Application of eIF3I-PDL1-IRS4 axis in preparation of medicine for treating refractory ulcer

A refractory ulcer, 1. PDL1 technology, applied in the field of biomedicine, to achieve the effect of overcoming proliferation and migration, good epithelialization, and reducing inflammation

Active Publication Date: 2021-09-17
SHANGHAI DERMATOLOGY HOSPITAL
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the eIF3I-PDL1-IRS4 axis in the treatment of refractory ulcers

Method used

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  • Application of eIF3I-PDL1-IRS4 axis in preparation of medicine for treating refractory ulcer
  • Application of eIF3I-PDL1-IRS4 axis in preparation of medicine for treating refractory ulcer
  • Application of eIF3I-PDL1-IRS4 axis in preparation of medicine for treating refractory ulcer

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Experimental program
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Embodiment 1

[0063] 1. Methods and materials

[0064] 1. Clinical samples

[0065] Clinical samples were provided by patients with written informed consent, and were approved by the Ethics Committee of Yueyang Hospital ( Figure 25 ). All patients underwent surgical treatment in surgery from April 2019 to February 2020. DU samples were collected from the margins of unhealed wounds during surgical debridement, and normal skin samples were collected from excess skin collected during cosmetic surgery. Immediately after the tissue was cut, it was fixed with 70% formalin and embedded in paraffin. The clinical characteristics of the patients are shown in Table 1.

[0066] Table 1 Clinical patient characteristics

[0067]

[0068] 2. Cell culture and transfection

[0069] The human keratinocyte cell line HaCaT was purchased from Cell Lines Service, Eppelheim, Germany. Cells were cultured in DMEM-high glucose (4.5 g / L, Gibco, Life Technologies, Carlsbad, CA) containing 10% (v / v) fetal bo...

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Abstract

The invention relates to application of an eIF3I-PDL1-IRS4 axis in preparation of a medicine for treating refractory ulcer. It is found that: when PDL1 is used for treating wounds, tissue formation, inflammation fading and reduction in vascularity can be induced; epithelization is accelerated; inflammation is relieved; healing of diabetic ulcer (DU) wounds is helped; in a DU wound surface, the expression of IRS4 is increased; the expression of the IRS4 of HaCaT cells is knocked down; the proliferation and migration of the cells are increased; when the eIF3I is over-expressed in HaCaT cells, the proliferation and migration of the cells are remarkably increased; and inflammatory infiltration is relieved. The invention prompts that the targeted eIF3I-PDL1-IRS4 axis can be used as a novel treatment method for treating refractory ulcer, chronic inflammation after injury, infection and other types of wounds.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of the eIF3I-PDL1-IRS4 axis in the preparation of refractory ulcer medicine. Background technique [0002] The skin is the organ that the human body has the most contact with the environment, and is directly exposed to the surrounding environment. It has functions such as sensory and immune protection, and is also vulnerable to injury and infection. Wound healing is impaired in diabetes, vascular disease, and elderly patients. As the diabetic population grows, the impact of diabetic ulcers (DUs) on healthcare costs is becoming increasingly significant. Diabetic ulcer is a complication of diabetes, which occurs mostly on the feet of patients, so it is also called diabetic foot. Patients with long-term diabetes often have vascular lesions and complications of peripheral neuritis. The feeling of the toes of such patients is often lost due to neuritis, so they ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17A61K45/00A61P3/10A61P17/02A61P29/00
CPCA61K38/1774A61K38/1709A61K45/00A61P17/02A61P3/10A61P29/00
Inventor 李斌蒯仂茹意向延卫陈启龙罗楹宋建坤朱全刚朱聪聪江静斯李欣张颖张展周蜜
Owner SHANGHAI DERMATOLOGY HOSPITAL
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