Biological sheep membrane for ocular surface treatment and preparation method thereof

A kind of amniotic membrane and biological technology, applied in the field of medical materials, can solve the problems of becoming brittle and easy to tear, and achieve the effect of inhibiting scar formation, low processing intensity, and good clinical application prospects

Active Publication Date: 2015-08-12
广州瑞泰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The prepared amnion, similar to the above-mentioned patented method, has undergone freeze-drying, which has caused damage to the amnion to a certain extent, making it brittle and easy to tear in clinical use;

Method used

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  • Biological sheep membrane for ocular surface treatment and preparation method thereof
  • Biological sheep membrane for ocular surface treatment and preparation method thereof
  • Biological sheep membrane for ocular surface treatment and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The preparation method of the biological amniotic membrane that is used for ocular surface treatment, its steps are as follows:

[0041] (1)Quick collection of materials

[0042] Use the amniotic membrane sampling device to quickly obtain the amniotic membrane outside the placenta within 1 minute, quickly put the fresh amniotic membrane into a transport bottle pre-filled with sterile saline, complete cooling to 10°C within 1 minute after the amniotic membrane is separated from the mother, and refrigerate. Transport to the clean workshop within 1 hour for subsequent processing.

[0043] (2) Quick cutting

[0044] The amniotic membrane obtained in step (1) is spread on the nitrocellulose filter membrane with the epithelial side facing up, and the amniotic membrane is cut into a square amniotic membrane piece with a length of 70mm and a width of 70mm within 1 minute with the amniotic membrane cutting device, and then cut to obtain The amniotic membrane sheet is packed in...

Embodiment 2

[0049] The preparation method of the biological amniotic membrane that is used for ocular surface treatment, its steps are as follows:

[0050] (1)Quick collection of materials

[0051] Use the amniotic membrane sampling device to quickly obtain the amniotic membrane outside the placenta within 3 minutes, quickly put the fresh amniotic membrane into a transport bottle pre-filled with sterile saline, and complete the cooling to 2°C within 10 minutes after the amniotic membrane is separated from the mother. Transport to the clean workshop within 1 hour for subsequent processing.

[0052] (2) Quick cutting

[0053] The amniotic membrane obtained in step (1) is spread on the nitrocellulose filter membrane with the epithelial side facing up, and the amniotic membrane is cut into a rectangular amniotic membrane with a length of 10mm and a width of 15mm within 3 minutes by using the punching amniotic membrane cutting device invented by our company Membranes, and then the amniotic m...

Embodiment 3

[0058] The preparation method of the biological amniotic membrane that is used for ocular surface treatment, its steps are as follows:

[0059] (1)Quick collection of materials

[0060] The amniotic membrane outside the placenta is quickly obtained within 2 minutes using the amniotic membrane sampling device, and the fresh amniotic membrane is quickly put into a transport bottle pre-filled with sterile saline, and the amniotic membrane is cooled to 6°C within 5 minutes of detaching from the mother's body, and refrigerated. Transport to the clean workshop within 1 hour for subsequent processing.

[0061] (2) Quick cutting

[0062] The amniotic membrane obtained in step (1) is spread on the nitrocellulose filter membrane with the epithelial side facing up, and the amniotic membrane cutting device invented by our company is used to cut the amniotic membrane into circular amniotic membrane pieces with a diameter of 50 mm within 2 minutes. Then the cut amniotic membrane sheet is ...

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Abstract

The invention discloses a biological sheep membrane for ocular surface treatment and a preparation method thereof. The biological sheep membrane is prepared from sheep membrane through steps of rapid material obtaining, rapid material cutting, and radiation sterilization. The provided biological sheep membrane and preparation method have the following advantages: (1) various natural active components in sheep membrane can be effectively preserved; (2) the anti-inflammation and scar-resistant functions of natural active components in sheep membrane can be effectively preserved; (3) at the same time, the bacteria and viruses in the sheep membrane are completely killed, and thus the safety of the biological sheep membrane can be guaranteed; (4) the biological sheep membrane can be used to repair the damaged tissues, in the early period of treatment, the biological sheep membrane can promote the epithelization; and at the same time the biological sheep membrane can solve the problems that: (a) the material degrades too fast, and the curative effect cannot be achieved; (b) the material degrades too slowly, and the adverse irritation is generated on normal tissue.

Description

【Technical field】 [0001] The invention relates to the field of medical materials, in particular to a biological amniotic membrane used for ocular surface treatment and a preparation method thereof. 【Background technique】 [0002] The amniotic membrane, derived from the cytotrophoblast, is the inner layer of the two-layered human fetal membranes and consists of an epithelial cell layer, a basement membrane, and an avascular stroma. Fresh amnion contains a variety of collagens, laminin, fibronectin, glycosaminoglycans, hyaluronic acid, etc. These active substances can promote the adhesion and migration of epithelial cells, induce epithelial differentiation, and prevent epithelial apoptosis; amnion cells and The matrix also contains a variety of growth factors, such as nerve growth factor (NGF), hepatocyte growth factor (HGF), keratinocyte growth factor (KGF), transforming growth factor-α (TGF-α), transforming growth factor-β1 ( TGF-β1), epidermal growth factor (EGF), basic fi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/50A61P17/02A61P29/00A61P9/00
Inventor 刘博文王二浩刘艳丽何海娜叶伟初罗锦荣王恩光林永亮谢丽君
Owner 广州瑞泰生物科技有限公司
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