Method for culturing kidney cancer organ

A culture method and organoid technology, applied in the field of kidney cancer organoid culture, to achieve the effect of improving accuracy

Inactive Publication Date: 2021-11-30
深圳明澳生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, due to the strong heterogeneity of tumors between different types of renal cancer and among different patients of t

Method used

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  • Method for culturing kidney cancer organ
  • Method for culturing kidney cancer organ
  • Method for culturing kidney cancer organ

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Embodiment Construction

[0047] The technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present application. Obviously, the described embodiments are only some of the embodiments of the present application, not all of them. Based on the embodiments in this application, all other embodiments obtained by those skilled in the art without making creative efforts belong to the scope of protection of this application.

[0048] The terms "first" and "second" in this application are used to distinguish different objects, not to describe a specific order. Furthermore, the terms "include" and "have", as well as any variations thereof, are intended to cover a non-exclusive inclusion. For example, a process, method, system, product, or device that includes a series of steps or modules is not limited to the listed steps or modules, but optionally also includes steps or modules that a...

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Abstract

The invention discloses a method for culturing a kidney cancer organ. The method comprises the following steps: placing small kidney cancer tissue blocks in an inhibitor containing type II collagenase, and carrying out first digestion treatment to obtain a first digested substance; placing the first digested substance in a pancreatin substitute containing an inhibitor, and carrying out second digestion treatment to obtain a second digested substance; sequentially carrying out first neutralization treatment, filtering treatment and centrifugal treatment on the second digested substance to obtain single-cell precipitate; placing the single-cell precipitate in a mixed substrate of a preset culture medium and substrate glue for resuspension treatment to obtain a gel suspension containing all cells; and culturing the gel suspension according to a preset scheme, thereby obtaining the kidney cancer organ. According to the scheme, the accuracy of an expected treatment result can be improved.

Description

technical field [0001] The present application relates to the technical field of organoid culture, in particular to a method for culturing kidney cancer organoids. Background technique [0002] Renal carcinoma is a malignant tumor originating from the urinary tubular epithelial system of the renal parenchyma, and is one of the most common urinary system tumors. According to the different pathological types, renal cancer mainly includes three subtypes: clear cell renal cell carcinoma (ccRCC), papillary renal cell carcinoma (pRCC) and chromophobe renal cell carcinoma (renal cell carcinoma). carcinoma, crRCC). Kidney cancer is estimated to affect 300,000 people worldwide and kill more than 100,000 annually, with a steady increase in new cases in many countries and regions, including the United States and Europe. [0003] The clinical treatment of RCC varies with tumor stage and pathological type. Nephron-sparing surgery or radical nephrectomy can be used for patients with ea...

Claims

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Application Information

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IPC IPC(8): C12N5/071C12N5/09
CPCC12N5/0693C12N5/0686C12N2513/00C12N2509/00
Inventor 余磊陈洁琳吴万军余建辉
Owner 深圳明澳生物科技有限公司
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