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Method for quickly separating Schistosoma japonicum immature egg

A schistosome and immature technology, applied in the field of separation of immature eggs of Schistosoma japonicum, can solve the problems of low purity of eggs and long separation time, and achieve the effects of high purity, improved yield and improved work efficiency

Inactive Publication Date: 2007-10-17
汪世平 +1
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the shortcomings of long separation time and low purity of the obtained eggs in the prior art, and provide a method for rapidly separating immature eggs of Schistosoma japonicum

Method used

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  • Method for quickly separating Schistosoma japonicum immature egg
  • Method for quickly separating Schistosoma japonicum immature egg

Examples

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Embodiment 1

[0016] Example 1: Research on the Rapid Isolation Method of Schistosoma japonicum Immature Eggs

[0017] Using the abdominal smear method, each New Zealand rabbit was infected with 2000-2500 cercariae of Schistosoma japonicum, and was fed under clean and ventilated conditions. 32 days after infection, animals were sacrificed by cutting the carotid artery, the liver was taken out and placed in a sterilized container, the bile ducts, blood vessels and connective tissue were quickly removed, the blood was quickly washed with water, the liver was cut into pieces, and an appropriate amount of water was added, and poured into The tuned high-speed homogenizer was used to quickly mash 3 times at 10000r / min, each time for 1 minute, with an interval of 3 minutes, and the liver tissue homogenate was obtained and placed in a clean vessel.

[0018] The homogenate was passed through 80-mesh, 160-mesh, 260-mesh, and 320-mesh silk sieves successively, and the filter residue in the 320-mesh si...

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Abstract

The invention discloses a method of fast separating Japan schistosome unfledged spawn, which employs technique of combining repeat sieving and pancreatin processing and completes in two hours. Purity of the obtained spawn is above 99%, structure of the spawn is integrated and good biological activity is remained, which can satisfy basic need of antigen extraction and analysis. Comparing to exist technique, yield of the spawn is largely increased.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a method for separating immature eggs of Schistosoma japonicum. Background technique [0002] Schistosomiasis is a widely distributed and serious zoonotic parasitic disease. Its prevalence has seriously affected the health of the people and hindered the economic development of the affected areas. Schistosomiasis eggs play an important role in the pathological damage and spread of schistosomiasis, and are the key factors causing histopathological damage and pathogen transmission. Therefore, the focus of schistosomiasis control is ultimately how to control the source of infection of schistosomiasis. Whether it is the research on on-site control countermeasures based on controlling the source of infection, or the laboratory research on schistosomiasis eggs, it is necessary to separate high-purity and biologically active intact schistosomiasis eggs. [0003] The method for ext...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/34C12N5/06C12N5/07
Inventor 汪世平周松华何卓刘雪琴
Owner 汪世平
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