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Modeling method of depression animal model of pubescent mouse

An animal model, a technology of depression, applied in the field of medicine and biology, can solve the problems of different treatment strategies and ineffective treatment of depression in adolescents, and achieve the effect of simple operation

Pending Publication Date: 2018-11-13
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Moreover, recent clinical investigations have also found that some clinically commonly used drugs for the treatment of depression in adults have no effect on the treatment of depression in adolescents. This also suggests that depression in adolescence is different from depression in adulthood. May differ in pathogenesis and treatment strategies

Method used

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  • Modeling method of depression animal model of pubescent mouse
  • Modeling method of depression animal model of pubescent mouse
  • Modeling method of depression animal model of pubescent mouse

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The modeling method of puberty mouse depression animal model, it comprises the following steps: SPF class just weaned male C57BL / 6J mice (14 ± 2 grams, 21 days old) (provided by Experimental Animal Center of Southern Medical University, experimental animal breeding In a 12 / 12-hour light-dark environment (lights on at 8:00 am / lights off at 20:00 pm), free to eat and drink.)

[0031] (1) SPF grade just-weaned male C57BL / 6J mice (14 ± 2 g, 21 days old) were selected for single-cage feeding (one per cage), water and feed were freely available, and normal biological rhythms were maintained (morning 8 Turn on the lights at :00, turn off the lights at 20:00 in the evening), the breeding environment is kept quiet, and there is no communication with the outside world. The mouse cages used for breeding are non-transparent to ensure that the experimental mice cannot see other mice.

[0032](2) Continuous feeding in a single cage for 4 weeks to obtain an animal model of depression...

Embodiment 2

[0034] The detection method of puberty mouse depression animal model, it comprises following experiment:

[0035] (1) Experimental grouping: 15 adolescent depression mice modeled according to the method of Example 1 above were selected, and 15 mice not modeled (normally reared, 5 / cage) were selected as the normal control group.

[0036] (2) Detection of depression indicators:

[0037] 1. Sugar water consumption experiment:

[0038] Operation process: 24 hours before the start of the experiment, the experimental mice and the mice in the control group were housed in single cages and treated with water and food deprivation. Before the start of the sugar water consumption test, the drinking water bottle was replaced with two 100ml bottles A and B with rubber stoppers and drinking tubes and placed in the A and B positions respectively, among which, the A bottle was filled with 60ml of 1% sugar water, and the B bottle was filled with Put in 60ml of ordinary drinking water, weigh a...

Embodiment 3

[0063] The animal model of depression in adolescent mice is used for the screening of antidepressants, which includes the following experiments:

[0064] (1) Experimental grouping: select 48 pubertal depression model mice that are obtained by the method of the above embodiment 1, and are divided into normal saline group (12 mice), fluoxetine group (12 mice), imipramine group (12 mice) mice), nortriptyline group (12 mice), the above model mice were given intraperitoneal injection of normal saline group (0.1ml), fluoxetine group (20mg / kg, 0.1ml), imipramine group (30mg / kg , 0.1ml), nortriptyline group (30mg / kg, 0.1ml), injected continuously for four weeks, and continued to maintain a single cage feeding state during the administration. At the same time, 12 unmodeled mice (normally fed, 5 / cage) were selected as normal control group.

[0065] (2) Animal behavior testing:

[0066] 1. Sugar water consumption experiment:

[0067] Operation process: 24 hours before the start of the...

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Abstract

The invention discloses a modeling method of a depression animal model of a pubescent mouse. According to the current situation of stay-at-home children in today's society and the pathogenesis mechanism of depression, it is verified through a large number of experiments that a weanling male mouse of a C57BL / 6J strain is raised through a single cage, and the depression animal model of the pubescentmouse is established on the periphery. It is shown through experimental results that the mouse of a model group produces depression-like phenotypes, including animal behaviors, corticosterone and sleeping situation, so that the modeling of the depression animal model of the pubescent mouse succeeds. The model is simple in operation, the current situations of the stay-at-home children can be verywell simulated, and the blank of depression animal models at the key pubescent development stage is also filled. Through the model, the modeling method can be very well used for screening anti-pubescent depression drugs and has the important application value in pathogenesis mechanism of pubescent depression in future.

Description

Technical field: [0001] The invention belongs to the field of medical biology, and in particular relates to a method for making an animal model of depression in adolescent mice. Background technique: [0002] More than 1 / 3 of the people in the world suffer from mental illness. Depression is the most common mental illness, and its prevalence has surpassed cardiovascular and cerebrovascular diseases and tumors, ranking first in developed countries. Depressed mood, anhedonia, usually accompanied by sleep disturbance, lack of interest in work or daily life, and even suicide (more than 90% of suicides stem from depression) are the main clinical manifestations of depression, which bring endless pain to the patient himself and his family. suffering, seriously endangering human life and health. Therefore, depression has become a serious public health problem with its high incidence, high recurrence rate and suicidal tendency. [0003] The prevention and treatment of depression is...

Claims

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Application Information

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IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 高天明刘吉红杨建明尤强龙李泽林吴定宇
Owner SOUTHERN MEDICAL UNIVERSITY
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