material in the dressing can effectively resist bacteria and control infection in time, and the healing-promoting effect can promote fibroblast proliferation so as to promote the epithelization process and promote wound healing. In addition, the invention also discloses a preparation method of the hydrogel dressing and application of the hydrogel dressing in preparation of medical wound dressings.

Description

technical field [0001] The invention relates to the technical field of medical materials. More specifically, it relates to an antibacterial and healing-promoting hydrogel dressing and its preparation method and application. Background technique [0002] Skin trauma, including burns, surgical trauma, and venous leg ulcers, imposes a serious burden on society and the medical system. When the wound is infected by pathogenic microorganisms such as Staphylococcus aureus and Pseudomonas aeruginosa, it will further prolong the healing time of the wound, increase the cost of treatment, and in severe cases, cause systemic sepsis, eventually leading to tissue necrosis or even amputation . Therefore, the treatment and repair of infected wounds has become a major national demand. [0003] The classic wound healing process mainly includes three stages. In the inflammatory stage, the blood clot formed by hemostasis becomes the scaffold of immune cells, and at the same time immune cell...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L26/00
CPCY02A50/30
Inventor 朱萌牛忠伟陈禹州鞠晓燕
Owner TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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