A kind of biological amniotic membrane used for ocular surface treatment and preparation method thereof
An amniotic membrane and biological technology, applied in the field of medical materials, can solve the problems of brittleness and easy tearing, and achieve the effects of inhibiting scar formation, low processing intensity, and good clinical application prospects.
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Embodiment 1
[0040] The preparation method of the biological amniotic membrane that is used for ocular surface treatment, its steps are as follows:
[0041] (1)Quick collection of materials
[0042] Use the amniotic membrane sampling device to quickly obtain the amniotic membrane outside the placenta within 1 minute, quickly put the fresh amniotic membrane into a transport bottle pre-filled with sterile saline, complete cooling to 10°C within 1 minute after the amniotic membrane is separated from the mother, and refrigerate. Transport to the clean workshop within 1 hour for subsequent processing.
[0043] (2) Quick cutting
[0044] The amniotic membrane obtained in step (1) is spread on the nitrocellulose filter membrane with the epithelial side facing up, and the amniotic membrane is cut into a square amniotic membrane piece with a length of 70mm and a width of 70mm within 1 minute with the amniotic membrane cutting device, and then cut to obtain The amniotic membrane sheet is packed in...
Embodiment 2
[0049] The preparation method of the biological amniotic membrane that is used for ocular surface treatment, its steps are as follows:
[0050] (1)Quick collection of materials
[0051] Use the amniotic membrane sampling device to quickly obtain the amniotic membrane outside the placenta within 3 minutes, quickly put the fresh amniotic membrane into a transport bottle pre-filled with sterile saline, and complete the cooling to 2°C within 10 minutes after the amniotic membrane is separated from the mother. Transport to the clean workshop within 1 hour for subsequent processing.
[0052] (2) Quick cutting
[0053] The amniotic membrane obtained in step (1) is spread on the nitrocellulose filter membrane with the epithelial side facing up, and the amniotic membrane is cut into a rectangular amniotic membrane with a length of 10mm and a width of 15mm within 3 minutes by using the punching amniotic membrane cutting device invented by our company Membranes, and then the amniotic m...
Embodiment 3
[0058] The preparation method of the biological amniotic membrane that is used for ocular surface treatment, its steps are as follows:
[0059] (1)Quick collection of materials
[0060] The amniotic membrane outside the placenta is quickly obtained within 2 minutes using the amniotic membrane sampling device, and the fresh amniotic membrane is quickly put into a transport bottle pre-filled with sterile saline, and the amniotic membrane is cooled to 6°C within 5 minutes of detaching from the mother's body, and refrigerated. Transport to the clean workshop within 1 hour for subsequent processing.
[0061] (2) Quick cutting
[0062] The amniotic membrane obtained in step (1) is spread on the nitrocellulose filter membrane with the epithelial side facing up, and the amniotic membrane cutting device invented by our company is used to cut the amniotic membrane into circular amniotic membrane pieces with a diameter of 50 mm within 2 minutes. Then the cut amniotic membrane sheet is ...
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