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Polypeptide flexible liposome as well as preparation method and application thereof

A liposome and peptide flexible technology, applied in the field of personal care products, can solve the problem that active peptides are difficult to exert their efficacy, and achieve the effect of increasing transdermal performance and strong deformability

Active Publication Date: 2021-04-16
SOUTH CHINA UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after the peptide enters the skin, it is easily decomposed by the enzymes in the skin, and due to its physical and chemical properties such as insoluble in water, hydrophilicity, and the drug delivery barrier function of the stratum corneum, it is difficult for the active peptide to exert its effect in the process of transdermal administration.

Method used

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  • Polypeptide flexible liposome as well as preparation method and application thereof
  • Polypeptide flexible liposome as well as preparation method and application thereof
  • Polypeptide flexible liposome as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Take 0.03g of palmitoyl pentapeptide-4, 0.2g of cholesterol, 3g of soybean lecithin and 0.2g of sodium cholate and place them in a rotary evaporator. Then add 10ml of methanol, ultrasonic and assisted by hand shaking to accelerate the dissolution until the solution turns into a clear and transparent yellow liquid. The organic solution was rotary evaporated at 40° C. for 1 h, and a light yellow film was left on the wall of the bottle at this time.

[0031] The rotary evaporator was placed in a vacuum drying oven, the temperature was set at 25° C., and the time was 1 h, and the trace amount of methanol in the film was removed by vacuum drying.

[0032] Take 90ml of aqueous solution, add 0.3g of Tween-20, mix the two evenly with ultrasound, then pour the solution into a rotary evaporator, stir in a water bath at 45°C for 0.5h to hydrate the film, and then sonicate until the bottle wall until the film is completely peeled off. At this point a pale yellow emulsion was obta...

Embodiment 2

[0035] Take 0.08g of palmitoyl pentapeptide-4, 0.3g of cholesterol, 5g of egg yolk lecithin and 0.3g of sodium deoxycholate and place them in a rotary evaporator. Then add 10ml of ethanol, and accelerate the dissolution with ultrasonication and hand shaking until the solution turns into a clear and transparent yellow liquid. The organic solution was rotary evaporated at 35° C. for 1.5 h, and a light yellow film was left on the bottle wall at this time.

[0036] The rotary evaporator was placed in a vacuum drying oven, the temperature was set at 30° C., the time was 0.5 h, and the remaining methanol in the film was removed by vacuum drying.

[0037] Take 95ml of aqueous solution, add 0.5g of Span-40, mix the two evenly with ultrasound, then pour the solution into a rotary evaporator, stir in a water bath at 40°C for 0.5h to hydrate the film, and then sonicate until the bottle wall until the film is completely peeled off. At this point a pale yellow emulsion was obtained.

[...

Embodiment 3

[0040] Take 0.05g of palmitoyl pentapeptide-4, 4g of phosphatidylcholine, 0.25g of cholesterol, 0.25g of sodium cholate, and dissolve them in 10ml of chloroform, sonicate and use hand shaking to accelerate the dissolution until the solution becomes clear and transparent yellow liquid. The organic solution was poured into a rotary evaporator, and the organic solution was rotary evaporated at 45° C. for 2 hours. At this time, a layer of light yellow film was left on the bottle wall.

[0041] The rotary evaporator was placed in a vacuum drying oven, the temperature was set at 35° C., and the time was 2 hours, and the residual chloroform in the film was removed by vacuum drying.

[0042] Take 90ml of aqueous solution, add 0.4g of Span-20, mix the two evenly by ultrasound, then pour the solution into a rotary evaporator, stir in a water bath at 30°C for 1 hour to hydrate the film, and then sonicate until it reaches the surface of the bottle wall until the film is completely peeled...

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Abstract

The invention belongs to the technical field of personal care products, and discloses a polypeptide flexible liposome, a preparation method and applications thereof. The preparation method comprises the following steps: (1) adding phospholipid, cholesterol, a membrane material softener and active polypeptide into an organic solvent, carrying out ultrasonic oscillation dissolving, placing the mixed solution into a rotary evaporation container, and carrying out rotary evaporation to remove the organic solvent so as to obtain a liposome solution, obtaining a layer of lipid film on the wall of the rotary evaporation container; (2) adding the water phase containing the surfactant into a rotary evaporation container, carrying out water bath constant-temperature stirring, and then carrying out ultrasonic treatment to enable a lipid film on the rotary evaporation container to completely fall off, so as to obtain a polypeptide flexible liposome primary emulsion; and (3) carrying out high-pressure homogenization treatment on the obtained polypeptide flexible liposome primary emulsion, and filtering the obtained homogenized solution by using a microfiltration membrane to remove impurities, thereby finally obtaining the polypeptide flexible liposome. The lipidosome is used for effectively encapsulating the polypeptide, so that the transdermal performance of the lipid vesicle is greatly improved, polypeptide molecules can enter the deeper layer of the skin to play a role, and the lipid vesicle can be applied to anti-wrinkle products.

Description

technical field [0001] The invention belongs to the technical field of personal care products, and in particular relates to a preparation method of a polypeptide-containing flexible liposome and its application in anti-wrinkle products. Background technique [0002] Skin wrinkles are caused by the joint action of endogenous and exogenous factors. Among them, exogenous factors include ultraviolet radiation, smoke and harmful chemicals, etc., and the reactive oxygen species (ROS) generated by their contact with the skin will directly damage the DNA of skin cells and the collagen, elastin or mucopolysaccharide molecules of the extracellular matrix , so that the skin becomes thinner, the basement membrane of the epidermis and dermis is damaged, the number of cells and the number of blood vessels decrease, resulting in the generation of skin wrinkles; Decreased, decreased skin hydration capacity, decreased concentration of proteoglycan and hyaluronic acid in the skin, decreased ...

Claims

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Application Information

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IPC IPC(8): A61K8/64A61K8/14A61K8/63A61K8/49A61K8/37A61K8/34A61Q19/08
Inventor 谢阳恺程建华李映伟张继勇周心慧
Owner SOUTH CHINA UNIV OF TECH
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