45 results about "Sperm counts" patented technology

Test kits for assessing male fertility include a sample holder defining an object plane, a lens, and a two dimensional light sensor defining an image plane arranged along a common linear axis. The distance between the object plane and the image plane may be no more than 50 mm, and may be no more than 30 mm. A lens aperture may have an area of 1-10 mm2. The test kit may have a housing with a maximum linear dimension of no more than 100 mm. Processing circuitry may be provided that is configured to produce a sperm count and / or sperm motility measurements by processing image data from the two-dimensional light sensor.

Novel sequence tagged sites (STSs), probes and primers useful, e.g., for detecting the presence or absence of an STS in a sample, and methods of using these STSs, probes and primers, e.g., in methods of detecting alterations in the Y chromosome are disclosed. These compositions are also useful in methods of diagnosing or aiding in the diagnosis and / or cause of reduced sperm count and in methods of predicting or aiding in the prediction of the likelihood of success of infertility treatments.

A sperm-producing decoction is disclosed and warms and invigorates kidney yang. The decoction is used for patients with male infertility caused by insufficiency of the kidney yang, low sperm count, azoospermia, or a sperm amount lower than a normal value, and low sperm motility. A traditional Chinese medicine composition is prepared from following raw material medicines by weight: prepared rehmannia root, Chinese wolfberry, raspberry, common dodder, pulp of dogwood fruit, fruit of magnoliavine, semen plantaginis, epimeddium and morinda root. The sperm-producing decoction has good effects for azoospermatism, oligospermia, and normal sperm count type infertility, and is good in effects and quick-acting in curative effects for male infertility. Natural traditional Chinese components are free of side effects for human bodies. The sperm-producing decoction has functions of strengthening sperms and effectively increasing the amount of sperms.

The invention relates to a culture solution for bovine IVF and a method for improving bovine IVF. The method for bovine IVF comprises the following steps: washing a mature cumulus-oocyte complex in afertilization culture solution, transferring the mature cumulus-oocyte complex into the fertilization culture solution, and putting the fertilization culture solution into an incubator for later use;taking a frozen seminal tubule from liquid nitrogen, performing unfreezing in a water bath at 37 DEG C, injecting seminal fluid into a centrifuge tube containing a seminal fluid preparation culture solution, and discarding a supernatant after centrifugation; adding the seminal fluid preparation culture solution into the centrifuge tube, resuspending sperm precipitate, and taking a proper sperm suspension for sperm counting; and adding the sperm suspension with the calculated volume into fertilization culture liquid drops containing oocytes, placing a culture tray into the incubator, and carrying out sperm-ovum co-incubation to complete IVF operation for embryo in-vitro culture. The fertilization culture solution and the seminal fluid preparation culture solution contain sodium pyruvate, heparin and other components. The method can significantly improve the efficiency of bovine IVF.

In a new pharmaceutical combination, the herb, Vitex agnus-castus (chasteberry), enhances hormone balance by increasing progesterone release and, therefore, ovulation frequency. The antioxidants, green tea, vitamin E, and selenium, improve overall reproductive health. L-arginine, an amino acid, stimulates the reproductive organs by improving circulation. Folic acid, vitamins B6 and B12, iron, zinc and magnesium help promote womens' fertility. Sperms are highly susceptible to free radical or oxidative damage from environmental toxicants and natural aging. Vitamins C and E, coenzyme Q10 and selenium are all potent antioxidants that help improve sperm counts and quality. Ferulic acid, an antioxidant found in Dong quai, also improves sperm quality. Zinc and B vitamins (B6, B12 and folate) are critical nutrients in male reproductive systems for hormone metabolism, sperm formation and motility. The amino acid, L-carnitine, promotes formation of healthy sperm.

Test kits for assessing male fertility include a sample holder defining an object plane, a lens, and a two dimensional light sensor defining an image plane arranged along a common linear axis. The distance between the object plane and the image plane may be no more than 50 mm, and may be no more than 30 mm. A lens aperture may have an area of 1-10 mm2. The test kit may have a housing with a maximum linear dimension of no more than 100 mm. Processing circuitry may be provided that is configured to produce a sperm count and / or sperm motility measurements by processing image data from the two-dimensional light sensor.

The object of the present invention is to provide a composition comprising a Withania somnifera extract. Another object of the invention is to provide a method for increasing male sperm count in-vivo with a composition comprising Withania somnifera extracts.

The invention provides a sperm motion track reconstruction and classification method, which comprises the following steps of performing background modeling to obtain a background image; reconstructingthe sperm motion track and counting the moving sperms to obtain the motion track of each numbered sperm and the total number of the moving sperms; classifying the motional sperms to obtain a motionalsperm classification trajectory diagram and the number of PR forward motional sperms and NP non-forward motional sperms; and counting the immobile sperms, namely marking the immobile sperms in the background binarized image and counting the immobile sperms. Compared with the prior art, the recognition accuracy is improved, and errors of human factors are reduced, and labor force is saved.

Test kits for assessing male fertility include a sample holder containing at least one sample chamber, a laser light source, and a light detector to detect scattered light intensity from the sample chamber. The sample holder may include multiple sample chambers connected by sperm swim channels. The test kit may have a housing with a maximum linear dimension of no more than 100 mm. Processing circuitry may be provided that is configured to produce a sperm count and / or sperm motility measurements by processing data from scattered light intensity measurements.

Methods and systems are provided for diagnosing male infertility relating to inadequate production of phosphatidic acid and are complementary to the routine tests, assessing sperm count, motility, viability, head morphology, and white blood cell count. Additional therapeutic methods, compositions and dosage forms are provided for treating male infertility that is related to inadequate production of phosphatidic acid. Such therapeutic approaches involve the use of phosphatidic acid or at least one of its precursors in the sperm intracellular signaling pathway.

The present invention provides a method for treating infertility which may be caused by sperm immobility, comprising administering to a person in need of treatment for infertility an effective amount of a pharmaceutical composition comprising a RAR retinoid selective compound to activate sperm motility, together with a pharmaceutically acceptable excipient, and thereby providing sperm with improved motility for treating infertility.

A nutraceutical composition containing (i) garden cress, (ii) nigella saliva, (iii) commiphora myrrha, and (iv) fenugreek for treating male infertility and a method of treating symptomatic reproductive indicators of male infertility such as sperm concentration, sperm count, and progressive sperm motility by administering an effective amount of the nutraceutical composition having an effective proportion of each constituent.

The present invention relates the function of Dickkopf 3 (Dkk3 ) in testis using shRNA mediated knock down model. Specifically, the present invention provides knock down model comprising reduction in Dkk3 activity. The knock down model of Dkk3 consisting of non human vertebrates which have, incorporated in their genome, shRNA construct targeting mammalian Dkk3 gene exhibits low testis weight, low sperm count and has low litter size. The knock down model displays disrupted seminiferous tubules and are subfertile. The present invention model has a role in sex determination as its interruption leads to sex reversal of XY gonads, converting males to females. Sex reversal role of Dkk3 knock down model can find its utility in agricultural applications. The present invention describes Dkk3 as a dual function protein which is associated with sex determination as well as is essential for the process of spermatogenesis. Such testicular functional studies are useful as a model for various disease states of infertility or subfertility and for identifying a potential treatment to overcome idiopathic infertility.

The invention discloses feed for breeding cattle, belonging to the field of breeding feed. The feed is prepared from the following raw materials: bean pulp, corn flour, herbage, epimedium herb, semencuscutae, fructus lycii, fragrant-flowered garlic, bone meal, fructus crataegi and an additive, wherein the additive is prepared from oil meal and pancreatic powder. The feed for the breeding cattle provides balanced nutrition for the breeding cattle which frequently mate, can remarkably enhance the physique of the breeding cattle, promotes the gonepoiesis of the breeding cattle, increases the ejaculatory amount and sperm count of the breeding cattle, and improves the semen quality of the breeding cattle, thus improving the reproductive capacity of the breeding cattle.

Methods and compositions are provided which employ a silencing element that, when ingested by a plant insect pest, such as Coleopteran, Hemiptera, or Lepidopteran plant pest, including a Diabrotica, Leptinotarsa, Phyllotreta, Acyrthosiphan, Bemisia, Halyomorpha, Nezara, or Spodoptera plant pest, decrease the expression of a target sequence in the pest. Disclosed are various target polynucleotides set forth in any one of SEQ ID NOS: 1-53 or 107-254 disclosed herein, or variants and fragments thereof, or complements thereof, wherein a decrease in expression of one or more of the sequences in the target pest controls the pest and pest population by insect sterilization (i.e., male and female sterility, reduction of sperm count, egg production and viability) and mating-based sterile insect technique (SIT). Plants, plant parts, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.

The invention relates to a method for culturing and cryopreserving bovine in-vitro fertilization embryos. The method comprises the following steps of: washing a mature cumulus-oocyte complex in a fertilization culture solution, transferring the mature cumulus-oocyte complex into the fertilization culture solution, and putting the mature cumulus-oocyte complex into an incubator for later use; taking a frozen sperm tubule from liquid nitrogen, unfreezing, taking a sperm suspension, and counting sperms; adding the sperm suspension into fertilization culture solution drops containing oocytes to co-incubate the sperms and the oocytes, completing in-vitro fertilization operation, completely removing granular cells around the embryos by using an oocyte stripping needle, putting the embryos into an embryo culture solution for culturing, transferring available embryos into a freezing solution, and putting the embryos into liquid nitrogen for cryopreservation; and when the cryopreserved embryos need to be used, taking the tubule from the liquid nitrogen, transferring the tubule into an unfreezing solution, putting the embryos into small drops of the embryo culture solution for culturing, and completing the unfreezing process. The invention further relates to the used freezing solution and the used unfreezing solution. According to the method, the survival rate of the bovine in-vitro fertilization embryos after cryopreservation and unfreezing can be remarkably increased.

Male infertility due to reduced sperm count has received dramatically increased scientific attention in recent years, particularly the effect of excessive testicular heat on sperm motility. Currently all products designed for testicular cooling are prohibitively cumbersome, or potentially toxic. While ice can be applied directly to the testicles, this invention provides and a convenient and comfortable alternative that does not exist today: underwear specifically designed to be fitted with a freezable gel pack, permitting users to keep moving around. Researchers increasingly believe that this sort of cooling can have a positive effect on sperm health, perhaps providing another alternative to other expensive and invasive fertility treatments.

The invention relates to a drug for improving male sperm count and motility, preparations, application and a preparation method thereof. The drug and its preparations are made of pecan kernels, Siraitia grosvenorii, zinc gluconate and mannan. Auxiliary materials are added into the medicines to prepare pharmaceutical preparations according to conventional preparation processes. Application of the drug and its preparations provided by the invention to enhancement of male sperm count and motility and improvement of sperm quality can achieve unexpected therapeutic effects. Therefore, the drug and its preparations provided by the invention can be another choice for treatment of male infertility.

The invention discloses semen diluent convenient to count duck sperms and an application method of the semen diluent. The semen diluent is composed of the following components: 40 to 60g of NaHCO3 (sodium bicarbonate), 5 to 15mL of 35% of formaldehyde, 8 to 12g of gentian violet, 5 to 15mL of 96% of alcohol, and normal saline added to 1000mL. The semen after being diluted by the diluent is capable of not only quickly killing the sperms, but also improving definition when a microscopic examination is carried out, so that quantity of the duck sperms is accurately counted, and density of the drake sperms is detected, thereby providing an important reference on use of the drakes, dilution, storage and transportation of semen.

Methods and systems are provided for diagnosing male infertility relating to inadequate production of phosphatidic acid and are complementary to the routine tests, assessing sperm count, motility, viability, head morphology, and white blood cell count. Additional therapeutic methods, compositions and dosage forms are provided for treating male infertility that is related to inadequate production of phosphatidic acid. Such therapeutic approaches involve the use of phosphatidic acid or at least one of its precursors in the sperm intracellular signaling pathway.

The invention relates to a breeding feed for stock ram. The breeding feed for stock ram comprises the following raw materials: 50-60 parts of corn, 30-35 parts of silage sorghum stems, 20-22 parts of soybean powder, 15-20 parts of radish leaves, 10-18 parts of great burdock stems and leaves, 4-6 parts of cnidium fruits, 2-3 parts of curculigo rhizome, 8-10 parts of dodder seeds, 2-5 parts of Artemsia dracunculus L., 3-5 parts of convolvulate asiabell roots, 0.002-0.005 part of ferrous sulfate, 1-1.5 parts of expansive soil, 1.8-2.2 parts of potassium iodide, 2-5 parts of Indian bread mycelium, 0.5-0.8 part of L-acidum asparticum calcium, 0.002-0.005 part of ammonium sulfate and 0.02-0.025 part of vitamin B1. The feed of the invention is capable of improving immunity and reproductive capacity of the stock ram, so that genetic potential of the stock ram is normally functioned; the feed of the invention is also capable of obviously improving mating ability and sperm count of the stock ram.

A new composition and method are described for male fertility therapy. In one particular embodiment, the composition comprises Lepidium meyenii, carnitine and Coenzyme Q10. When it is administered to males as fertility therapy following the recommended therapeutic regimen, enhanced sperm count, sperm quality, and sperm motility results.

The present artificial insemination instrument may include a single use, hollow, elongate, metal pipette and a single disposable syringe that is not toxic to bovine sperm. The apparatus is used with any type of diluent that is not toxic to bovine sperm. In various embodiments, a technician may use non-sorted reduced sperm count semen straws from a bull stud, cut an unsorted semen straw into several pieces to use one piece at a time per cow, thaw one straw and mix the non-sorted semen with diluent, or use reduced sperm count semen straws filled with sex sorted semen by the manufacturer. A procedure is disclosed to use the artificial insemination instrument, reduced sperm count semen from any of the aforementioned sources, a non-toxic syringe, and a diluent to achieve conception rates at least as good as conventional prior art device and procedure conception rates.

The invention relates to a culture fluid used for bovine in vitro fertilization and a method for improving bovine in vitro fertilization. The method for bovine in vitro fertilization comprises the following steps: washing the mature cumulus-oocyte complex in the fertilization medium, then transferring it to the fertilization medium, putting it into an incubator, and putting it into an incubator for subsequent use; tube, thawed in a water bath at 37°C, inject the semen into the centrifuge tube containing the semen preparation culture medium, centrifuge and discard the supernatant; add the semen preparation culture medium to the above centrifuge tube, resuspend the sperm pellet, and take an appropriate sperm suspension for spermatogenesis Counting; add the calculated volume of sperm suspension into the fertilization culture drop filled with oocytes, and put the culture plate into the incubator to incubate the sperm and eggs together, and complete the in vitro fertilization operation, ready for in vitro culture of embryos. The involved fertilization culture fluid and semen preparation culture fluid contain ingredients such as sodium pyruvate and heparin. The method of the invention can significantly improve the efficiency of bovine in vitro fertilization.

The invention discloses a research method of kidney-tonifying pharmacological action of wood frog oil freeze-dried powder. The research method comprises the following steps: researching the influenceof the wood frog oil freeze-dried powder on the carbon particle clearance capacity of a mouse reticuloendothelial system, researching the influence of the wood frog oil freeze-dried powder on the weight indexes of the reproductive and adnexed organs including seminal vesicle, testis, prepuce gland, immune organ thymus and spleen of a young male mouse, researching the influence of the wood frog oilfreeze-dried powder on the endocrine function of a rat with kidney-yang deficiency, researching the influence of the wood frog oil freeze-dried powder on the weight indexes of the seminal vesicle andprepuce gland of a castrated mouse, researching the influence of the wood frog oil freeze-dried powder on the sperm count and the percentage of live sperms, and making overall evaluation according tothe research. The research method can be used for researching the wood frog oil freeze-dried powder from multiple aspects of kidney function influence to form a systematic evaluation method, and hasgreat significance for the development of a wood frog oil freeze-drying process and the popularization of freeze-dried products.

The invention discloses a traditional Chinese medicinal preparation for treating male asthenospermia and oligospermia and a preparation method. The traditional Chinese medicinal preparation is prepared from radix rehmanniae preparata, rhizoma dioscoreae, pulp of dogwood fruits, radix morindae officinalis, Chinese wolfberry fruits, herba epimedii, cortex moutan, herba hedyotis, rhizoma paridis, radix codonopsis, radix scutellariae, radix salviae miltiorrhizae, ants, corn cervi pantotrichum and semen allii tuberose. The preparation method comprises the following steps: preparing for the medicinal materials in the prescription of the preparation, carrying out screening, carrying out high-temperature distinction, carrying out drying, carrying out grinding to obtain powder, carrying out filtering, taking the powder, carrying out uniform mixing, and treating the mixture by adopting water to obtain pills. The traditional Chinese medicinal preparation can obviously change the symptom of kidney-yang deficiency, increase the sperm count, improve the sperm motility, improve the sperm quality, and improve the pregnancy rate.

The invention provides an optimized sperm counting method for an egg zona pellucida binding test. The method is easy and convenient to operate, used reagents are easy to obtain, the test cost can be remarkably reduced, and the technical problem that clinical popularization is limited due to the fact that operation is complex and the requirement for detection personnel is too high is solved.

The invention discloses a capsule for treating flaccidity and relates to the technical field of drug formulation. The Xianling capsule for treating flaccidity comprises the following raw materials (byweight): 188.1 parts of herba epimedii, 31.35 parts of pilose antler, 30.16 part of monkshood, 62.7 parts of cistanche, 94.05 part of semen cuscutae, 75.24 parts of Chinese chive seed, 62.7 parts ofRadix Rehmanniae Praeparata, 62.7 parts of Chinese yam, 62.7 parts of cornus officinalis, 94.05 parts of medlar, 62.7 parts of American ginseng, 62.7 parts of radix bupleuri, 37.62 parts of safflowerand 18.81 parts of honey-fried licorice root. The capsule has the effects of warming kidney, assisting yang, strengthening tendons and the like. The capsule is used for symptoms such as impotence, orlifting but not firm, premature ejaculation, soreness and weakness or pain of waist and knees, dizziness and tinnitus, pale complexion, mental fatigue, fear of wind and cold, kidney diarrhea, cold semen, frequent urination at night, low sperm count, poor sperm motility, low sperm survival rate, weak sperm penetration and the like, and contains pure traditional Chinese medicinal components withoutany toxic or side effects.