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Active detection method for concealed spore egg vesicle and Giardia sporocyst in drinking water

A technology for activity detection and cryptosporidium, applied in biochemical equipment and methods, microbial measurement/testing, fluorescence/phosphorescence, etc., can solve the problem of low accuracy, high cost, and inability to determine Cryptosporidium oocysts and Giardia Cyst activity and other issues to achieve high accuracy and reduce the probability of existence

Inactive Publication Date: 2009-05-13
HARBIN INST OF TECH
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Problems solved by technology

[0004] In order to solve the problems of high cost, low accuracy and inability to determine the activity of Cryptosporidium oocysts and Giardia cysts in existing methods for detecting Cryptosporidium oocysts and Giardia cysts in drinking water, the present invention provides A method for the detection of Cryptosporidium oocysts and Giardia cysts in drinking water

Method used

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  • Active detection method for concealed spore egg vesicle and Giardia sporocyst in drinking water
  • Active detection method for concealed spore egg vesicle and Giardia sporocyst in drinking water

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specific Embodiment approach 1

[0010] Specific embodiment one: the detection method of a kind of cryptosporidium oocyst and giardia cyst in drinking water according to the present embodiment is carried out according to the following steps: one, first filter water sample with 200~500 mesh screens, then filter the water sample with an aperture of 1.5-3.5μm filter membrane for suction filtration; 2. Put the filter membrane after the suction filtration in step 1 into a beaker, wash with 5-10mL buffer solution with a pH value of 7.2, and collect the attachments under cleaning; 3. 1. Place the attachments collected in step 2 in a centrifuge tube, centrifuge at a speed of 2000-4000r / min for 10-20min, and discard the supernatant; 4. Carry out sucrose gradient centrifugation at a speed of 1500-2500r / min Centrifuge for 5-15 minutes under the conditions of the centrifuge tube, then suck the middle layer between the two liquid surfaces in the centrifuge tube, and put it into a 1.5mL centrifuge tube; the mass concentrati...

specific Embodiment approach 2

[0014] Embodiment 2: This embodiment differs from Embodiment 1 in that in step 1, the water sample is first filtered with a 300-400 mesh screen, and then suction-filtered with a filter membrane with a pore size of 2-3 μm. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0015] Embodiment 3: This embodiment differs from Embodiment 1 in that in step 1, the water sample is first filtered with a 300-mesh screen, and then suction-filtered with a filter membrane with a pore size of 2-3 μm. Other steps and parameters are the same as those in Embodiment 1.

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Abstract

The invention relates to a method for detecting the activity of cryptosporidium cyst and giardia lamblia sporocyst in drinking water. The method solves the problem that the existing method for detecting the cryptosporidium cyst and the giardia lamblia sporocyst in the drinking water has high cost and low precision and can not confirm the activity of the cryptosporidium cyst and the giardia lamblia sporocyst. The method comprises the steps as follows: 1, water sample pretreatment; 2, elution; 3, condensation; 4 the purification of the cryptosporidium cyst and the giardia lamblia sporocyst; 5, dying; 6, microscopical examination for observing the cryptosporidium cyst and the giardia lamblia sporocyst; and the detection of the cryptosporidium cyst and the giardia lamblia sporocyst in the drinking water is realized. The method does not require complex instrument and equipment; the method is simple and easy for grasping, thereby reducing the investment on the equipment greatly and reducing the detection cost; and the method can detect whether the cryptosporidium cyst and the giardia lamblia sporocyst have the activity or not and has the high precision of detection result.

Description

technical field [0001] The invention relates to a method for detecting Cryptosporidium oocysts and Giardia cysts in drinking water. Background technique [0002] Because Cryptosporidium and Giardia pollute domestic water and drinking water, the outbreaks of related diseases have occurred in developed countries such as the United Kingdom and the United States, and have posed a serious threat to public health. Cryptosporidium oocysts and Giardia spores Cysts (referred to as "two worms") have also become one of the water-borne biological risk factors that have attracted much attention. In the newly promulgated "Drinking Water Hygienic Standards" in China, Cryptosporidium and Giardia have been used as unconventional indicators of water quality and their limit values ​​have been specified. However, according to the current drinking water treatment process and water quality in my country From the current situation, there is a high potential risk of "two worms" outbreaks. Therefore...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64C12Q1/02
Inventor 吴明松黄君礼李绍峰张玉玲高云霞
Owner HARBIN INST OF TECH
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