Method for culturing upper urinary tract urothelial cell carcinoma organoids

A culture method and epithelial technology, applied in the field of organoid culture

Inactive Publication Date: 2021-12-03
深圳明澳生物科技有限公司
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Problems solved by technology

[0004] However, since UTUC is a relatively rare tumor of the genitourinary system, the use of chemotherapy drugs in clinical practice is often based on inherited empirical prescriptions or personal treatment experience of doctors, and cannot be used for the use of chemotherapy drugs and the development of upper urinary tract urothelial tumors. Personalized Therapy Delivers Reliable Clinical Data

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  • Method for culturing upper urinary tract urothelial cell carcinoma organoids
  • Method for culturing upper urinary tract urothelial cell carcinoma organoids
  • Method for culturing upper urinary tract urothelial cell carcinoma organoids

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[0047] The following will clearly and completely describe the technical solutions in the embodiments of the application with reference to the drawings in the embodiments of the application. Apparently, the described embodiments are only some of the embodiments of the application, not all of them. Based on the embodiments in this application, all other embodiments obtained by those skilled in the art without making creative efforts belong to the scope of protection of this application.

[0048] The terms "first" and "second" in this application are used to distinguish different objects, not to describe a specific order. Furthermore, the terms "include" and "have", as well as any variations thereof, are intended to cover a non-exclusive inclusion. For example, a process, method, system, product, or device that includes a series of steps or modules is not limited to the listed steps or modules, but optionally also includes steps or modules that are not listed, or optionally inclu...

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Abstract

The invention discloses a method for culturing upper urinary tract urothelial cell carcinoma organoids. The method comprises the following steps: placing small upper urinary tract urothelial cell carcinoma tissue blocks in an inhibitor containing type II collagenase for first digestion treatment to obtain a first digest; placing the first digest in a pancreatin substitute containing an inhibitor, and performing second digestion treatment to obtain a second digest; sequentially performing first neutralization treatment, filtering treatment and centrifugal treatment on the second digest to obtain single cell precipitate; placing the single cell precipitate in a mixed substrate of a preset culture medium and substrate glue for resuspension treatment to obtain a gel suspension containing all cells; and culturing the gel suspension according to a preset scheme to obtain the upper urinary tract urothelial cell carcinoma organoids. According to the scheme, reliable clinical data can be provided for use of chemotherapeutic drugs and personalized treatment of upper urinary tract urothelial cell tumors.

Description

technical field [0001] The present application relates to the technical field of organoid culture, in particular to a method for culturing organoids of upper urinary tract urothelial carcinoma. Background technique [0002] Upper Urinary Tract Urothelial Cell Carcinoma (UTUC) is a rare tumor of the genitourinary system that originates from the urothelial lining of the upper urinary tract. UTUC accounts for 5-10% of all urothelial carcinomas and 10% of renal cancers. Despite the low incidence of UTUC, it is characterized by high mortality and recurrence rates, 60% of cases are aggressive at the time of diagnosis, the 5-year survival rate of patients with pT2 / pT3 invasion of the muscular wall is less than 50%, and pT4 The 5-year survival rate of patients is less than 10%. [0003] For patients with advanced upper tract urothelial carcinoma, radical nephroureterectomy combined with bladder cuff resection is still the standard treatment for UTUC, systemic chemotherapy and neoa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09C12N5/071
CPCC12N5/0693C12N5/0684C12N2513/00
Inventor 余磊陈洁琳吴万军余建辉
Owner 深圳明澳生物科技有限公司
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