Method for preparing photo-crosslinking chitosan hydrogel film

A technology of crosslinking chitosan and photocrosslinking, which is applied in medical science, absorbent pads, bandages, etc., can solve problems such as cytotoxicity, and achieve the effects of promoting epithelialization, high environmental friendliness, and good biodegradability

Inactive Publication Date: 2009-09-16
JIANGNAN UNIV
View PDF3 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the research results of Draye et al., trace amounts of dialdehydes may be released from the hydrogel and cause the cytotoxicity of the hydrogel

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing photo-crosslinking chitosan hydrogel film

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: the preparation of water-soluble hydroxypropyl chitosan

[0020] 40g of chitosan was added into 250ml of isopropanol and stirred for 30 minutes. Then 30 ml of sodium hydroxide solution (33%, w / v) was added to the mixture. The mixed solution was stirred for 1 h and added to the flask at room temperature, sealed, and frozen overnight at -20 °C. 40 ml of 10% (v / v) catalyst solution (tetramethylammonium hydroxide) was added dropwise to the mixture, followed by 400 ml of propylene oxide. The reaction was stirred at room temperature for 1 h, and the reaction temperature was slowly raised to 60° C., and the reaction time was 7 h. Add the reaction solution to 4 times the volume of ethanol solution to obtain a precipitate, wash the precipitate twice with 10 times the volume of ethanol, redissolve the precipitate with deionized water, dialyze and freeze-dry to obtain high-purity hydroxypropyl chitosan .

Embodiment 2

[0021] Embodiment 2: Preparation of photo-crosslinkable chitosan

[0022] Get 10g of prepared hydroxypropyl chitosan and dissolve it in 50ml of 50mM TEMED solution and stir until the hydroxypropyl chitosan is completely dissolved, then add 5g of EDC and 10g of 4-azidobenzoic acid. The reaction was stirred for 50 h at room temperature at 25°C. Dialyzed in deionized water for two days and freeze-dried to obtain photocrosslinkable chitosan.

Embodiment 3

[0023] Embodiment 3: Preparation of photocrosslinked chitosan hydrogel film

[0024] 400 μl of photo-crosslinkable chitosan aqueous solution with a concentration of 40 mg / ml was spread in a petri dish with a diameter of 7 cm. A petri dish coated with photo-crosslinkable chitosan was irradiated with ultraviolet light with a wavelength of 365 nm and a power of 20 W for 500 s to obtain a photo-crosslinkable chitosan hydrogel film with a film thickness of about 0.1 mm.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
Login to view more

Abstract

The invention discloses a method for preparing a photo-crosslinking chitosan hydrogel film. Biocompatible macromolecular chitosan as an initiator is subjected to chemical modification and introduced with hydroxypropyl and nitrine radicals at the 6-O site and N site respectively to form water-soluble photo-crosslinking chitosan; and the water solution of the photo-crosslinking chitosan is irradiated by ultraviolet light to form chitosan hydrogel so as to obtain the photo-crosslinking chitosan hydrogel film. The photo-crosslinking chitosan hydrogel film formed by a chitosan hydrogel film through ultraviolet light crosslinking by a physical method avoids chemical crosslinking with biological toxicity, improves the biocompatibility of the chitosan hydrogel film, realizes the clinical application of the chitosan hydrogel film, and provides a new path for the clinical application of the chitosan hydrogel film.

Description

technical field [0001] The invention belongs to the technical field of natural polymer materials, and in particular relates to a preparation method of a photocrosslinked chitosan hydrogel film. Background technique [0002] The development of novel wound-healing membranes has attracted increasing attention over the past decades. It is generally believed that an ideal wound healing film should not only protect the wound from bacterial infection, but also form a biocompatible and moist healing environment. Traditional (non-airtight) wound healing membranes can only provide basic healing conditions for wounds, but they are still the leading products in the current market. Wound healing hydrogel membrane is an airtight membrane that can provide a moist healing environment for wounds, and its application prospects are extremely wide. [0003] The hydrogel formed by hydrophilic polymers through various cross-linking effects has the lowest inflammatory response, thrombus and tiss...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/28A61L15/60C08J5/18C08J3/075C08L5/08C08B37/08
Inventor 陈敬华金坚许正宏凌凯
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap