Synovial sarcoma organoid culture method and medium, and transplant and use thereof
A technology for synovial sarcoma and culture medium, applied in the field of biomedicine, can solve the problems of limited diversity, small number of synovial sarcoma, and difficulty in constructing animal models of synovial sarcoma in large quantities, so as to promote in vitro growth and improve the success rate of culture. Effect
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Embodiment 1
[0065] This example is used to illustrate the culture of synovial sarcoma organoids.
[0066] (1) Acquisition of synovial sarcoma tissue samples
[0067] The surgically excised or punctured tissues of patients with synovial sarcoma were used as synovial sarcoma tissue samples, which were stored in pre-cooled synovial sarcoma protective solution and transported to the operating room within 48 hours at 4°C.
[0068] (2) Acquisition of synovial sarcoma tissue cells
[0069] The synovial sarcoma tissue sample was placed in a sterile petri dish, washed twice with PBS at 4°C, and then an enzymatic hydrolysis solution was added, wherein 0.1 ml of the enzymatic hydrolysis solution was added to 1 mg of the synovial sarcoma tissue sample. Using sterile instruments, the synovial sarcoma tissue samples were cut into tissue blocks with a diameter of less than 1 mm, and then the culture dish was placed in an incubator at 37°C for enzymatic hydrolysis for 0.5 to 2 hours to obtain an enzymat...
Embodiment 2
[0079] This example is used to illustrate the application of synovial sarcoma organoids in the construction of PDX mouse model.
[0080] For the culture product obtained in Example 1, a synovial sarcoma organoid with a diameter of about 0.2 mm was taken out together with a matrigel layer within a thickness of 0.2 mm around it to obtain a transplant.
[0081] Mice (SPE grade female SCID mice, 4 weeks old, purchased from Beijing Weitong Lihua Laboratory Animal Co., Ltd.) were anesthetized with 0.1 ml / 20 g of sodium pentobarbital. After successful anesthesia, the mice were placed on a clean operating table, and the underarm skin of the mice was incised with sterile instruments, and a 3-5 mm deep incision was made on the muscle layer of the mice with ophthalmic forceps. Then gently pull up the muscle layer, and use ophthalmic forceps to pick up the treated graft and put it into the incision. The ophthalmic forceps were loosened, the muscle layer was smoothed, and the mouse skin w...
Embodiment 3
[0084] The synovial sarcoma organoids were cultured according to the method of Example 1, except that the concentration of valproic acid in the separation medium used in this example was 2.4 mmol / L, and the concentration of valproic acid in the expansion medium was 0.8 mmol / L.
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