Method for preparing antioxidant peptide by utilizing pancreatin to hydrolyze rice residue

Abstract

The invention discloses a method for preparing antioxidant peptide by utilizing pancreatin to hydrolyze rice residue. The antioxidant peptide is obtained by adopting rice residue, namely a byproduct of aginomoto production as a raw material, concentrating the rice residue to extract rice residue protein by virtue of an pickling method, hydrolyzing the rice residue protein by adopting pancreatin, centrifuging, hyperfiltering and freeze drying the rice residue, the enzymolysis condition is as follows: after the rice residue protein is mixed with water at a mass-to-volume ratio of (1:6)-(1:9), the pH value is adjusted to 7.5 to 8.0 by adding hydrochloric acid, and the pancreatin which accounts for 6-8wt% of the rice residue protein is added at the temperature of 45-55 DEG C to enzymolyze the rice residue protein for 5-6 hours. The production cost is low, the process is simple, environmental friendliness is realized, the obtained antioxidant peptide has the characteristics of reasonability in ammonia acid constitution, low irritability, safety, reliability and the like, the content of total phenols is 34.47mg / g, the IC50 value of DPPH free radical and hydroxyl free radical is respectively 2.27mg / mL and 2.98mg / mL, and the elimination capacity is good, so that the antioxidant peptide is strong in antioxidant activity and a functional food base material with potential.

Description

technical field

[0001] The invention belongs to the technical field of food processing, and relates to a method for preparing antioxidant active peptides by using trypsin to hydrolyze rice dregs, in particular to a method for preparing antioxidant active peptides by using rice dregs, a by-product of monosodium glutamate production, as a raw material and using trypsin enzymolysis method product. Background technique

[0002] Rice dregs is a by-product of rice saccharification in industrial production, and is considered to be a protein resource with good development potential. According to statistics, in the production process of monosodium glutamate and glucose, about 0.5 tons of wet rice dregs can be produced after saccharification of each ton of rice, and more than 20,000 tons of rice dregs can be produced every quarter in southern China. Almost all the protein of rice is retained in rice dregs, and its protein content is as high as 600 g / kg (dry basis), which is significa...

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