17 results about "Sperm counts" patented technology

The invention relates to the technical field of biological medicines, in particular to an umbilical cord mesenchymal stem cell exosome and application of the umbilical cord mesenchymal stem cell exosome in preparation of a medicine for treating male infertility caused by hidden testis. The prepared high-concentration umbilical cord mesenchymal stem cell exosome suspension preparation is directly used for treating cultured spermatogonial cells and spermatoblasts in vitro, is injected into the testis of a mouse, and is used for improving the seminiferous tubule and spermatogenic microenvironment of testis tissue and promoting division and proliferation of the spermatogonial cells and the spermatoblasts. The method can effectively reverse oligospermia and azoospermia caused by the hidden testis, and compared with a model without intervention of treatment means, the method can effectively promote increase and even regeneration of sperms in the testis.

The invention discloses application of exosome-like nanoparticles derived from fructus lycii to preparation of a medicine for preventing or treating oligospermia and / or asthenospermia. Sterile, stable and high-purity fructus lycii exosome-like nanoparticles (LBELNs) are obtained by adopting a differential centrifugation-suction filtration-ultra-high-speed centrifugation method, the physiological efficacy of the LBELNs is researched, and it is found that the LBELNs can significantly improve the oligoasthenospermia disease activity index of male mice induced by cyclophosphamide (CTX); the sperm quantity is obviously increased, the sperm malformation is improved, the sperm motility is improved, and the sex hormone level is recovered. The LBELNs can effectively relieve spermatogenic dysfunction of testicular tissues, the sperm differentiation and formation process is obviously improved, the total number of sperms is increased, and the treatment effect is more obvious along with increase of the dosage of the LBELNs. The invention has potential in preparation of drugs for preventing or treating oligoasthenospermia, and provides a new strategy and treatment method for treating oligoasthenospermia.

The invention discloses a traditional Chinese medicine composition for treating insufficiency of kidney yang and a preparation method thereof, and relates to the technical field of traditional Chinese medicines. The traditional Chinese medicine composition is prepared from the following components in parts by weight: 20 to 35 parts of rhizoma polygonati extract, 20 to 35 parts of semen astragali complanati, 20 to 35 parts of herba epimedii, 10 to 15 parts of rhizoma drynariae, 10 to 15 parts of fructus rubi, 10 to 15 parts of herba verbenae, 5 to 15 parts of rhizoma polygonati, 5 to 15 parts of cortex eucommiae and 5 to 15 parts of fructus psoraleae. Through scientific compatibility and an optimized preparation process, the curative effect of the traditional Chinese medicine composition in the aspect of treating kidney-yang deficiency is remarkably improved. Experimental results show that the traditional Chinese medicine composition can obviously improve the kidney-yang deficiency symptom, increase the sperm quantity and activity, reduce the sperm malformation rate and improve the sexual organ weight and the female mouse pregnancy rate. In addition, the traditional Chinese medicine composition can be used for preparing traditional Chinese medicine preparations of various dosage forms, and has wide application prospects.

The present application provides use of compounds represented by formula (I) and formula (II) in the preparation of drugs for improving male reproductive ability. In the present application, different oligoasthenospermia models are constructed, and the improvement effects of the compounds represented by formula (I) and formula (II) on male reproductive ability impairment are verified by means of sperm count analysis, sperm motility analysis, mRNA expression level analysis of inflammatory and antioxidant genes, testicular histopathological damage analysis, etc. The experimental results show that the compounds represented by formula (I) and formula (II) increase the sperm count in mice with oligoasthenospermia, improve the sperm motility of the mice with oligoasthenospermia, ameliorate testicular histopathological damage in the mice, suppress the expression of inflammatory factors, and increase the expression of antioxidant genes, demonstrating great potential in the treatment of oligoasthenospermia.

The invention discloses application of tremella aurantialba polysaccharide in preparation of a medicine for treating male immunological dyszoospermia, and belongs to the field of biological medicine. The invention provides a new application of tremella aurantialba polysaccharide, and experiments prove that the tremella aurantialba polysaccharide can improve dyszoospermia induced by cyclophosphamide, significantly increase the number of sperms of mice, reduce the abnormal rate of sperms and promote secretion of testosterone. According to the invention, cyclophosphamide is adopted to construct a male mouse dyszoospermia model, tremella aurantialba polysaccharides extracted in different manners are subjected to intragastric administration, the action mechanism of NAPs for improving mouse dyszoospermia is researched, the mechanism of NAPs for treating spermatogenesis is disclosed, and a basis is provided for clinical application.

The invention discloses a traditional Chinese medicine composition for treating infertility, which is prepared from the following raw materials: radix rehmanniae recen, radix paeoniae alba, turtle shell, cortex moutan, peach kernel, ginseng, radix glehniae, medlar, oyster, rhizoma cyperi, radix curcumae, astragalus membranaceus, angelica sinensis, rhizoma anemarrhenae, golden cypress, lotus seed, rhizoma atractylodis macrocephalae, pericarpium citri reticulatae, chicken's gizzard-membrane, radix bupleuri, tetrapanax papyriferus, donkey-hide gelatin, eucommia ulmoides and deer glue. Spleen tonifying, blood nourishing, phlegm reducing and dampness clearing; the effects of soothing liver-qi stagnation, promoting qi and activating blood are achieved; qi is benefited, blood is nourished, and Zhongzhou is promoted to regenerate Qi and blood; promoting blood circulation to remove blood stasis and dredging Chong and Ren qi; the traditional Chinese medicine composition has the effects of clearing heat, promoting diuresis, eliminating dampness and reducing phlegm, can achieve sufficient qi and blood of liver and kidney and smooth Chong and Ren channels, has a good treatment effect on male and female infertility caused by various reasons, and is particularly suitable for female infertility caused by kidney deficiency, weakness, fallopian tube blockage, cold uterus, kidney deficiency type ovulation disorder, obesity, stasis, weakness, blood deficiency and the like; and the traditional Chinese medicine composition is also suitable for male infertility caused by cold sperm, rare sperm, oligospermia and necrospermia due to kidney deficiency.

The application provides a hidden sperm screening system and method based on magnetic bead capture and fluorescent identification, and is applied to the technical field of magnetic bead fluorescence analysis. A sperm sample is incubated with high-magnetic-saturation first immunomagnetic beads and low-magnetic-saturation second immunomagnetic beads in an incubation module, and the two kinds of magnetic beads are coupled with anti-sperm antibodies and anti-non-sperm cell antibodies respectively. A magnetic field generating module applies an alternating magnetic field to a separation area and synchronously injects sheath liquid, and uses the difference in magnetic response characteristics to retain the complex combined with sperm and to flush away the complex combined with non-sperm cells. A dye introduction module introduces membrane impermeable and permeable dyes under the constraint of a magnetic field, and obtains fluorescent characteristics of membrane-intact and membrane-damaged sperm which can be distinguished. An imaging module performs multi-channel fluorescence imaging, an image analysis module extracts positive events conforming to morphology and fluorescent logic, and finally outputs sperm count parameters and membrane integrity classification parameters synchronously.

The utility model provides a sperm counting plate with double sperm counting chambers, which belongs to the technical field of sperm counting plates and comprises a bottom plate, the top of the bottom plate is provided with a first counting chamber and a second counting chamber, two sperm samples can be simultaneously accommodated for detection operation, and the problem that a traditional counting plate cannot simultaneously process a plurality of sperm samples is solved. Bearing assemblies are arranged at the bottoms of the first counting chamber and the second counting chamber, each bearing assembly comprises a counting bottom plate, the bottoms of the first counting chamber and the second counting chamber are detachably connected with the counting bottom plates, and objective tables for bearing sperm samples are arranged at the tops of the counting bottom plates; after detection is completed, the counting bottom plate can be detached and cleaned, meanwhile, the other counting chamber can continue to work, the detection process does not need to be interrupted, protection assemblies are arranged at the tops of the first counting chamber and the second counting chamber, each protection assembly comprises a cover plate, and the cover plates are detachably installed at the tops of the first counting chamber and the second counting chamber.

The invention discloses a method for constructing an azoospermia mouse model by knocking out a germ cell Rbbp6 gene and application of the azoospermia mouse model, the construction method is to knock out the Rbbp6 gene on a mouse germ cell genome, and the knockout is carried out by using a Cre-LoxP system. The azoospermia mouse model constructed by the invention can be used for research on non-obstructive azoospermia and corresponding drug research and development. The model mouse shows that the testis weight and volume are reduced, spermatogenic epithelium atrophy, a large number of germ cells are deleted, and the number of sperms is reduced.

A medical magnifying lens comprises a focusing module, a sperm counting chamber assembly, an optical lens, a light source circuit board and a main control circuit board which are arranged in an upper shell and a lower shell, a lifting shaft in the focusing module ascends and descends through transmission between a focusing gear and a first transmission gear, then the sperm counting chamber assembly is driven to ascend and descend, the focusing action is achieved, imaging is clear, and the imaging precision is high. The visual field range is adjustable, the visual field content can be observed through a mobile phone camera, the focusing assembly is additionally provided with a second transmission gear, the focusing assembly can be fully and flexibly matched with the space of the lower shell, the structure is compact, and the focusing stability is improved by limiting a first transmission gear and a lifting shaft; the magnifying lens is additionally provided with the liquefaction assembly, so that sample liquefaction, sample slide glass manufacturing and visual field observation can be realized, and the magnifying lens is complete in function, small in overall design, simple and easy to operate, easy to learn, high in functional stability and convenient to carry outside.

The application relates to the field of animal breeding technology, in particular to a method and system for detecting total live sperm count of a cow and application. The method comprises the following steps: based on the genome version ARS-UCD1.2, the methylation level of a region such as chr17:62205563-63205744 of a to-be-detected cow is detected; the detection result is compared with a reference standard, and the total live sperm count of the to-be-detected cow is obtained; the reference standard is obtained based on GWAS correlation data of the total live sperm count and the methylation level of a reference cow group in the region of chr17:62205563-63205744. A region significantly related to the total live sperm count of the cow is obtained through research, early identification of the total live sperm count of the cow can be realized by detecting the methylation level of the region, and this has important significance in the field of animal phenotype identification.

The invention discloses a method for constructing an azoospermia mouse model by knocking out a germ cell EIF5A gene and application of the azoospermia mouse model, the construction method is to knock out the EIF5A gene on a mouse germ cell genome, and the knockout is carried out by using a Cre-LoxP system. The azoospermia mouse model constructed by the invention can be used for research on non-obstructive azoospermia and corresponding drug research and development. The model mouse shows that the testis weight and volume are reduced, the spermatogenic epithelium is atrophic, spermatogonium cells cannot complete meiosis completely, germ cells are massively deleted, and the sperm quantity is reduced.

The invention discloses a method for constructing an azoospermia mouse model by knocking out a germ cell Sno1 gene and application of the azoospermia mouse model, the construction method is to knock out the Sno1 gene on a mouse germ cell genome, and the knockout is carried out by using a Cre-LoxP system. The azoospermia mouse model constructed by the invention can be used for research on non-obstructive azoospermia and corresponding drug research and development. The model mouse shows that the testis weight and volume are reduced, spermatogenic epithelium atrophy occurs, spermatogonium cells do not enter meiosis, germ cells are massively deleted, and the number of sperms is reduced.

The invention provides a cover plate type sperm counting device and method, and belongs to the technical field of sperm counting.The cover plate type sperm counting device comprises a sperm counting plate, a detection through groove is formed in the bottom of the sperm counting plate, a sample containing box is detachably connected to the bottom of the detection through groove through a connecting assembly, and a sample containing cavity is formed in the top of the sample containing box; a horizontal adjusting assembly is arranged at the bottom of the sample containing cavity and comprises an adjusting plate and four sets of electric telescopic rods used for adjusting the adjusting plate, the adjusting plate is arranged in the sample containing cavity, the top ends of the four sets of electric telescopic rods are connected with the four corners of the adjusting plate respectively, a glass slide and a cover glass are arranged at the top of the adjusting plate, and a gyroscope is arranged at the bottom of the adjusting plate. A cover plate assembly is arranged at the top of the sperm counting plate, and the bottom of the cover plate assembly is detachably connected with the cover glass; through the arrangement of the horizontal adjusting assembly, the horizontal state of the adjusting plate is monitored in real time through a gyroscope, and then four sets of electric telescopic rods are driven to perform telescopic action, so that the adjustment of the horizontal state of the adjusting plate is realized.

The invention provides application of a compound shown in a formula (I) in preparation of a medicine for improving male reproductive capacity. According to the application, a nano micro-plastic and plasticizer induced Kunming mouse environment pollution type oligospermia and asthenospermia model, a streptozotocin induced Kunming mouse diabetes type oligospermia and asthenospermia model and a sodium valproate induced Kunming mouse anti-epileptic drug type oligospermia and asthenospermia model are respectively constructed; the improvement effect of the compound shown in the formula (I) on male reproductive capacity damage is verified through sperm counting analysis, sperm motility analysis, inflammation and antioxidant gene mRNA expression level analysis, testis tissue pathological damage analysis and the like, and experimental results show that the compound shown in the formula (I) increases the sperm number of mice suffering from oligospermia and asthenospermia, and has a good application prospect. According to the application, the sperm motility of mice with oligospermia and asthenozoospermia is improved, the tissue pathological damage of the testis is improved, the expression of inflammatory factors is inhibited, the expression of antioxidant genes is increased, and the application has great potential in the aspect of treating oligospermia and asthenozoospermia.

The present disclosure describes methods of reducing the likelihood of conception following intercourse between a male subject and a female subject, methods of producing reversible infertility in a male subject, methods of reducing sperm count in a male subject, and methods of selectively antagonizing RAR alpha over RAR beta and RAR gamma in a subject.

The invention discloses application of polydatin in preparation of a medicine for improving and repairing cryptorchis spermatogenic function damage, and belongs to the technical field of biological medicine. The medicine takes polydatin as a main active ingredient. Experiments prove that the polydatin can effectively improve the spermatogenic function, promote the reproductive repair of a mouse with cryptorchis and improve the hormone release level of serum testosterone and gonadotropin, the hormone is beneficial to spermatogenesis, the repair effect has a dose effect, and the repair effect is optimal when the polydatin concentration is 50-100mg / kg. In addition, metabolome in the testis of the mouse can be adjusted, oxidative stress and inflammatory response are relieved, and therefore sperm counting and epididymis weight are affected and improved. According to the scheme, an experimental basis is provided for application of polydatin in the field of reproductive health, and a new candidate medicine is also provided for treatment of male infertility such as hidden testis.