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Pure natural cerebroprotein hydrolysate raw material preparation method

A cerebroprotein hydrolyzate, pure natural technology, applied in the field of raw material preparation of pure natural cerebroprotein hydrolyzate, can solve the problems that the fingerprint similarity cannot reach 90% standard, the clarity is unqualified, etc.

Active Publication Date: 2014-10-15
HUNAN LINUO BIOLOGICAL PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, if the amino acid is not "supplemented", qualified products cannot be produced, mainly because the fingerprint similarity cannot reach the 90% standard, and the injection products have floc and tyrosine precipitation during storage. Unqualified clarification due to substance etc.

Method used

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  • Pure natural cerebroprotein hydrolysate raw material preparation method
  • Pure natural cerebroprotein hydrolysate raw material preparation method
  • Pure natural cerebroprotein hydrolysate raw material preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The pure natural cerebroprotein hydrolyzate raw material preparation method comprises the following steps:

[0033] a. To purify the protein, take 50kg of frozen fresh pig brain, soak it in water to thaw naturally, then wash it with water, then add 100kg of purified water and use a colloid mill to homogenize it for 15 minutes, pour the slurry obtained from the homogenization into an extraction tank, heat it to 80°C and keep it warm After 15 minutes, add 250 ml of concentrated hydrochloric acid with a concentration of 12mol / L, stir evenly, cool to 40°C, centrifuge at a speed of 3000r / min, remove the upper emulsion, collect the precipitate, and obtain the precipitated cerebroprotein;

[0034]b. Pepsin enzymatic hydrolysis, add 50kg of purified water to the precipitated protein obtained in the protein purification step, homogenate for 15min, pour into the extraction tank, heat up to 30°C, then add 500g of pepsin and stir for 15min, then add 375ml concentration Stir for 12 ...

Embodiment 2

[0041] The pure natural cerebroprotein hydrolyzate raw material preparation method comprises the following steps:

[0042] a. To purify protein, take 50kg of frozen fresh pig brain, add water to soak and thaw naturally, then wash with water, then add 200kg of purified water and use a colloid mill to homogenize for 15 minutes, pour the slurry obtained from the homogenization into an extraction tank, heat to 100°C and keep it warm After 30 minutes, add 100 ml of concentrated hydrochloric acid with a concentration of 12mol / L, stir evenly, cool to 50°C, centrifuge at a speed of 4000r / min, remove the upper emulsion, collect the precipitate, and obtain the precipitated cerebroprotein;

[0043] b. Pepsin enzymatic hydrolysis, add 150kg of purified water to the precipitated protein obtained in the protein purification step, homogenate for 15min, pour it into the extraction tank, heat up to 50°C, then add 1000g of pepsin and stir for 10min, then add 450ml to a concentration of Stir wit...

Embodiment 3

[0050] The pure natural cerebroprotein hydrolyzate raw material preparation method comprises the following steps:

[0051] a. To purify protein, take 50kg of frozen fresh pig brain, add water to soak and thaw naturally, then wash with water, then add 150kg of purified water and use a colloid mill to homogenize for 15 minutes, pour the slurry obtained from the homogenization into an extraction tank, heat to 70°C and keep it warm After 10 minutes, add 200 ml of concentrated hydrochloric acid with a concentration of 12mol / L, stir well, cool to 10°C, and then centrifuge at a speed of 3500r / min, then remove the upper emulsion, collect the precipitate, and obtain the precipitated cerebroprotein;

[0052] b. Pepsin enzymatic hydrolysis, add 100kg of purified water to the precipitated protein obtained in the protein purification step, homogenate for 15min, pour it into the extraction tank, heat up to 40°C, then add 800g of pepsin and stir for 10min, then add 350ml to a concentration of...

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Abstract

The invention discloses a pure natural cerebroprotein hydrolysate raw material preparation method. The method comprises the steps as follows: a, protein purification, to be specific, a cryopreserved fresh pig brain is defrosted, cleaned, homogenized, and subjected to centrifugal separation for obtaining precipitated brain protein; b, pepsin enzymolysis, to be specific, pepsin is added in the precipitated brain protein and stirred, then concentrated hydrochloric acid is added and stirred, and then the centrifugal separation carried out, so that a pepsin enzymatic hydrolysate is obtained; c, acid hydrolysis, to be specific, sulfuric acid is added into the pepsin enzymatic hydrolysate for hydrolyzing, so that an acid hydrolysis liquid is obtained; d, trypsin enzymolysis, to be specific, the pH value is adjusted to 7-9, and pancreatin with the weight 0.1% to 0.4% of that of the pig brain is added for hydrolyzing, and the centrifugal separation is carried out, so that a pancreatin enzymatic hydrolysate is obtained; e, chromatography, to be specific, the pH value is adjusted to 6.5-7.5, and resolving is carried out by using ammonia water, so that a chromatography liquid is obtained; f, concentration, to be specific, the vacuum concentration is carried out, the pH value is adjusted to 6.5-7.5, and then frozen storage is carried out; g, impurity removing, to be specific the condensed frozen liquid is defrosted, and then subjected to centrifugal separation as well as ultrafiltration for obtaining natural cerebroprotein hydrolysate raw material.

Description

technical field [0001] The invention relates to a raw material preparation method of pure natural cerebroprotein hydrolyzate. Background technique [0002] Pure natural cerebroprotein hydrolyzate is extracted from pig brain, without protein, and contains a variety of essential amino acids, non-essential amino acids, polypeptides, etc., and has broad medicinal prospects. In the 1970s, Cerebrolysin hydrolyzate was first sold by the Austrian EBEWE Pharmaceutical Factory under the trade name of Cerebrolysin (Shipushan), which was used for the treatment of various diseases such as infantile hypoplasia, senile dementia, sequelae of cerebrovascular disease and adjuvant treatment, and achieved good results. The effect has been affirmed by clinicians. However, the preparation method of the preparation was not disclosed in all literature data at that time. In 1995, the Ministry of Health approved the production of cerebroprotein hydrolyzate injection. However, the nitrogen content ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/01A61K35/30A61P25/28A61P9/10
Inventor 钏助胜宋彦坤耿华靖彭亚聪彭长福
Owner HUNAN LINUO BIOLOGICAL PHARMA
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