26 results about "Pancreatinum" patented technology

The invention relates to the field of biology, and discloses a cornu cervi pantotrichum stem cell exosome preparation and a preparation method thereof.The preparation method of the cornu cervi pantotrichum stem cell exosome preparation comprises the following steps that after cornu cervi pantotrichum is disinfected, a semitransparent mesenchymal layer at the tip of the cornu cervi pantotrichum is separated out, the cornu cervi pantotrichum is cut into pieces and then flushed with PBS containing double antibodies, the cornu cervi pantotrichum is put into tissue digestion mixed enzyme, and the cornu cervi pantotrichum exosome preparation is obtained centrifuging and cleaning after digestion, and then spreading in a culture bottle for culture to obtain an isolate; when cells of the isolate grow to 70%-90% of a culture bottle, discarding an original culture solution, cleaning with PBS, adding pancreatin for digestion, terminating digestion with a complete medium, and performing cell passage to obtain a passage; and performing gene editing treatment on the cells of the passage. By obtaining the stem cells of the cornu cervi pantotrichum, damage to a donor when the stem cells are obtained by the donor is avoided, and due to the fact that the cornu cervi pantotrichum stem cells have stable biological characteristics, the difference between different sources is reduced, and gene editing is carried out on the subcultured cells.

The invention discloses a construction and evaluation method of a liver injury organoid model, which comprises the following steps: (1) selecting mouse liver cells, perfusing a mouse with pancreatin through a sterile operation technology, removing blood in the liver, collecting liver tissues, and carrying out fine enzymolysis digestion treatment to obtain a single-cell suspension; (2) inoculating the single-cell suspension into a three-dimensional culture system, and carrying out preliminary stimulation by adopting a culture medium combined with growth factors and inhibition small molecules, so as to promote the growth, proliferation and differentiation of cells in a three-dimensional space; according to the cell growth condition, subculture operation is carried out in good time, so that continuous culture and amplification of liver organs are ensured; and (3) exposing the maturely cultured liver organoid in a stimulating factor for simulating a liver injury pathological environment, and constructing different types of liver injury organoid models. According to the liver injury organ-like model construction method, model construction can be completed in a short time, the research period is greatly shortened, the efficiency is high, and the cost is low.

The invention provides chickpea protein peptide as well as a preparation method and application thereof. The preparation method of the chickpea protein peptide provided by the invention comprises the following steps: sequentially carrying out enzymolysis on chickpea protein isolate by using neutral protease, pepsin and pancreatin; after the enzymolysis is finished, performing ultrafiltration on an enzymolysis product by using an ultrafiltration membrane with the interception amount of 3kDa, and collecting components with the molecular weight of less than 3kDa; and finally, carrying out gel chromatography on the component, and collecting the corresponding component to obtain the chickpea protein peptide. The chickpea protein peptide prepared by the method has inhibitory activity on DPP-IV, alpha-glucosidase and alpha-amylase, can effectively inhibit the activity of Caco-2 cell sucrase, maltase and DPP-IV, and has a certain inhibitory effect on glucose transport; therefore, the chickpea protein peptide provided by the invention has good hypoglycemic activity, and a new thought is provided for development of food-borne functional protein peptides.

The invention discloses a culture medium for identifying gardnerella vaginalis, and relates to the technical field of bacterial culture media, the key points of the technical scheme are as follows: the culture medium comprises the following raw material components by concentration: 10.0 g / L of casein pancreatin digest, 3.0 g / L of cardiac pancreatin digest, 5.0 g / L of yeast extract powder, 1.0 g / L of corn starch, 5.0 g / L of sodium chloride and 13.0 g / L of agar, and the final concentration of defiberized sheep blood is 5%. The pharmaceutical composition is prepared from 1 mg / mL of sodium alginate, 1 mg / mL of sodium alginate, 1 mg / mL of polyanethole Sulfonate (SPS) and 10 [mu] g / mL of vancomycin. According to the invention, through optimal combination of specific components and dosages thereof, rapid identification and screening of gardnerella vaginalis can be realized, and the method has the advantages of accurate result, strong specificity and the like, and has practical popularization and application values.

The utility model discloses a pancreatin production and separation device, which relates to the technical field of separation devices and comprises a control console, a lifting mechanism is arranged at the top of the control console, a driving mechanism is arranged on the lifting mechanism and meshed with the lifting mechanism, a protective sleeve is connected onto the lifting mechanism, and the protective sleeve is sleeved on the control console. Under the action of the lifting mechanism, the driving mechanism and the protective sleeve, the ultrafiltration membrane component can be automatically cleaned, and meanwhile, cleaning water can be prevented from splashing when the ultrafiltration membrane component is cleaned, so that the ultrafiltration membrane component can be cleaned conveniently, and the cleaning efficiency of the ultrafiltration membrane component is improved. And the damage to the human body caused by splashed cleaning water is avoided, the water pipe does not need to be manually held for cleaning, the labor burden of workers is reduced, and therefore the cleaning efficiency is improved.

The present invention provides high potency pharmaceutical compositions comprising high activity pancreatin enzymes. The invention is also directed to a process of producing HA-pancreatin enzymes and its compositions or dosage forms, and methods for their use.

The invention discloses an attenuated, broad-spectrum and pancreatin-independent porcine epidemic diarrhea virus recombinant strain as well as a construction method and application thereof, and belongs to the technical field of animal virology and genetic engineering. According to the present invention, the 894-993 site amino acid sequence of the PEDV AH2012 / 12 variant strain S protein is transformed into the 894-993 site amino acid sequence of the JS2008 classical strain S protein through the CRISPR / Cas9 system and the homologous recombination method, and the recombinant virus rAH2012 / 12-894-993aaJS2008 is obtained through the rescue; compared with a parent virus AH2012 / 12, the recombinant virus rAH2012 / 12-894-993aaJS2008 has the advantages that the proliferation titer in cells is obviously improved, the toxicity is highly weakened, a cross-protective neutralizing antibody aiming at the G1 type and the G2 type of the PEDV can be induced, and the virus strain can be used for fundamental research and vaccine research and development.

The utility model relates to the technical field of pancreatin processing, in particular to pancreatin filtering equipment which comprises a base and a processing assembly, the processing assembly comprises a filter sieve, a frame body, a box body, a sliding rod, a spring, a brush, a fixed block, a pressing block, a frame body, a driving component, a fixed component and an extrusion component, the frame body is fixedly mounted on one side of the base, the extrusion component is arranged on the frame body, the pressing block is connected with the extrusion component and is in sliding connection with the frame body, the fixed component is connected with the frame body, and the fixed block is in sliding connection with the frame body. The box body is fixedly installed on the side, close to the frame body, of the base, the sliding rod is fixedly installed on the side, away from the base, of the box body, the frame body is slidably installed on the side, away from the box body, of the sliding rod, one end of the spring is connected with the frame body, the other end of the spring is connected with the box body, the brush is installed on the box body through a bolt, and the driving component is arranged on the box body. The filter sieve is detachably connected with the frame body, so that the pancreatin filtering effect is improved.

The utility model discloses degreasing equipment for pancreatin production, which is characterized in that supporting leg columns are mounted and connected on the periphery of the outer wall of the lower end of a bottom degreasing machine body, a second electromagnetic valve is arranged at a discharge port of the lower end of the bottom degreasing machine body, and a filter bag lifting mechanism is arranged on the inner side of the upper end of the bottom degreasing machine body; a first electromagnetic valve is arranged at the upper end of the filter bag lifting mechanism, a constant-temperature stirring tank body is arranged at the upper end of the first electromagnetic valve, a cleaning sealing cover is arranged at the upper end of the constant-temperature stirring tank body, and a stirring mechanism is arranged at the position, located in the constant-temperature stirring tank body, of the inner side of the cleaning sealing cover; the stirring mechanism, the first electromagnetic valve, the filter bag lifting mechanism, the bottom degreasing machine body and the second electromagnetic valve are electrically connected with an external controller through connecting wires. A filter bag lifting mechanism structure is arranged, so that a worker can conveniently take the filter cloth body for operation and use.

The invention belongs to the technical field of bioengineering, and discloses a method for constructing a bone-affinity mouse hepatoma cell line with bone metastasis characteristic, which comprises the following steps: digesting a mouse hepatoma cell line Hepa1-6 with pancreatin for 5 minutes, adding into a complete medium to stop digestion, centrifuging for 5 minutes at normal temperature by 300g, resuspending into 1 million cells / 100mu l by using PBS, and transferring into a 1.5 ml EP tube; after the mouse is anesthetized, shaving off hair at the chest of the mouse by using a shaver with the abdomen facing upwards, fixing the mouse on an operation table, sucking 100 [mu] l of cell suspension by using an insulin needle, inserting the needle from the intersection point of the left-side xiphoid process and the lower costal margin, and slowly injecting cells after active blood return is seen; the bone metastasis formation condition of the lower limb bone Hepa1-6 of the mouse is monitored regularly through an SPECTRAL Lago X small animal living body imaging system, isoflurane is used as an anesthetic, and it is guaranteed that the shooting time and the exposure condition of each time of monitoring are consistent; after obvious bone metastasis is formed, obvious mobility inconvenience of the mouse is observed; through multi-generation in-vivo screening, the mouse liver cancer cell line with strong bone affinity is obtained.

The utility model relates to the field of medical instruments, and discloses a pancreatic duct stent. The pancreatic duct stent comprises a first end, the first end is a pancreatic duct end, and the first end is provided with an end hole and a side hole; the second end is a small intestine end, and a side hole is formed in the second end; the two ends of the middle section are connected with the first end and the second end respectively, and the middle section is provided with no side hole. The pancreatic duct support has the anti-slipping, anti-blocking and anti-injury functions, and pancreatic related operative complications are effectively reduced; moreover, the middle side-hole-free section is lengthened, the length of the middle side-hole-free section is increased to 5 cm, the middle side-hole-free section can cross a biliary-intestinal anastomotic stoma, activation of pancreatin by bile near a pancreatic-intestinal anastomotic stoma is reduced, and the pancreatic leakage occurrence rate is reduced.

Methods for prophylaxis, treatment, and reduction of infection, re-infection, and transmission rates of Coronaviruses and more particularly Coronavirus Disease 2019 (COVID-19) resulting from a SARS-CoV-2 viral infection with the use of a pharmaceutical preparation comprising one or more coated or uncoated digestive enzymes, such as pancreatic enzymes and porcine pancreatic enzymes are described herein.

This invention discloses a method for dynamic regulation of pancreatic enzymes based on dietary intake, comprising the following steps: S1: collecting patient data through an input module; S2: calculating an individualized pancreatic enzyme dose based on the data collected by the input module through an algorithm module; S3: outputting the pancreatic enzyme dose and dietary recommendations based on the calculation results through an output module; S4: adjusting parameters through a closed-loop feedback module. The pancreatic enzyme dose is dynamically calculated based on the patient's fat intake and digestive capacity at each meal to achieve a precise match between "how much fat is consumed and how much enzyme is given."

1. Name of the product in this design: Packaging Box (Compound Pancreatic Enzyme Powder). 2. Purpose of this design: for product packaging. 3. The key design features of this product are the combination of pattern and color. 4. The image or photograph that best illustrates the design's key points: a 3D model. 5. The design for which protection is sought includes color.

The invention relates to biotechnology, in particular, to a method for producing an enzymatic lysate of biomass of methane-oxidizing bacteria, the method comprising enzymatic hydrolysis of a bacterial suspension using an enzyme preparation, which is derived from a pancreas, and / or pancreatin obtained from cattle or pigs, in amount of from 5000 to 7000 units of proteolytic activity per 1 kg of absolutely dry substance of the biomass of bacteria, at pH of from 7 to 8.5 and a temperature of 45°C to 55°C for from 4 to 12 hours. The invention also relates to a product obtained by said method. The resulting product has a high content of short-chain peptides with a molecular weight of from 150 to 1000 Da and can be used to produce feed additives and biologically active food supplements. The invention provides for an increase in bioavailability of intracellular bacterial components.

The invention discloses a preparation method of feed-grade coated pancreatin with high temperature resistance and acid resistance, the feed-grade coated pancreatin comprises an enzyme-containing pellet core and a coating layer, and the enzyme-containing pellet core is composed of pancreatin, sodium sulfate, microcrystalline cellulose, kaolin and starch; and the coating layer consists of mono / diglycerol fatty acid ester, hydrogenated palm oil and calcium carbonate. The particle size of the coated pancreatin ranges from 20 meshes to 60 meshes, and the particle size is uniform; compared with uncoated pancreatin powder, the pancreatin coated with the coating material has the advantages that the enzyme activity retention rate can be increased from 36.73% to 73.34% when the pancreatin is treated at 80 DEG C for 5 min, and the enzyme activity retention rate can be increased from 0.72% to 86.92% when the pancreatin is treated at pH 2.5 for 2 h. The coated pancreatin provided by the invention reduces the influence of high temperature on enzyme activity in the granulation process of poultry feed, reduces the damage of gastric juice to enzyme when poultry ingest, and can effectively improve the utilization rate of feed enzyme.

The invention discloses a preparation method of high-activity pancreatin, and relates to the technical field of biological medicine, and the preparation method comprises the following steps: crushing pig pancreas, carrying out composite enzymatic hydrolysis, and degreasing to obtain degreased pancreas pulp; adding a first activating agent to perform first-stage activation at 3-5 DEG C, then heating to 13-17 DEG C, and adding a second activating agent to perform second-stage activation; adding an ELP fusion lipase trapping agent to obtain a magnetic bead-lipase compound precipitate and a first supernatant; removing the magnetic beads from the magnetic bead-lipase compound precipitate to obtain lipase; centrifuging the first supernate to obtain ELP-trypsin compound precipitate and second supernate; carrying out ELP removal on the ELP-trypsin compound precipitate to obtain trypsin; adding PEG (Polyethylene Glycol) 6000 into the second supernate to obtain amylase; the pancreatin is separated and extracted by combining composite enzymolysis, degreasing, gradient activation and an ELP self-precipitation method, so that the activity and the yield of the pancreatin are effectively improved.

Title: An improved process for the separation and analysis of pancreatic enzymes. The present invention relates to reversed phase ultrahigh-performance liquid chromatography (RP- UHPLC) process by using C18 column at a suitable more than one gradient of mobile phase useful for the separation and characterisation of pancreatic protein mixture comprising at least one enzyme lipase, amylase, protease and variant thereof. The present invention further provides qualitative and quantitative analysis of separated Pancreatic protein mixture suitable for pharmaceutical use.

The invention discloses a treatment unit and a sterilization method for removing microorganisms, especially bacillus cereus, without affecting pancreatin activity, the treatment unit comprises a double-layer solid filter element to terminate the sterilization effect of a sterilization solution and stabilize the pH value of a system, and the sterilization method comprises the steps of microorganism inactivation, treatment by the treatment unit and drying by a forced air drying process; according to the treatment unit, through the synergistic effect of the upper-layer solid filter element and the lower-layer solid filter element, the stable PH value is maintained while the sterilization effect of the sterilization liquid is terminated, and the pancreatin solution which is free of oxidability, moderate in pH and capable of achieving the microorganism standard is obtained. In addition, through the specific working concentration of the non-compound PAA stock solution, the specific treatment unit composition and the forced air drying process and parameters, the problem that the existing sterilization method cannot give consideration to both the bacillus cereus removal effect and the pancreatin activity retention is solved.

The invention discloses long-acting high pancreatin degradation resistance lactase, a crystal ball preparation and preparation thereof, the lactase comprises a specific mutation site, and the pancreatin degradation resistance is remarkably improved. The preparation sequentially comprises a grease coating layer containing lactase pellets, a polysaccharide isolation coating layer and an enteric coating layer from inside to outside. During preparation, firstly, the lactase and the auxiliary materials are subjected to extrusion rolling and grease coating to form pellets, and then polysaccharide isolation coating and enteric coating are sequentially carried out. The preparation has good storage stability, can efficiently deliver enzyme activity in gastrointestinal tracts, and effectively alleviates lactose intolerance. The method is simple in process and suitable for industrial production.

The present invention relates to a composition comprising a solid support; a lipase or fragment thereof immobilized on the surface of the solid support, wherein the lipase or fragment thereof is in an open conformation); a protease or fragment thereof immobilized on the surface of the solid support; an amylase or fragment thereof immobilized on the surface of the solid support; an agent that interacts with a lid domain of a lipase or fragment thereof; a protective layer for protecting lipase or fragments thereof, protease or fragments thereof, and amylase or fragments thereof by embedding lipase or fragments thereof, protease or fragments thereof, and amylase or fragments thereof; and a functional component fixed on the surface of the protective layer, in which the functional component fixed on the surface of the protective layer is a polymer comprising repeating units, in which each repeating unit comprises at least one amino group and / or at least one mercapto group.

The invention discloses a preparation method and application of a cyclic peptide compound, CTC resin is taken as a carrier, Fmoc-Gly-OH, Fmoc-Lys (Boc)-OH, Fmoc-His (Trt)-OH, Fmoc-Gly-OH, Fmoc-Lys (Boc)-OH and Fmoc-His (Trt)-OH are sequentially coupled through solid phase synthesis, and peptide resin is obtained; the preparation method comprises the following steps: cutting to obtain a straight-chain crude peptide, carrying out cyclization reaction to generate a full-protection cyclopeptide, and finally, carrying out deprotection and purification to obtain the cyclopeptide compound. The cyclopeptide compound is low in cytotoxicity, the serum stability is remarkably improved, pancreatin can be slowly degraded into GHK to play a long-acting role, fibroblast I-type collagen expression is promoted, the SOD and GSH levels under the oxidation pressure are improved, the anti-oxidation and repair effects are better than those of GHK, and the cyclopeptide compound can be applied to the fields of cosmetics, skin repair and anti-inflammatory related medicines.

The invention belongs to the technical field of biology, and particularly relates to a method for separating zebrafish juvenile fish hearts. According to the method for separating the heart of the juvenile zebrafish, the heart of the juvenile zebrafish is located on the outer side relative to visceral tissue, the heart is wrapped by a layer of pericardium, the pericardium can be decomposed by trypsin for a specific time to expose the heart, then suction is conducted through external force, and the heart is separated. The heart is separated from the juvenile fish under the action of pancreatin and external force. By means of the separation method, the integrity of the separated zebra juvenile fish heart can be kept, the separation method is rapid and efficient, and a large number of juvenile fish hearts can be separated and dissected at the same time.

The utility model discloses a pancreatin sample adding gun which comprises a shell, a piston button is installed in the center of the top end of the shell, a sealing column is installed at the bottom end in the shell, a first cavity is formed in the sealing column, first springs are installed at the bottom ends of the two sides of the sealing column, and the other sides of the first springs are connected with lock hooks. A screw is arranged in the middle of the lock hook, a first detaching button is installed on the side, close to the shell, of the lock hook, a detachable air column is installed at the bottom of the sealing column, a connecting opening is formed in the top end of the detachable air column, a sealing ring is arranged on the outer surface of the connecting opening, and grooves are formed in the two sides of the outer portion of the detachable air column. A suction nozzle is connected to the center of the bottom of the detachable air column, the detachable air column can be separated from the gun body by pressing the first detachable button, cleaning and disinfection are facilitated, two sets of second air holes are formed in the edge of the top end of the suction nozzle, and an isolation hood is arranged at the top end of the suction nozzle to achieve the anti-splashing effect.

Methods for prophylaxis, treatment, and reduction of infection, re-infection, and transmission rates of Coronaviruses and more particularly Coronavirus Disease 2019 (COVID-19) resulting from a SARS-CoV-2 viral infection with the use of a pharmaceutical preparation comprising one or more coated or uncoated digestive enzymes, such as pancreatic enzymes and porcine pancreatic enzymes are described herein.

The invention discloses a compound protease composition and application thereof. The compound protease composition comprises pancreatin, bromelain and papain, and each protease is wrapped by lipidosome to respectively obtain pancreatin lipidosome, bromelain lipidosome and papain lipidosome. The protease of the compound protease composition can stably exist, does not degrade each other, and can permeate into the skin to play a role, so that the compound protease composition has a wide application prospect in the fields of cosmetics and personal care.