Method for culturing upper urinary tract urothelial cell carcinoma organoids

A culture method and epithelial technology, applied in the field of organoid culture
CN113736736AInactive Publication Date: 2021-12-03深圳明澳生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
深圳明澳生物科技有限公司
Publication Date
2021-12-03
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for culturing upper urinary tract urothelial cell carcinoma organoids. The method comprises the following steps: placing small upper urinary tract urothelial cell carcinoma tissue blocks in an inhibitor containing type II collagenase for first digestion treatment to obtain a first digest; placing the first digest in a pancreatin substitute containing an inhibitor, and performing second digestion treatment to obtain a second digest; sequentially performing first neutralization treatment, filtering treatment and centrifugal treatment on the second digest to obtain single cell precipitate; placing the single cell precipitate in a mixed substrate of a preset culture medium and substrate glue for resuspension treatment to obtain a gel suspension containing all cells; and culturing the gel suspension according to a preset scheme to obtain the upper urinary tract urothelial cell carcinoma organoids. According to the scheme, reliable clinical data can be provided for use of chemotherapeutic drugs and personalized treatment of upper urinary tract urothelial cell tumors.
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Description

technical field

[0001] The present application relates to the technical field of organoid culture, in particular to a method for culturing organoids of upper urinary tract urothelial carcinoma. Background technique

[0002] Upper Urinary Tract Urothelial Cell Carcinoma (UTUC) is a rare tumor of the genitourinary system that originates from the urothelial lining of the upper urinary tract. UTUC accounts for 5-10% of all urothelial carcinomas and 10% of renal cancers. Despite the low incidence of UTUC, it is characterized by high mortality and recurrence rates, 60% of cases are aggressive at the time of diagnosis, the 5-year survival rate of patients with pT2 / pT3 invasion of the muscular wall is less than 50%, and pT4 The 5-year survival rate of patients is less than 10%.

[0003] For patients with advanced upper tract urothelial carcinoma, radical nephroureterectomy combined with bladder cuff resection is still the standard treatment for UTUC, systemic chemotherapy and neoa...

Claims

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