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Normal Temperature Preservation Method of Caproic Acid Bacteria

A caproic acid bacteria, room temperature technology, applied in the field of microorganisms, can solve problems such as easy damage, high technical equipment requirements, difficult activation, etc., to simplify the experimental operation process, save the passage time, and the experimental operation is simple and easy to operate.

Active Publication Date: 2022-04-15
HUBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the problems that caproic acid bacteria are easily damaged, difficult to activate, and have high requirements for technical equipment in low-temperature cryopreservation, the present invention provides a brand-new room temperature preservation method for caproic acid bacteria, which aims to improve the culture medium and add protection agent, the way of using oxygen scavenger, and finally realize the room temperature preservation of caproic acid bacteria

Method used

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  • Normal Temperature Preservation Method of Caproic Acid Bacteria
  • Normal Temperature Preservation Method of Caproic Acid Bacteria

Examples

Experimental program
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Effect test

Embodiment 1

[0038] 1. Take 100g of fresh pit mud, add 500ml of deionized water, mix well, let stand for 2 hours, stir once every half hour, centrifuge at 10000r / min for 10min, absorb the supernatant to obtain the pit mud extract.

[0039] 2. Put the centrifuged pit mud into an oven at 105°C for 6 hours, mechanically grind it through a 100-mesh sieve, pack it in a sealed bag, wrap the outer layer with newspaper, sterilize at 121°C for 30 minutes, take it out and place it in a constant temperature culture at 30°C Place in the box for one day, and then repeat the above operation for secondary sterilization. Take 1g of pit mud and dissolve it in 100ml of distilled water, then absorb 1ml of pit mud aqueous solution and place it on ES solid medium, spread and culture on a petri dish at 34°C for 2 days, no microorganisms grow. Pit mud strain protectant can be used.

[0040] 3. Weigh 0.5g of ammonium sulfate, 5g of sodium acetate, 0.4g of dipotassium hydrogen phosphate, 0.2g of magnesium sulfate,...

Embodiment 2

[0046] 1. Take 100g of fresh pit mud, add 500ml of deionized water, mix well, let stand for 2 hours, stir once every half hour, centrifuge at 10000r / min for 10min, absorb the supernatant to obtain the pit mud extract.

[0047] 2. Put the centrifuged pit mud into an oven at 105°C for 6 hours, mechanically grind it through a 100-mesh sieve, pack it in a sealed bag, wrap the outer layer with newspaper, sterilize at 121°C for 30 minutes, take it out and place it in a constant temperature culture at 30°C Place in the box for one day, and then repeat the above operation for secondary sterilization. Take 1g of pit mud and dissolve it in 100ml of distilled water, then absorb 1ml of pit mud aqueous solution and place it on ES solid medium, spread and culture on a petri dish at 34°C for 2 days, no microorganisms grow. Pit mud strain protectant can be used.

[0048] 3. Weigh 0.5g of ammonium sulfate, 5g of sodium acetate, 0.4g of dipotassium hydrogen phosphate, 0.2g of magnesium sulfate, 1...

Embodiment 3

[0054] 1. Take 100g of fresh pit mud, add 500ml of deionized water, mix well, let stand for 2 hours, stir once every half hour, centrifuge at 10000r / min for 10min, absorb the supernatant to obtain the pit mud extract.

[0055] 2. Put the centrifuged pit mud into an oven at 105°C for 6 hours, mechanically grind it through a 100-mesh sieve, pack it in a sealed bag, wrap the outer layer with newspaper, sterilize at 121°C for 30 minutes, take it out and place it in a constant temperature culture at 30°C Place in the box for one day, and then repeat the above operation for secondary sterilization. Take 1g of pit mud and dissolve it in 100ml of distilled water, then absorb 1ml of pit mud aqueous solution and place it on ES solid medium, spread and culture on a petri dish at 34°C for 2 days, no microorganisms grow. Pit mud strain protectant can be used.

[0056] 3. Weigh 0.5g of ammonium sulfate, 5g of sodium acetate, 0.4g of dipotassium hydrogen phosphate, 0.2g of magnesium sulfate,...

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Abstract

The invention discloses a normal temperature preservation method of caproic acid bacteria. The purpose of the present invention is to improve the culture medium, add a protective agent, and use an oxygen scavenger to finally realize the normal temperature preservation of caproic acid bacteria. The strain preservation method provided by the present invention can be applied to those laboratories with basic anaerobic culture facilities. It has low requirements on technical equipment, small investment, mild storage conditions, and is suitable for use in laboratories.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a normal-temperature preservation method for caproic acid bacteria. Background technique [0002] Microbial strain preservation technology is a basic and important technology in the field of microbiology, which is indispensable in microbiological experiments and practical applications. At present, the commonly used strain preservation methods mainly include subculture preservation method, liquid paraffin covering preservation method, carrier preservation method, host preservation method, cryopreservation method, etc. A common feature of these methods is the need for low temperature or ultra-low temperature preservation of strains. In the process of cryopreservation of strains, the metabolism of microorganisms is kept in the least active or relatively static state, so as to realize the preservation of strains for a long time. However, these low-temperature or cryopreservation me...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/04C12N1/20C12R1/01
CPCC12N1/04C12N1/20
Inventor 汪江波王浩孔博张瑞景何超徐健余汉超董超姜喜波蔡凤娇
Owner HUBEI UNIV OF TECH
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