Fermentation production method for amino acid

A production method and amino acid technology, applied in the direction of fermentation, etc., can solve the problems affecting the yield and quality of amino acid extraction, the inability of microorganisms, the loss of nitrogen elements, etc., and achieve the effects of eliminating unstable quality, increasing corporate profits, and reducing impurities

Inactive Publication Date: 2019-06-28
内蒙古阜丰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although corn steep liquor is rich in proteins and peptides, most of them exist in the form of macromolecular compounds, which cannot be fully utilized by microorganisms, resulting in the loss and waste of nitrogen
At the same time, proteins and peptides that are not fully utilized affect the yield and quality of amino acid extraction

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0021] According to one embodiment of the present invention, an amino acid fermentation production method of the present invention uses corn steep liquor enzymatic hydrolyzate instead of corn steep liquor and soybean meal hydrolyzate in the fermentation medium to ferment and produce amino acids. In this embodiment, the corn steep liquor enzymatic hydrolyzate completely replaces the corn steep liquor and soybean meal hydrolyzate in the fermentation medium.

[0022] According to one embodiment of the present invention, the preparation step of corn steep liquor hydrolyzate comprises:

[0023] S1. Place the corn steep liquor at a temperature of 40-60°C, and add protease, wherein the amount of protease is 0.2%-1% of the corn steep liquor quality. Through the above settings, the dosage of protease is within the range of 0.2%-1% of the mass of corn steep liquor, which not only ensures sufficient enzymatic hydrolysis of corn steep liquor, but also has a small content of protease in th...

Embodiment 1

[0029] The enzymatic hydrolyzate of corn steep liquor is prepared through the preparation steps of enzymatic hydrolyzate of corn steep liquor in the foregoing embodiments. In this embodiment, a certain amount of corn steep liquor is taken, and a single-component protease is added to the corn steep liquor. The protease is an acidic protease, and the amount of the acidic protease is 0.5% of the mass of the corn steep liquor. The enzymatic hydrolysis of corn steep liquor was carried out under the enzymatic hydrolysis temperature of 45°C. During the enzymolysis, the corn steep liquor was stirred at a speed of 30r / min, and the enzymolysis time was set at 20h, so as to obtain the enzymatic hydrolyzate of corn steep liquor after the enzymolysis.

[0030] The obtained corn steep liquor hydrolyzate is used in the glutamic acid fermentation medium, then the formula of this medium is: starch hydrolysis sugar 50g / L, corn steep liquor hydrolyzate 50ml / L, molasses 8ml / L, dipotassium hydroge...

Embodiment 2

[0034]The enzymatic hydrolyzate of corn steep liquor is prepared through the preparation steps of enzymatic hydrolyzate of corn steep liquor in the foregoing embodiments. In this embodiment, get a certain amount of corn steep liquor, add protease in corn steep liquor, this protease is composite protease, and wherein, the consumption of acidic protease is 0.4% of corn steep liquor quality, the consumption of alkaline protease is corn steep liquor quality 0.4% 0.2%. The enzymatic hydrolysis of corn steep liquor was carried out under the enzymatic hydrolysis temperature of 45°C. During the enzymolysis process, the corn steep liquor was stirred at a speed of 30r / min, and the enzymolysis time was set as 20h, so as to obtain the corn steep liquor enzymatic hydrolyzate after the enzymolysis.

[0035] The obtained corn steep liquor hydrolyzate is used in the glutamic acid fermentation medium, then the formula of this medium is: starch hydrolysis sugar 50g / L, corn steep liquor hydroly...

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PUM

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Abstract

The invention relates to a fermentation production method for amino acid. A corn steep liquor enzymatic hydrolysate is adopted for replacing corn steep liquor and a bean pulp hydrolysate in a fermentation culture medium, and fermentation production of the amino acid is achieved. The corn steep liquor in the microbial fermentation culture medium is subjected to enzymolysis into micro-molecule polypeptide and free amino acid through protease, the micro-molecule polypeptide and the free amino acid are more easily absorbed and used by microorganisms, and accordingly a nitrogen source which meets the demand of microbial fermentation is obtained. Therefore, the corn steep liquor enzymatic hydrolysate is used for replacing the corn steep liquor for feeding, so that the bean pulp hydrolysate doesnot need to be added in the fermentation process, the factor of the unstable quality of the outsourcing bean pulp hydrolysate can be eliminated, the utilization rate of nutrient substances in the cornsteep liquor can also be increased, resource waste is reduced, impurities in the amino acid extraction process are also reduced, the method helps to increase the amino acid yield and improve the amino acid quality, the production cost of an enterprise is lowered, and the enterprise benefit is improved.

Description

technical field [0001] The invention relates to the technical field of biological fermentation engineering, in particular to an amino acid fermentation production method. Background technique [0002] Amino acid fermentation needs carbon source, nitrogen source, phosphorus source, other trace elements and growth factors, etc. Among them, the nitrogen source is an essential component for the synthesis of metabolites such as proteins and amino acids. In the amino acid fermentation medium of the existing production process, the nitrogen source is often added to the medium in the form of soybean meal hydrolyzate, yeast extract, protein powder, corn steep liquor, liquid ammonia and ammonium sulfate. Corn steep liquor is concentrated from soaking liquid produced by soaking corn during starch manufacturing by wet process. It contains a large amount of soluble protein, polypeptide and other nutrients. It is widely used in microbial fermentation production mainly to provide nitrogen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/08C12P13/14
Inventor 李学朋季新利王峰王伟庄严卢松伏广好许传娟徐亚娟李文全
Owner 内蒙古阜丰生物科技有限公司
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