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Method for preparing artificial esophagus with biological structure and biological functions

An artificial esophagus, biological technology, applied in the field of biomedical engineering, to achieve the effect of promoting epithelialization

Pending Publication Date: 2018-01-16
杭州恩格生物医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But this is only a general method for preparing scaffolds suitable for various organs, and it does not make the scaffolds have the unique biological functions of esophagus according to the actual microstructure of human esophagus.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment one: a kind of preparation method of artificial esophagus with biological structure and function, comprises the following specific steps:

[0026] (1) Clean the fresh pig esophagus, peel off the muscle layer, and leave the mucosa and submucosa;

[0027] (2) Rinse the mucosa and submucosa in alcohol with a volume concentration of 75% for 10 minutes, and then wash away the alcohol with water;

[0028] (3) Immerse the mucosa and submucosa in Triton 100 with a mass concentration of 5% for 1 day, change the solution every 12 hours during this period, and finally wash with PBS;

[0029] (4) Immerse the mucosa and submucosa in the pancrelipase / PBS solution at 30°C for 20 hours, wherein: the concentration of pancrelipase in the solution is 1000U / L, and then take out the alcohol with a volume concentration of 75%. Rinse in medium, then rinse with PBS;

[0030] (5) Immerse the mucosa and submucosa in DNase / PBS solution at 40°C for 2 hours, wherein: the concentration ...

Embodiment 2

[0038] Embodiment two: a kind of preparation method of artificial esophagus with biological structure and function, comprises the following specific steps:

[0039] (1) Clean the fresh rabbit esophagus, peel off the muscle layer, and leave the mucosa and submucosa;

[0040] (2) Rinse the mucosa and submucosa in alcohol with a volume concentration of 85% for 7 minutes, and then wash off the alcohol with water;

[0041] (3) Immerse the mucosa and submucosa in 1% Triton 100 for 5 days, during which the solution was changed every 12 hours, and finally washed with PBS;

[0042] (4) Immerse the mucosa and submucosa in the trylipase / PBS solution at 40°C for 7 hours, wherein the concentration of trypsin in the solution is 3000U / L, and then take out the alcohol with a volume concentration of 75%. Rinse in medium, then rinse with PBS;

[0043] (5) Immerse the mucosa and submucosa in a DNase / PBS solution at a temperature of 35°C for 8 hours, wherein: the concentration of DNase in the s...

Embodiment 3

[0051] Embodiment three: a kind of preparation method of artificial esophagus with biological structure and function, comprises the following concrete steps:

[0052] (1) Clean the fresh sheep esophagus, peel off the muscle layer, and leave the mucosa and submucosa;

[0053] (2) Rinse the mucosa and submucosa in alcohol with a volume concentration of 90% for 5 minutes, and then wash off the alcohol with water;

[0054] (3) Immerse the mucosa and submucosa in Triton 100 with a mass concentration of 3% for 3 days, during which the solution was changed every 12 hours, and finally washed with PBS;

[0055] (4) Immerse the mucosa and submucosa in the trylipase / PBS solution at a temperature of 35°C for 12 hours, wherein: the concentration of trypsin in the solution is 5000U / L, and then take out the alcohol with a volume concentration of 85%. Rinse in medium, then rinse with PBS;

[0056] (5) Immerse the mucosa and submucosa in a DNase / PBS solution at a temperature of 30°C for 10 h...

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Abstract

The invention discloses a method for preparing an artificial esophagus with a biological structure and biological functions. The method is characterized by comprising the steps: taking a fresh mammalesophagus, leaving a mucous membrane and a submucous layer, then, carrying out decellularization treatment, and carrying out freeze-drying preservation; then, taking a fresh placenta amniotic membrane, leaving complete amniotic membrane tissue containing an epithelial cell layer, a basement membrane and a blood-vessel-free matrix, then, preparing a decellularized amniotic membrane, then, tiling the decellularized amniotic membrane on a freeze-dried esophagus mucous membrane, and carrying out air-drying at room temperature, thereby obtaining the artificial esophagus with the biological structure and the biological functions. The method has the advantages that the artificial esophagus prepared by the method simulates normal esophagus tissue of a human body in a macroscopic form and a microstructure; the artificial esophagus can successfully achieve epithelization, and thus, the biological functions of the esophagus tissue can be exerted to the maximum functionally, so that the artificialesophagus can be more suitable for replacing part of a pathological-changed esophagus of a patient or sick animal.

Description

technical field [0001] The invention belongs to the field of biomedical engineering, in particular to a method for preparing an artificial esophagus with biological structure and function. Background technique [0002] The normal human esophagus is a hollow tube about 25 centimeters long, connecting the throat and stomach, and mainly plays the role of transporting food and water. The esophagus is composed of mucosa, submucosa, muscle layer and adventitia. The mucosa is composed of non-keratinized stratified squamous epithelial cells, which grow on the basement membrane, which provides the special proteins, growth factors and other nutrients required for cell growth. Substances to regulate cell proliferation, differentiation, migration and death. The muscular layer is composed of muscle cells, mainly smooth muscle cells. The mucosa and submucosa together play the role of secreting mucus and preventing food and water leakage. It is the most important part for the normal funct...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/36A61L27/50
Inventor 竺亚斌侯雷王健高瑞何灿洋
Owner 杭州恩格生物医疗科技有限公司
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