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Preparation method of pancreatic enzyme powder

A technology of pancreatin powder and pancreatin, which is applied in the direction of pancreatin, can solve the problems of great influence on enzyme activity, hidden dangers of production safety, and damage to workers' health, and achieve high three-enzyme activity, low production cost, and improved production safety Effect

Inactive Publication Date: 2014-02-12
马忠仁 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The traditional pancreatic enzyme extraction process mainly adopts organic solvent precipitation method and salting-out method, and the solvents used such as acetone and ether are volatile and flammable; the chemicals such as ammonium sulfate and sodium sulfate used cause environmental pollution, resulting in high production costs and high product yields. The rate is low, which has a great impact on the enzyme activity, and there are serious safety hazards in production, which will cause great damage to the health of workers.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A kind of preparation method step of trypsin powder of the present invention is as follows:

[0027] (1) Collection: After the cattle are slaughtered in accordance with Islamic law, the pancreas is collected within 30 minutes, and the collected pancreas is soaked in 0.125mol / L dilute sulfuric acid for 10 minutes and then preserved. The purpose of soaking in dilute sulfuric acid is to prevent enzyme loss. Live, prevent pancreas autolysis, and affect the activity and yield of the three enzymes;

[0028] (2) Preservation: store the fresh bovine pancreas in (1) at -18°C;

[0029] (3) Thawing: Take out the bovine pancreas preserved in (2), and thaw the bovine pancreas at 5°C. After the bovine pancreas is thawed, further remove impurities such as fat and connective tissue;

[0030] (4) Grinding: Grinding the beef pancreas in (3) with a meat grinder and weighing;

[0031] (5) Extraction: Add 25% ethanol (cow pancreas: 25% ethanol mass ratio = 1:1) to (4) as the extractant, a...

Embodiment 2

[0038] A kind of preparation method step of trypsin powder of the present invention is as follows:

[0039] (1) Collection: After the cattle are slaughtered in accordance with the Islamic law, the pancreas is collected within 30 minutes to prevent autolysis of the pancreas and affect the activity and yield of the three enzymes; the collected pancreas is treated with 0.125mol / L Preserve after soaking in dilute sulfuric acid for 10 minutes;

[0040] (2) Preservation: store the fresh bovine pancreas in (1) at -15°C;

[0041] (3) Thawing: Take out the bovine pancreas preserved in (2), and thaw the bovine pancreas at 2°C. After the bovine pancreas is thawed, further remove impurities such as fat and connective tissue;

[0042] (4) Grinding: Grinding the beef pancreas in (3) with a meat grinder and weighing;

[0043] (5) Extraction: add 25% ethanol (cow pancreas: 25% ethanol mass ratio = 1:1) to (4) as the extractant, and add 0.1% calcium chloride to the pancreas Activator, addin...

Embodiment 3

[0050] A kind of preparation method step of trypsin powder of the present invention is as follows:

[0051] (1) Collection: After the cattle are slaughtered in accordance with the Islamic law, the pancreas is collected within 30 minutes to prevent autolysis of the pancreas and affect the activity and yield of the three enzymes; the collected pancreas is treated with 0.125mol / L Preserve after soaking in dilute sulfuric acid for 10 minutes;

[0052] (2) Preservation: store the fresh bovine pancreas in (1) at -20°C;

[0053] (3) Thawing: take out the bovine pancreas preserved in (2), and thaw the bovine pancreas at 8°C. After the bovine pancreas is thawed, further remove impurities such as fat and connective tissue;

[0054] (4) Grinding: Grinding the beef pancreas in (3) with a meat grinder and weighing;

[0055] (5) Extraction: add 25% ethanol (cow pancreas: 25% ethanol mass ratio = 1:1) to (4) as the extractant, and add 0.2% calcium chloride to the pancreas Activator, add p...

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PUM

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Abstract

The invention provides a method for preparing pancreatic enzyme powder. The method comprises the following steps of (1) collecting pancreas; (2) preserving the pancreas at a low temperature; (3) carrying out thawing, impurity removing and mincing; (4) extracting the pancreas so as to obtain pancreas slurry, and filtering by utilizing a frame filter press, so as to obtain an extracting solution; (5) adding ethanol into the extracting solution, standing, filtering by utilizing the frame filter press, and collecting a filter cake; (6) washing and centrifuging the filter cake to obtain sediments; (7) drying the sediments in vacuum and crushing, so as to obtain the pancreatic enzyme powder. According to the method, the bovine pancreatic enzyme powder accordant with a Mohammedanism rule is provided, and the market blank is made up; a method for preparing the industrial pancreatic enzyme powder by virtue of pig pancreas is technically improved. The method has the advantages that the production cost is low, the pollution to a final product and the environment is avoided, and the production security is greatly improved; the operation process is simplified, the consumed time is short, the time for preparing each batch of product only lasts for 45-48 hours, and the method is easy to industrialize. The product prepared by utilizing the method has the advantages that three enzyme activities are high and the yield reaches 11.0%.

Description

technical field [0001] The invention belongs to the technical field of biological pharmacy and food processing, and in particular relates to a preparation method of pancreatin powder. Background technique [0002] Pancreatin is widely found in the pancreas of pigs, cattle, sheep and other animals, and its main components are trypsin, pancreatic amylase and pancreatic lipase. It is a digestive aid drug recorded in the pharmacopoeias of many countries in China and the world, and it is also the main raw and auxiliary material in the biopharmaceutical and food industries. However, at present, pancreatin is mainly extracted from pig pancreas in the domestic and foreign markets, which does not comply with Islamic law and is halal. The lack of pancreatin powder has seriously restricted the development of drugs and food products suitable for the Muslim group. Gansu has the advantage of rich pancreas resources and good pancreatin production raw materials in line with Islamic law. If ...

Claims

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Application Information

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IPC IPC(8): C12N9/94
CPCC12N9/94
Inventor 李明生冯玉萍乔自林冯若飞令世鑫马忠仁靳冬武
Owner 马忠仁
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