Serum-free suspension domestication method of Vero cells
A technology for serum-free, serum-free media used in cell culture. It can solve the problems of limited number of proliferation and amplification, difficult cultivation process, and limited attachment area, and achieve the effect of convenient amplification of the cultivation process and large number of proliferation and amplification
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[0014] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention will be further elaborated below.
[0015] Serum-free suspension acclimatization method of Vero cells,
[0016] Step 1: When the Vero cells are cultured on the wall until the confluency reaches 80%, the supernatant is discarded, and D-PBS without calcium and magnesium ions is added for washing. In step 1, the disposable cell culture square flask with product number T-75 produced by Shanghai Yuanpei Biotechnology Co., Ltd. was used for adherent culture. After discarding the supernatant, add 5ml of D-PBS without calcium and magnesium ions and wash twice. The D-PBS without calcium and magnesium ions is preferably the D-PBS without calcium and magnesium ions produced by Shanghai Yuanpei Biotechnology Co., Ltd. with the product number B210KJ.
[0017] Step 2: Discard D-PBS, add trypsin, shake at room temperature for a w...
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