32results about How to "Short passage time" patented technology

The invention discloses a triple-authentication gate control system and a control method. The triple-authentication gate control system comprises an image collection module, a card reader module, a local controller module, a gate control output module, a mobile phone, a system management module and a database module. Through verifying whether an identity card is legal or not, the local controller module is triggered to be connected to Internet through 3G/GPRS, the system management module receives the login request of the local controller to generate random two-dimensional codes and time stamps which are sent into the mobile phone and the local controller module through multimedia messages and network, then, the local controller obtains two-dimensional codes through the card reader module, and compares the obtained two-dimensional codes to the two-dimensional codes downloaded from the system management module through 3G/GPRS network, next, the time stamp comparison is carried out, finally, whether all the authentication is legal or not is judged, and the gate is opened and images are collected if all the authentication is legal. The invention ensures high safety of the gate control system, has the advantages of short authentication time, high authentication accurate rate and low cost, and is favorable for large-range application and popularization.

The invention discloses a larimichthys crocea liver cell line and an establishment method thereof. The larimichthys crocea liver cell line is preserved in the China center for type culture collection (CCTCC) On October 31, 2013 and has a preservation number of CCTCC NO: C2013157. The larimichthys crocea liver cell line can realize serial passage, can provide a large amount of larimichthys crocea liver cell lines, can be used for pathogenic characteristic research, vaccine development, functional gene research and breeding research, has excellent properties, comprises fibroblast-like cells and mainly comprises cells having parapodiums stretching out and irregular shapes. The larimichthys crocea liver cell line has the advantages of exuberant division, short passage time, easy digestion, good adherence rate and hunger resistance. The establishment method has good repeatability. The larimichthys crocea liver cell line has good stability.

The roller cam follower (1) comprises a hydraulic support unit (3) and a down or lateral cam shaft of an internal combustion engine. The roller cam follower is assembled from a roller shaft (2) for the stroke operation of a piston of a fuel high pressure pump and the hydraulic support unit. An independent claim is also included for a roller shaft has a support unit which is received in sections in a recess.

A drying device for drying coated, elongated work pieces in a continuous process including a transport device for transporting the work piece. For drying an edge or surface of the work piece, heat sources are arranged in a drying chamber. In a drying zone, drying vapor is generated during drying. The vapor is removed by an airflow. The airflow is supplied via a supply channel and fans. A controller is provided to control the fans.

The invention discloses an attenuated method for fowl infectious bronchitis viruses. The method comprises the following steps: the toxic strain YX10 is inoculated on SPF chick embryos, 103.5-4EID50/0.2mL of the toxic strain is inoculated on every embryo; after cultured for certain time, the chick embryos are placed at the temperature of minus 20 DEG C for half an hour; allantoic fluids are collected in sterile conditions; the passage of the next generation is carried out; the culture temperature of the first generation to the tenth generation is 37 DEG C and the culture time is 48 h; the culture temperature of the eleventh generation to the fourteenth generation is lowered by 1 degree per generation and the culture time is 48 h; from the fifteenth generation, the culture temperature is 32 DEG C, and the culture time is 32-48 h. The method can save the passage time effectively, and reduces the loss of immunogenicity. The method is beneficial to develop novel vaccines in time and effectively.

The invention discloses a construction method and an application of a PHF6 <delta> and JAK3<M511I> double-mutation acute T lymphocytic leukemia mouse model. The preparation method comprises the following steps: utilizing a Vav1-Cre mouse to mate with a Phf6<fl/fl> mouse to obtain Vav1-Cre; a Phf6<fl/Y> (Phf6<-/Y>) male mouse; mating the Mx1-Cre mouse and the Phf6<fl/fl> to obtain Mx1-Cre; Phf6<fl/Y>male mouse; respectively enriching Lin-cells in bone marrow of two male mice, infecting retroviruses overexpressing GFP-JAK3M511I, and transplanting GFP + cells into receptor mice irradiated by a lethal dose through caudal vein injection, so as to construct two T-ALL leukemia mouse models. The model provided by the invention can be rapidly passed in mice, simulates the leukemia process of a T-ALL patient with PHF6 gene and JAK3 gene double mutation, and is used for targeted therapy drug screening and curative effect evaluation.

The invention discloses a dental pulp stem cell separation and culture method. The method comprises steps as follows: separation of dental pulp tissue, enzymolysis of the dental pulp tissue with IV type collagenase, V type collagenase and pancreatin mixed enzyme with the mass volume concentration of 0.05%-0.5%, primary culture of dental pulp stem cells and adding of cell growth factors, glutamine,decavitamin, insulin, tremella polysaccharide, a codonopsis tangshen extract, a dendrobium officinale extract and other nutritional ingredients. The method has the advantages as follows: the dental pulp tissue is more thoroughly digested, and the total number of the obtained dental pulp stem cells is larger; the total number of the obtained dental pulp stem cells is increased, the cell viabilityof the dental pulp stem cells is increased, besides, the passage time of the stem cells is shortened, and the isolated culture cycle of dental pulp stem cells is greatly shortened.

The invention discloses an intelligent traffic light capable of adjusting passing time at any moment according to waiting vehicles. The intelligent traffic light comprises a traffic light unit, a traffic light intelligent chip control system and a camera. The intelligent traffic light is characterized in that traffic crossroads can be intelligently managed through the intelligent traffic light, and the intelligent chip identifies the number of the vehicles waiting for a red light through the camera and displays the time of a green light, so that the waiting time of a driver can be reduced, the traffic pressure of the crossroads can be alleviated, the traffic can be alleviated and can be controlled completely without errors, protection is provided for the traffic vehicles, and the safety is guaranteed.

The invention relates to a highly efficient fuel gas pulsating combustor with an adjustable temperature field, which is composed of fuel gas inlet pipes, air inlet pipes, a combustion chamber, a heat releasing chamber and a furnace pipe, wherein, at least a group of fuel gas inlet pipes and air inlet pipes are arranged and connected at intervals at a tubular end of the combustion chamber, the other end of the combustion chamber is connected with the heat releasing chamber, and a plurality of branch furnace pipes are radially connected on an arc shaped end socket of the outlet of the heat releasing chamber. The diameter of the cylinder of the heat releasing chamber connected with the combustion chamber is larger than that of the combustion chamber, at least one flow flexing plate is radially arranged inside the heat releasing chamber, and isomorphic or heterogeneous pore passages are arranged on each flow flexing plate. The highly efficient fuel gas pulsating combustor with the adjustable temperature field of the invention has the advantages of high combustion efficiency, combustion intensity and heat exchange performance, low pollutant content in the discharged flue gas, long service life and improved working efficiency.

The invention provides a pomfret muscle cell line. The pomfret muscle cell line is a pomfret muscle cell PaM with the preservation number of CCTCC NO: C2021154. The pomfret muscle tissue cell line provided by the invention can be continuously passed to 50 generations and is kept stable so far, and a large number of stable pomfret muscle cells can be provided for nutrition metabolism, functional gene research, breeding research and the like. The provided cell line is excellent in character and is a fibroblast, the cell is fusiform or irregular triangle-shaped, a plurality of irregular bulges extend outwards from a cell body, and the cells are tightly arranged. The cell is short in generation time, can be cryopreserved and recovered, and and can perform various molecular biology experiments such as siRNA interference, plasmid transfection and the like.

The invention discloses a grid flocculation basin based on a fan blade structure filler, a plurality of vertically staggered grid plates are arranged in a basin body, and water passing holes are formed in the adjacent grid plates in the same direction in a staggered manner; a plurality of filler frames are arranged in the flocculation grid; fan blade fillers are mounted at the upper parts of the filler frames; the fan blade filler comprises a support formed by connecting a plurality of square pipes, an upper pressing plate arranged at the upper end of the support and a lower pressing plate arranged at the lower end of the support, through holes are formed in the upper pressing plate and the lower pressing plate, a plurality of impellers are arranged on the pin, each impeller comprises an outer cylinder, a plurality of blades arranged on the outer cylinder and an inner cylinder arranged in the outer cylinder, and the inner cylinder is fixedly installed on the pin. The fan blade filler combination is regular, the flocculation passing time is short, water flow forms tiny vortex flow in the flocculation zone, flocculation equipment in a hydraulic stirring form is arranged into grids inthe flocculation zone, vortexes are increased, the colloid collision probability and the coagulation effect are improved, the turbulence vortex effect is enhanced, and the flocculation coagulation efficiency is improved.

The invention relates to the field of chip manufacturing and processing, particularly relates to a method for preparing carbon dioxide ionized water for cleaning chips as well as a generating device thereof. The invention aims to provide a method and device for preparing online carbon dioxide ionized water, which have the advantages of simple structure, and capability of increasing the dissolution ratio of carbon dioxide gas in deionized water and effectively improving the stability of ion concentration of the prepared ionized water. The method comprises the following steps: 1) feeding deionized water (DIW) and carbon dioxide (CO2) into a gas-liquid mixing cylinder to carry out complete mixing, wherein the gas-liquid mixing cylinder is provided with a clamping gas film; 2) carrying out ionic conductivity detection on the ionized water discharged from the gas-liquid mixing cylinder, thereby obtaining the conductivity value of the ionized water; 3) comparing the obtained conductivity value of the ionized water with a preset conductivity value, and adjusting a pressure-regulating valve by using a controller so as to control the inlet capacity of the carbon dioxide; and 4) obtaining ionized water with the preset conductivity value.

The invention provides a high-frequency multi-section pressurizing hot pressing processing method. The method comprises the following steps: 1, a mold is cleaned and materials are prepared, the materials are stacked, the stacked materials are pressed into a regular and compact entity, and residues on the mold are cleaned; 2, a release agent is applied and the mold is heated, namely the mold is heated, and the release agent is applied on the surface of the mold; 3, a plurality of hot presses with different pressures are prepared on the production line, the pressures of the hot presses are gradually increased from the first hot press to the last hot press, and the pressure of the last hot press is the same as the pressure of the hot press for single-time hot pressing; 4, the heated mold is fed into a production line and sequentially passes through hot presses with different pressures. 5, cooling and demolding are conducted, specifically, the mold passing through the last hot press is subjected to water cooling to 200 DEG C through a pipeline, and the mold cooled to 200 DEG C is subjected to demolding; and 6, machining forming is conducted, a demolded product is cleaned, and polishing is conducted after cleaning. The method has the advantage that the hot-pressing effect is improved.

The invention relates to a method for culturing pig hair papilla cells. The pig hair papilla cells are taken from pig ear skin tissues, I-type collagenase and pancreatin are used for digestion and separation, and a fetal calf serum culture medium is used for adding rat tail collagen for cell culture. By adopting the method provided by the invention, the epidermis and dermis structures are easy to distinguish, the hair follicle structure is clear and easy to separate, the cell passage time is shortened by about 13 hours, and the adherence is firmer. According to the method provided by the invention, the characteristics of the pig hair papilla cells can be kept within seven generations, and the purity is high, so that the pig hair papilla cells with high purity and relatively good passage consistency can be obtained in batches.

The invention relates to a microwave broadband instant frequency measurement receiving system, which comprises a radio frequency processing unit, a power distribution unit, a signal conversion unit and a programmable gate []array unit, wherein the ratio frequency processing unit is used for receiving radio frequency signals; the radiation frequency processing unit converts the input radio frequency signals into first baseband signals to be output; the power distribution unit is used for receiving the first baseband signals and allocates the signals to form at least two paths of second basebandsignals to be output; the signal conversion unit is used for receiving each path of second baseband signals in a one-to-one corresponding way; each second base band signal is converted to the presetfrequency and is then output; the programmable gate array unit is used for receiving each second baseband signal, and after []looking-up table operation, the programmable gate array unit outputs multipath parallel frequency state code or high-speed optical signals through a driver. The frequency measurement precision of the equipment is high; the EMS being smaller than 3 MHz can be reached; the size is small; the weight is light; the power consumption is low; the passing time is short; the operation is convenient.

The invention relates to the technical field of intelligent cabinets, in particular to an automatic temperature and humidity adjusting system and method for an ACU cabinet, the system comprises an outer cabinet body, an inner cabinet body and a control assembly, the inner cabinet body is arranged in the outer cabinet body, and a circulating air duct and an air compression assembly are arranged between the inner cabinet body and the outer cabinet body; an air storage box is arranged in the circulating air duct; the air compression assembly comprises air compression equipment, a first air compression pipeline and a second air compression pipeline; one end of the first air compression pipeline is connected with air compression equipment, and the other end is connected with the inner cabinet body; the air compression equipment is connected with the air storage box; one end of the second air compression pipeline is connected with the air storage box, and the other end is connected with the inner cabinet body; the control assembly comprises a temperature and humidity detection module and a temperature and humidity adjusting module, the temperature and humidity in the ACU cabinet can be automatically adjusted, and normal work of the ACU area control module is prevented from being affected.

The invention belongs to the technical field of biology, and particularly relates to an establishment method and application of a sheep endometrial epithelial cell line. An hTERT gene is introduced into the sheep endometrial epithelial cells through a PB transposon vector, immortalization of the cells is achieved, and the sheep endometrial epithelial cell line which is easy to culture, high in growth speed and easy and convenient to operate and the establishment method of the cell line are provided. The PiggyBac transposon transgenic vector used in the invention can be inserted into a TTAA locus of a cell genome at a fixed point, so the influences of genome epigenetic modification and a position effect on a target gene can be effectively avoided, and the target gene can be stably and continuously expressed. Thus, immortalized cells can be obtained more efficiently, and the invention guarantees that the physiological characteristics of the transfected cells are closer to those of primary cells.