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Method for building mouse model with multiple-generation obesity and fatty liver pathologic change

A fatty liver and mouse technology, applied in the field of mouse model establishment, can solve problems such as inapplicable obesity and fatty liver intergenerational impact research

Inactive Publication Date: 2012-06-20
WUHAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the past 40 years, scholars have used various methods to establish different fatty liver models. However, these models are not suitable for the study of the transgenerational effects of obesity and fatty liver

Method used

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  • Method for building mouse model with multiple-generation obesity and fatty liver pathologic change
  • Method for building mouse model with multiple-generation obesity and fatty liver pathologic change
  • Method for building mouse model with multiple-generation obesity and fatty liver pathologic change

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] This example takes C57BL / 6 mice as an example to illustrate the method of the present invention to establish a mouse model in which multi-generation high-fat food feeding accelerates obesity and fatty liver lesions.

[0031] Experimental materials: C57BL / 6 mice were purchased from (Animal Experiment Center of Wuhan University); mouse feed was purchased from (Wuhan Xianglong Feed); high-fat feed was mouse feed with fat added to account for 60% of the total calories of the food.

[0032] experimental method:

[0033]The C57BL / 6 mice used in this experiment were bred by the Experimental Animal Center of Wuhan University. The 4-6 week old C57BL / 6 offspring (female and male mice) fed normal food to dams were fed with normal (NC) and high-fat food (HF) respectively, that is, NC F0 (normal 1st generation) and HF F0 group (first generation of high fat). Among them, female mice were bred with male mice fed with normal food after being fed with different foods for one month, an...

Embodiment 2

[0036] Example 2: Transgenerational effects of high-fat diet on C57BL / 6 mice

[0037] All the male mice in the experimental groups were fed with different foods for 3 months, and their physiological indexes and fatty liver pathological indexes were evaluated. To study the transgenerational effects of obesity degree, insulin resistance and liver disease in this model. The index system that the present invention adopts:

[0038] Detection of various physiological characteristics of experimental mice (including body weight, blood sugar, liver weight and liver weight / body weight); HE staining method was used to evaluate the degree of hepatic steatosis in each experimental group according to certain standards; Activation levels of somatic genes in the liver.

[0039] 1. Transgenerational effects of high-fat diet on various physiological indicators of C57BL / 6 mice

[0040] After being fed with high-fat food and normal food for 3 months, the body weight and body length of mice in ...

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Abstract

The invention discloses a method for building a mouse model with multiple-generation obesity and fatty liver pathologic change. The method comprises the steps of: respectively feeding mice with normal food (NC) and high-fat food (HF) to obtain F0-generation mice, namely an NCF0 group and an HFF0 group; feeding female mice with different foods until sexual maturity, breeding with male mice fed with normal food to obtain F1-generation mice, namely an NCF1 group and an HFF1 group; after ablactation, respectively and continuously feeding the two groups of mice with normal food and high-fat food, respectively feeding female mice with normal food and high-fat food until sexual maturity, breeding with male mice fed with normal food to obtain F2-generation mice, namely an NCF2 group and an HFF2 group; and after ablactation, respectively and continuously feeding the NCF2 group and HFF2 group of mice with normal food and high-fat food. The specific modeling route is shown in Figure 1. The result shows that the obesity degree, insulin resistance degree and fatty liver pathologic change degree of the mice fed with high-fat food are progressively increased generation by generation.

Description

technical field [0001] The invention relates to the establishment of a mouse model, specifically a method for establishing a multi-generation high-fat food feeding accelerated obesity and fatty liver disease mouse model, and the established mouse model can be used to study the effects of high-fat food on the liver and obesity intergenerational impact. Background technique [0002] Overweight and obesity have become a public health issue of great concern around the world. Two-thirds of adults in the United States are overweight or obese, and more than half of adults in Europe and other developed countries are overweight or obese. The Chinese used to be a thin group. With the development of society and economy, people's physical activities or heavy labor have been significantly reduced, and the number of overweight and obese people in China has the tendency to catch up with Western countries rapidly. The National Nutrition and Health Survey in 2002 showed that 184 million p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02
Inventor 郑凌李炯李建双黄进黄昆
Owner WUHAN UNIV
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