Mouse ovarian granulosa cell separation, culture and in-vitro injury model construction method
A granulosa cell and cell culture technology, applied in the field of cell culture, can solve the problems of poor understanding of the maintenance of ovarian granulosa cells after passage, apoptosis of ovarian granulosa cells, etc., to improve the yield and avoid the effects of influence.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0057] The PMSG hormone was injected into 3-4 week-old mice by intraperitoneal injection, and 48 hours later, the abdominal cavity of the mice was opened to expose bilateral ovary tissues. Remove the mouse ovarian tissue with sterile surgical scissors, wash the ovarian tissue with PBS, and remove the adipose tissue and connective tissue around the ovarian tissue. The ovarian granulosa cells were released by puncturing the follicles with a 1 mL syringe needle under a stereomicroscope. After puncturing the follicles, the ovarian tissue was digested in 0.25% trypsin for 30 minutes, and then the digestion was terminated. The two were combined into a 15 mL centrifuge tube and centrifuged at 1000 rpm for 5 minutes. After removing the supernatant, resuspend the cells with the complete medium of ovarian granulosa cells, and then inoculate them in 12-well or 6-well plates at 37°C, 5% CO 2 cultured in an incubator. After 48 hours, the original medium was discarded, and fresh complete ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com