Preparation method of pancreatic enzyme powder
A technology of pancreatin powder and pancreatin, which is applied in the direction of pancreatin, can solve the problems of great influence on enzyme activity, hidden dangers of production safety, and damage to workers' health, and achieve high three-enzyme activity, low production cost, and improved production safety Effect
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Embodiment 1
[0026] A kind of preparation method step of trypsin powder of the present invention is as follows:
[0027] (1) Collection: After the cattle are slaughtered in accordance with Islamic law, the pancreas is collected within 30 minutes, and the collected pancreas is soaked in 0.125mol / L dilute sulfuric acid for 10 minutes and then preserved. The purpose of soaking in dilute sulfuric acid is to prevent enzyme loss. Live, prevent pancreas autolysis, and affect the activity and yield of the three enzymes;
[0028] (2) Preservation: store the fresh bovine pancreas in (1) at -18°C;
[0029] (3) Thawing: Take out the bovine pancreas preserved in (2), and thaw the bovine pancreas at 5°C. After the bovine pancreas is thawed, further remove impurities such as fat and connective tissue;
[0030] (4) Grinding: Grinding the beef pancreas in (3) with a meat grinder and weighing;
[0031] (5) Extraction: Add 25% ethanol (cow pancreas: 25% ethanol mass ratio = 1:1) to (4) as the extractant, a...
Embodiment 2
[0038] A kind of preparation method step of trypsin powder of the present invention is as follows:
[0039] (1) Collection: After the cattle are slaughtered in accordance with the Islamic law, the pancreas is collected within 30 minutes to prevent autolysis of the pancreas and affect the activity and yield of the three enzymes; the collected pancreas is treated with 0.125mol / L Preserve after soaking in dilute sulfuric acid for 10 minutes;
[0040] (2) Preservation: store the fresh bovine pancreas in (1) at -15°C;
[0041] (3) Thawing: Take out the bovine pancreas preserved in (2), and thaw the bovine pancreas at 2°C. After the bovine pancreas is thawed, further remove impurities such as fat and connective tissue;
[0042] (4) Grinding: Grinding the beef pancreas in (3) with a meat grinder and weighing;
[0043] (5) Extraction: add 25% ethanol (cow pancreas: 25% ethanol mass ratio = 1:1) to (4) as the extractant, and add 0.1% calcium chloride to the pancreas Activator, addin...
Embodiment 3
[0050] A kind of preparation method step of trypsin powder of the present invention is as follows:
[0051] (1) Collection: After the cattle are slaughtered in accordance with the Islamic law, the pancreas is collected within 30 minutes to prevent autolysis of the pancreas and affect the activity and yield of the three enzymes; the collected pancreas is treated with 0.125mol / L Preserve after soaking in dilute sulfuric acid for 10 minutes;
[0052] (2) Preservation: store the fresh bovine pancreas in (1) at -20°C;
[0053] (3) Thawing: take out the bovine pancreas preserved in (2), and thaw the bovine pancreas at 8°C. After the bovine pancreas is thawed, further remove impurities such as fat and connective tissue;
[0054] (4) Grinding: Grinding the beef pancreas in (3) with a meat grinder and weighing;
[0055] (5) Extraction: add 25% ethanol (cow pancreas: 25% ethanol mass ratio = 1:1) to (4) as the extractant, and add 0.2% calcium chloride to the pancreas Activator, add p...
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