Preparation method of core-shell nanoparticles based on lipid membrane and metal organic framework

A technology of metal-organic frameworks and nanoparticles, which is applied to medical preparations with non-active ingredients, medical preparations containing active ingredients, organic active ingredients, etc., can solve problems such as normal cell and tissue damage, and avoid random release, Uniform and stable particles, strong modifiable effect

Active Publication Date: 2020-06-12
SHANGHAI NAT ENG RES CENT FORNANOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, after drug carriers carry chemotherapeutic drugs into the body, their release may

Method used

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  • Preparation method of core-shell nanoparticles based on lipid membrane and metal organic framework
  • Preparation method of core-shell nanoparticles based on lipid membrane and metal organic framework
  • Preparation method of core-shell nanoparticles based on lipid membrane and metal organic framework

Examples

Experimental program
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Effect test

Embodiment 1

[0049] A core-shell drug carrier, in which ZIF-8 carries doxorubicin hydrochloride DOX / anti-tumor active drug as the core drug-loading model, and the surface is modified to embed Abarelix polypeptide and IR780 liposome shell. The present invention uses prostate cancer cells to test ZIF -8 targeting and tumor cell killing effect, prepared according to the following steps:

[0050] (1) Preparation of ZIF-D nanoparticles:

[0051] 1) Zinc nitrate hexahydrate and 2-methylimidazole were selected as the synthetic materials of ZIF-8, 0.786g of 2-MIM was dissolved in 0.9mL water to obtain an aqueous solution, and then 0.5 mL of an aqueous solution containing 1.6 mg DOX was added to the solution, Mix and stir for 2 minutes at 37°C and stir evenly;

[0052] 2) Add 0.1 mL of 12.6 mg of Zn (NO 3 ) 2 ·6H 2 O aqueous solution, then the mixed solution was reacted at 37°C for 15 minutes at a stirring speed of 300-800 rpm / min;

[0053] 3) After the reaction, centrifuge at 6500 rpm / min for...

Embodiment 2

[0074] A ZIF-D@ALIP drug-loading system with doxorubicin hydrochloride DOX / anti-tumor active drug as the core drug-loading model, and Abarelix polypeptide and IR780 embedded in the lipid shell as the model to test the drug-loading properties of ZIF-8 The easy modification of lipid shells is prepared according to the following steps:

[0075] A method for preparing core-shell nanoparticles based on lipid membranes and metal-organic frameworks, as follows:

[0076] (1) Preparation of ZIF-8@DOX (ZIF-D) nanoparticles:

[0077] 1) Zinc nitrate hexahydrate and 2-methylimidazole were selected as the synthetic materials of ZIF-8. 0.786g 2-methylimidazole was dissolved in 0.9 mL aqueous solution, and then 0.5 mL aqueous solution containing 1.8mgDOX was added to the solution. Mix and stir for 2 minutes at 37°C; then,

[0078] 2) Slowly add 0.1 mL of an aqueous solution containing 12.6 mg of zinc nitrate hexahydrate to the above solution, and then react the mixture at 37°C for 30 minut...

Embodiment 3

[0088] A ZIF-D@ALIP drug-loading system with doxorubicin hydrochloride DOX / anti-tumor active drug as the core drug-loading model, and Abarelix polypeptide and IR780 embedded in the lipid shell as the model to test the drug-loading properties of ZIF-8 The ease of modification of lipid shells was prepared as follows:

[0089] (1) Synthesis of ZIF-8@DOX (ZIF-D):

[0090] 1) Zinc nitrate hexahydrate and 2-methylimidazole were selected as the synthetic materials of ZIF-8, 0.8 g 2-methylimidazole was dissolved in 0.9 mL aqueous solution, and then 0.5 mL aqueous solution containing 1.6 mg DOX was added to the solution, at 37 Mix and stir at ℃ for 2 minutes; then,

[0091] 2) Slowly add 0.1 mL of an aqueous solution containing 12.6 mg of zinc nitrate hexahydrate to the above solution, and then react the mixture at 37°C with a stirring speed of 300-800 rpm / min for 60 min;

[0092] 3) After the reaction, centrifuge at 6500 rpm / min for 20 minutes to separate and remove the supernatant,...

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Abstract

The invention relates to a preparation method and application of a drug carrier. The preparation method comprises preparation of ZIF-M nanoparticles, preparation of ZIF-M@PVP/DMPC nanoparticles and preparation of ZIF-M@LIP nanoparticles. According to the invention, the main materials of the nanoparticles are ZIF-8 and lipidosome, and are low in toxicity, good in biocompatibility and good in degradation capacity, so that the problem of toxic residues in a chemical synthesis method is solved; the prepared nanoparticles have the advantages of high crystallinity, uniform particles and stable physical and chemical properties; reaction raw materials are cheap and easy to obtain, the preparation method is simple in process and high in operability, and production and application requirements can be further met; and when IR780 is embedded into the lipid shell of the nanoparticle, controllable release of the drug, namely pH and near-infrared NIR controlled release of the drug, can be realized.

Description

technical field [0001] The invention relates to a preparation method and application of a drug carrier, in particular to a method for preparing core-shell nanoparticles based on liposomes and metal organic frameworks, and the invention belongs to the field of nano drug carriers. Background technique [0002] The application of nanotechnology in people's life, medical treatment and scientific and technological development has attracted increasing attention. In particular, the new nano drug delivery system has great application potential because of its high drug loading, good targeting and low toxicity. The new nano drug delivery system refers to the drug delivery system that selectively concentrates the drug in the target tissue, target organ or internal structure of the cell by means of nano-scale carriers, ligands or antibodies; Realize the slow release of the drug to the affected area. However, there are very few nanocarriers with good biocompatibility, good targeting, re...

Claims

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Application Information

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IPC IPC(8): A61K31/704A61K41/00A61K9/52A61K47/42A61K47/22A61P35/00
CPCA61K41/0057A61K31/704A61K9/5052A61K47/22A61P35/00A61K2300/00Y02A50/30
Inventor 崔大祥徐艳张兆坤陈玮嘉朱君金彩虹
Owner SHANGHAI NAT ENG RES CENT FORNANOTECH
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