Reagent for detecting sperm membrane surface antigen by immuno-magnetic sand method, its preparation and detection method

A technology for detecting reagents and immunobeads, which is applied in the field of sperm membrane surface antibody immunobead method detection reagents and its preparation and detection. It can solve the problems of different sizes of immune beads, difficult to obtain, and small males, and overcome the inability to directly detect , The method is simple and easy to implement, and the effect is easy to interpret
CN1755367AInactive Publication Date: 2006-04-05深圳华康生物医学工程有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
深圳华康生物医学工程有限公司
Publication Date
2006-04-05
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a semen film surface antibody immunity method testing agent and preparing and testing method. The semen film surface antibody immunity method testing agent comprises: 1.8um-2.2um immunity bead which can possesses human antibody IgG or IgA or IgM micromole rubber latex particle, the washing liquid which contents 0.1%-0.5% bonnie serum albumin and the scale dissolver which contents 4%-6% bonnie serum albumin. The agent can also comprise semen extracting liquid with high activity. The invention provides the method for preparing the semen film surface antibody immunity method testing agent.
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Description

【Technical field】

[0001] The invention relates to a reagent for in vitro diagnosis and its preparation and detection method, in particular to a sperm membrane surface antibody immune bead method detection reagent and its preparation and detection method. 【Background technique】

[0002] Immune factors are one of the causes of male infertility, so its clinical detection is a very important topic. In 2000, the World Health Organization (WHO) stipulated that mixed agglutination reaction (MAR) and immunobead test (IBT) were standard tests for diagnosing immune infertility.

[0003] At present, due to the difficulty in obtaining the detection reagents of the immunobead method, enzyme-linked immunoassay (ELISA) is mainly used in China to detect anti-sperm antibodies in serum or seminal plasma. This test is of little significance to men, and the false positive rate is high. . In the actual operation of the immune bead test, there are also differences in the size of the immune bead...

Claims

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