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Nanometer lipid ultrasonic contrast agent and preparation method thereof

A technology of ultrasound contrast agent and nano-lipid, which is applied in the direction of echo/ultrasound imaging agent, which can solve the problems of attenuation therapy and achieve the effect of low peak intensity, simple method and small particle size

Inactive Publication Date: 2014-03-19
SOUTHEAST UNIV
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Problems solved by technology

[0008] At present, the average diameter of ultrasound contrast agents in clinical use is several microns, and the vascular endothelial gap in the disease state allows particles with a diameter less than 700nm to pass through at most, so micron-sized ultrasound contrast agents cannot penetrate the vascular endothelial gap, which greatly weakens its therapeutic effect ability

Method used

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  • Nanometer lipid ultrasonic contrast agent and preparation method thereof
  • Nanometer lipid ultrasonic contrast agent and preparation method thereof
  • Nanometer lipid ultrasonic contrast agent and preparation method thereof

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preparation example Construction

[0027] 2 Preparation and detection of nano-lipid contrast agent

[0028] 2.1 Preparation of nano-lipid contrast agent

Embodiment 1

[0030] The preparation of nano-lipid contrast agent by lipid film dispersion and mechanical oscillation method comprises the following steps:

[0031] (1) Weigh distearoyl lecithin, diphenylphosphoryl azide, distearoyl phosphatidylethanolamine-polyethylene glycol 2000 according to the molar ratio of 80:12:8, mix them and place them in a container;

[0032] (2) Add the mixed solution of chloroform and methanol to the mixture prepared in step (1), the mass volume ratio of the mixture and the mixed solution is 0.0005g / ml, and the volume ratio of chloroform and methanol in the mixed solution is 2:1, and then Perform ultrasonic vibration until the above-mentioned mixed substances in the container are fully dissolved;

[0033] (3) Place the container containing the mixed substance prepared in step (2) on a rotary evaporator, and operate it at 50°C, at a speed of 100r / min, under vacuum conditions, so that the organic solvent in the container is fully volatilized until the container ...

Embodiment 2

[0037] Embodiment 2, basic process step is the same as embodiment 1, difference is as follows:

[0038] In step (1), weigh distearoyl lecithin, diphenylphosphoryl azide, distearoyl phosphatidylethanolamine-polyethylene glycol 2000 according to the molar ratio of 83:11:6 to prepare nano lipid contrast agent ;

[0039] In step (2), the mass-volume ratio of the mixture prepared in step (1) to the chloroform-methanol mixture was 0.0008 g / ml to prepare a nano-lipid contrast agent, and the volume ratio of chloroform to methanol was 1.5:1.

[0040] In step (3), the mixture obtained in step (2) was placed on a rotary evaporator, and operated at 51° C., rotating speed 80 r / min, and vacuuming.

[0041] In step (4), block polyether F-68 is added to 0.01mol / L phosphate buffer according to the mass-volume ratio of 1.5 mg / ml, and then added to the lipid-attached film obtained in step (2) After dissolving the lipid film, ultrasonically disperse to form a lipid suspension with a phospholipi...

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Abstract

The invention discloses a nanometer lipid ultrasonic contrast agent and a preparation method thereof. According to the method, the nanometer lipid ultrasonic contrast agent which is small in particle size and can develop stably is prepared by adopting lipid film dispersion and mechanical oscillation methods, is good in outer shell crushing resistance, is difficult to crack under the condition of low mechanical index, is extremely low in solubility and diffusion rate in a solution, can perform microcirculation pouring well, can last longer time in blood, has favorable acoustic property and stability, can obviously enhance the heart, kidney, liver and tumor development by in-vivo radiography, and long in radiography time which is more than 30 minutes.

Description

technical field [0001] The invention belongs to the field of nanomaterial preparation, and in particular relates to a preparation method of nano lipid ultrasonic contrast agent. Background technique [0002] Ultrasound imaging technology is a widely used, non-invasive and low-cost medical imaging method, however, ordinary ultrasound imaging without the use of contrast agents has low resolution. Compared with conventional ultrasonography, contrast-enhanced ultrasonography has the advantages of dynamic, real-time, and continuous display of organ parenchyma, lesion vascular architecture, and tissue perfusion. More importantly, compared with other methods, contrast-enhanced ultrasonography is simple, easy to repeat, cheap, and No radioactivity, no nephrotoxicity, high safety. Today, contrast-enhanced ultrasonography has been included together with enhanced CT and M in the three routine imaging diagnostic methods of the liver, and this technology has also been extended to the ap...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/22
Inventor 张东生李宏波
Owner SOUTHEAST UNIV
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