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Proteins relating to grain shape and leaf shape of rice, coding genes and uses thereof

a technology of rice and grain shape, applied in the field of biotechnology, can solve the problems of conflicting global rice supply and demand, low rice output under large-scale production, etc., and achieve the effects of increasing grain length, increasing grain weight, and increasing grain weigh

Active Publication Date: 2012-09-20
INST OF SUBTROPICAL AGRI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0017]The plant expression vector to be used may be processed, for example, by introducing gene that is expressed in the plant to produce a color-changeable enzyme or a luminous compound (GUS gene, GFP gene, luciferase gene etc.), an antibiotic marker with resistance (gentamicin marker, kanamycin marker etc.) or a marker gene for an anti-chemical reagent (e.g., anti-herbicide gene) and the like, for convenient identification and screening of a transgenic plant cell or plant.

Problems solved by technology

With the increasing growth of global population (the population growth rate of rice-consuming countries is faster than the average growth rate of the world population) and rapid development of industrialization and urbanization as well as damages caused by natural disasters and the like, there is an decreasing trend in rice paddies, causing a pressing conflict between the global rice supply and demand.
However, the rice output under large-scale production is generally very low.

Method used

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  • Proteins relating to grain shape and leaf shape of rice, coding genes and uses thereof
  • Proteins relating to grain shape and leaf shape of rice, coding genes and uses thereof
  • Proteins relating to grain shape and leaf shape of rice, coding genes and uses thereof

Examples

Experimental program
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Effect test

example 1

Breeding of a Transgenic Rice Having Long, Large and Plural Grains and Rolled Leaf

I. Construction of Recombinant Expression Vector

1. Cloning of OsXCL Gene

[0044]A pair of primers was artificially synthesized whose 5′ terminals were added with NcoI and BamHI enzymatic cleavage sites respectively. The upstream primer and downstream primer are as follows:

F: 5- GAATCCAATCCACTCCACTCCACC-3 (30);R: 5- CTAATAGGCGGTGTGGTGTTGCG-3 (29).

[0045]RNA, extracted from rice Pei'ai 64S (Chinese National Center for Rice Hybridization and Breeding, Changsha, China), was reversely transcribed to give the first chain of cDNA.

[0046]A PCR was performed to amplify cDNA as set forth by SEQ ID NO: 1 with the above upstream primer and downstream primer as primers and cDNA of rice Pei'ai 64S as a template. The PCR program started with a pre-denaturation at 94° C. for 5 min under a hot lid of 105° C., followed by 32 cycles of denaturation at 94° C. for 30s, annealing at 56° C. for 30s, extension at 72° C. for 1 min...

example 2

Breeding of Transgenic Arabidopsis thaliana

[0072]A recombinant expression vector OsXCL-163-1300 was obtained according to the method provided in Example 1, and then integrated into the genome of Arabidopsis thaliana (Col-0, commercially available from Arabidopsis Biological Resource Center, ABRC) through floral-dip method mediated by Agrobacterium; screening was performed on a MS medium containing hygromycin (50 mg / l), seeds from T2 generation were continuously screened on the MS containing hygromycin, and plant lines with the segregation ratio being 3:1 were chosen for continuous screening in T3 generation; if 100% of the seeds from T3 generation may grow on a MS containing hygromycin, this plant line was deemed as homozygous line, and a transgenic Arabidopsis thaliana overexpressing OsXCL gene was obtained.

[0073]Meanwhile, Arabidopsis thaliana transformed with expression vector 163-1300 free of OsXCL gene was used as an empty vector control.

[0074]The T3 generation of transgenic A...

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Abstract

The present invention provides a protein OsXCL relating to grain shape and leaf shape of rice, its derived proteins and their coding genes. Transgenic rices over-expressing OsXCL gene present phenotypes as increase of grain length, grain weight and number of grains per panicle, and leaf rolling and so on. The present invention also provides the method for obtaining transgenic plants by transforming the coding genes of OsXCL or its derived proteins into objective plants.

Description

FIELD OF THE INVENTION[0001]The present invention relates to the field of biotechnology, particularly, to grain length, grain weight, grain number per panicle and rolled leaf-related protein in rice, encoding gene thereof and use of the same.DESCRIPTION OF BACKGROUND[0002]Rice is one of the main grain crops on which the life of human being depends, provides staple food for nearly one half of the world population. In China, rice stands first among all grain crops in production and accounts for 60% of grain ration consumption of Chinese residents; nearly half of the farmers are engaged in rice production. Accordingly, rice plays a leading role in Chinese food crops. With the increasing growth of global population (the population growth rate of rice-consuming countries is faster than the average growth rate of the world population) and rapid development of industrialization and urbanization as well as damages caused by natural disasters and the like, there is an decreasing trend in ric...

Claims

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Application Information

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IPC IPC(8): C07K14/415A01H1/00C12N15/70C12N15/29C12N1/21
CPCC12N15/8261C07K14/415Y02A40/146
Inventor XIA, XINJIE
Owner INST OF SUBTROPICAL AGRI CHINESE ACAD OF SCI
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