Method for establishing SD mouse fat and diabetes research model through diet and STZ induction

Abstract

The invention provides a method for establishing an SD mouse fat and diabetes research model through diet and STZ induction. Through high-fat feed and STZ induction, the attacking characteristics of fat and diabetes of humans are induced, and an animal model for establishing pathogenesis of fat and type-2-diabetes caused by insulin resistance and partial defects of pancreatic beta cells is provided. Mice are divided into a normal control group, a fat group and a diabetes group, and each group has six SD male mice. The high-fat feed is adopted for feeding mice for eight weeks, a pure-fat insulin resistance model is induced, after the mice are fed with the high-fat feed for eight weeks, injection of STZ small doze in batch is conducted, and establishment of the diabetes animal model is induced. The method can better simulate the attacking characteristics of human type-2-diabetes caused by improper diet and enjoinment factors currently, and compared with other modeling, the method has better scientific significance of clinical and fundamental research combination.

Description

Technical field [0001] The invention belongs to the medical field, and in particular relates to a method for the study of obesity and diabetes in SD rats induced by diet and STZ. Background technique [0002] Diabetes, especially type 2 diabetes, has become a public health problem worldwide. It is not only a metabolic disease, but also closely related to the pathogenesis of inflammatory response, immune system activation, and microvascular changes, and the resulting complications Symptoms are an independent risk factor that increases the premature death rate. Therefore, reducing and delaying the occurrence of diabetes is of great significance to medical clinical research. [0003] There are many types of diabetes models. Current research shows that SD rats, as experimental animals, have similar pathological characteristics of diabetes to humans. In the pathogenesis of type 2 diabetes, two key factors are decreased insulin sensitivity and pancreatic β-cell dysfunction. There is e...

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Problems solved by technology

Chinese patent application CN107929311A discloses a method for establishing an animal model of type 2 diabetes, which uses a normal diet, Escherichia coli lipopolysaccharide and STZ to construct the model, but the model has a single form and only uses blood sugar as a judgment standard, which is not applicable Judgment based on individual samples

In addition, this model uses gavage instead of a high-fat diet. However, gavage will cause great damage to the oral mucosa of rats, and it also has extremely high requirements for the experimenter's gavage techniques. Failure to gavage will cause rats to choke. Coughing and other discomforts, and then cause respiratory system and infectious diseases to interfere with the experimental process, and even cause the death of rats and cause the experiment to fall off

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